| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,262,767 | 0 | A | C | 58.3% | ‑5.9 / 12.4 | 24 | intergenic (+9/+27) | uacT_2/radA | Uric acid transporter UacT/DNA repair protein RadA |
| Reads supporting (aligned to +/- strand): ref base A (6/4); new base C (11/3); total (17/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCGTGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAG‑CC‑GGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGAT > KT2440/5262634‑5262892 | tGCGTGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAg < 2:206856/135‑1 (MQ=255) gATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCTCTGACGGGTTGCCggcg > 1:207843/1‑131 (MQ=255) gATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGgag > 7:20103/1‑133 (MQ=255) ctactTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGAGCGAGCTTgct > 6:151727/1‑135 (MQ=255) actTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGGGCGCGCGTggtcg > 1:206403/1‑135 (MQ=255) ttCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGGGCGCGCATtctcgca > 2:263180/1‑134 (MQ=255) cAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACTGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGGGCGAGCGggctggctgc > 7:289992/1‑133 (MQ=255) aTGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGCGCGAGCTTGCTCGCTCCAGCAc > 7:8013/1‑135 (MQ=255) aaGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCCAGCAAGGTAGCCCCAGCACGCTAt > 6:175888/1‑135 (MQ=255) aGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGGTCGCTCCTGCACGcga > 1:217839/1‑131 (MQ=255) aGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGGACGCGAtt > 1:212912/1‑135 (MQ=255) cgTGCCGAGGGGGGAAGTGCACGACCCCGGAAATGAACCGGCCTGGCGCCGGGCGGTGGCCGACCACTTAGGGTTTGCCGGAGCGAGCTGGCTCGCTCCGGCACGCTATTCGAACAATGCATCCAGCGCCTGTTc < 4:100890/134‑1 (MQ=255) cGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTTCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAG‑cc > 6:286985/1‑135 (MQ=255) aCTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGGCGGCGCGCGCGTGGTCGCGCCGGGCCGCGATTGGGACAAAGCATGCCGCGCCGGGTCCAC‑CC‑CGGGGACgg > 5:275600/1‑134 (MQ=255) gAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGAGCGAGCTTGGTGGCGGCGGGCCGCTATTCGAACAATGCATCCCGCCCCAGGTACAG‑CC‑GGGGTACGGCAATCa > 2:122438/1‑133 (MQ=255) gAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGAGCCCGCTTTGTAGCGGCAGCACCCGTTTAGAACAAATCATCCAGCCCCAGTTACAGCCC‑GGGTAA‑GGCCATCa > 1:270409/1‑133 (MQ=255) gCACGGCAATGAAACGGCCCGCCGCACGCCCGTTGCCGAGCACTGACGGGTGGCAGGCGCGAGCTTGCGCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAG‑CC‑GGGTAACGGCAATCACCTg < 8:20103/135‑1 (MQ=255) cGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGCGCGCGCTTGCTAGCTCCTGCACGCGATTGGAACAATGCATACAGCGCCTGTTGCAG‑CC‑GGGGTACGTCAATCACCTGcca > 8:284282/1‑133 (MQ=255) aaCGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCCGGGGCGGGCGTGGGGGGGCCTGGCCCCGATTGGAACAATGCATCCCCCCCCGGGTGCCG‑CC‑GGGGTACGGGCATCACCCGCCAccccccccg > 6:331469/1‑130 (MQ=255) gCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAG‑CC‑GGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCa < 3:143470/135‑1 (MQ=255) ccGAGCACTGACGGGTTGCCGGGGCGAGCGTGCGGGCTGCGGGCCGCGATTAGAACAATTGATCCCCCGCCTGGTGCAG‑CCGGGGTAAC‑GCCATGACCCGCCACCCCGCCCGCGCGTCGTTGGGCGCCTTGgccc > 4:232356/1‑134 (MQ=255) cgcgCACTGAGGGGTGGCCGCCGCGCGCTGGCGCGCGCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAG‑CC‑GGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCtt < 2:217839/132‑1 (MQ=255) cccccACTGAGGGGTTGCAGCAGCGCCCTGGCGCGCCCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAG‑CC‑GGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCtt < 2:212912/131‑1 (MQ=255) gggtgtGCCGGAGCGACCTGGCCCGCGCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAG‑CC‑GGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGAt < 1:263180/129‑1 (MQ=255) | TGCGTGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCAGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAG‑CC‑GGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGAT > KT2440/5262634‑5262892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |