| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,805,733 | 0 | T | G | 53.3% | 6.7 / 13.0 | 15 | intergenic (+153/‑596) | dsbG/CJ019_04513 | Thiol:disulfide interchange protein DsbG/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (0/8); total (2/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATT‑CGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTGG > KT2440/4805603‑4805857 | gacccccGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGc < 7:139053/130‑1 (MQ=255) gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTAGGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTa < 4:84144/135‑1 (MQ=255) ccGCGACGCCGGCGACGGGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGGGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAg < 6:201751/135‑1 (MQ=39) accGGGGCGCGCGCTAAGGGCGGGGTTTTGCGCCAGGCCCGTGAGCGCACGGCCTGCGCGGGCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgc < 8:102962/133‑1 (MQ=21) ggggCGCGCGCTACCGGCGGGGTGTGGCCCCAGCCCTGTTATCGAAAGGCTTGTGCTGGCGCCCTTATTTGCGGGTTTACCCTCGAAATGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgc < 4:202065/135‑1 (MQ=9) cgcgCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCAc > 1:215342/1‑135 (MQ=255) cTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTa < 2:215342/135‑1 (MQ=255) tacgggCGGGGTTTGGCGCCCGGCCCGTTAGCCCACGCCCTGCGCGGGCGGCGGTAGGCGGGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTAt < 2:35364/131‑1 (MQ=17) aaagggCGGGGTTTTGCGCCAGGCCGGGGAGCGCACGGCCGGCGGGGGCGGCCGTTGGAGGGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTAt < 8:166759/131‑1 (MQ=18) gcggggTTTTGCGCCTGGCCGGTGATCCCACGCCCGGCGCGGGCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATT‑cgcg < 7:167542/132‑1 (MQ=17) cggCCCGTGAGCGCACGGCCTGCGCGGGCGGCCGTAGGAGGGGGTTAACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATT‑CGCGGGTAAACCCGCTCCt < 2:279219/134‑1 (MQ=17) gattggCCGGGGCGGGCGGCCTTTGGTGGGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATT‑CGCGGGTAAACCCGCTCCTACGCTGATCgg < 4:117148/130‑1 (MQ=17) aaGGCCTGCGCGTTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATT‑CGCGGGTAAACCCGCTCCTACGCTGATCggggg < 7:61993/135‑1 (MQ=255) ggCCTGCGCGGTCGGCCGTTGGAGCGGGTTTTACCGCGAAAGGGCCCGGCCAGCGGGGCAAATTACACCCGCCCGTGACCGGTTGCGCCGCCCCAGTATTACACGGGTAAACCC‑CGCCCAAGCGGATCggggggg > 8:319063/1‑133 (MQ=255) gCCGGCGCGGGCGGGGGTCGGCGGGGGTTAACCCGCGAAAGGGCCGGCCCTGCGGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATT‑CGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTgg < 3:261222/135‑1 (MQ=14) | GAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATT‑CGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTGG > KT2440/4805603‑4805857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |