Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 1,807,474 G→A G258D (GGC→GAC)  yeiG → S‑formylglutathione hydrolase YeiG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,807,4740GA96.4% 183.0 / ‑1.3 55G258D (GGC→GAC) yeiGS‑formylglutathione hydrolase YeiG
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (27/26);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

AGGCCAATGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCACAGC  >  KT2440/1807343‑1807599
                                                                                                                                   |                                                                                                                             
aGGCCAATGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTa                                                                                                                            >  6:27461/1‑135 (MQ=255)
  gCCAATGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATg                                                                                                                          <  3:172562/135‑1 (MQ=255)
     aaTGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGAcc                                                                                                                       >  5:37597/1‑135 (MQ=255)
     aaTGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGAcc                                                                                                                       <  3:114403/135‑1 (MQ=255)
       tGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGAcc                                                                                                                       >  4:43813/1‑133 (MQ=255)
          ccgccgTTGCTGGTGGATCCGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGc                                                                                                                  <  1:333902/135‑1 (MQ=255)
          ccgccgTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATg                                                                                                                          >  7:139481/1‑127 (MQ=255)
          ccgccgTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATg                                                                                                                          <  8:139481/127‑1 (MQ=255)
          ccgccgTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAg                                                                                                                   >  3:191617/1‑134 (MQ=255)
               gTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGCCCATAGctacta                                                                                                             >  6:171064/1‑135 (MQ=255)
               gTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGctacta                                                                                                             >  6:82554/1‑135 (MQ=255)
               gTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGctacta                                                                                                             >  6:100633/1‑135 (MQ=255)
                 tGCTGGGGGATCAGGGCGAGCGTGACGACTTCCTGGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGACAGACTATGACCATAGCTactact                                                                                                           <  7:334849/135‑1 (MQ=255)
                    tggtggATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCa                                                                                                        >  8:230458/1‑135 (MQ=255)
                     ggtggATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCAt                                                                                                       <  5:100633/135‑1 (MQ=255)
                          aTCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCa                                                                                                  <  7:81306/135‑1 (MQ=255)
                           tCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAg                                                                                                 <  6:262694/135‑1 (MQ=255)
                                gcgcCCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCGTCa                                                                                            >  6:49057/1‑135 (MQ=255)
                                 cGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGAc                                                                                                                        <  2:127347/106‑1 (MQ=255)
                                 cGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGAc                                                                                                                        >  1:127347/1‑106 (MQ=255)
                                   aCCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCg                                                                                         <  4:191617/135‑1 (MQ=255)
                                    ccGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCga                                                                                        >  3:232325/1‑135 (MQ=255)
                                     cGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCgag                                                                                       <  1:286076/135‑1 (MQ=255)
                                      gTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCga                                                                                        >  7:96214/1‑133 (MQ=255)
                                      gTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCga                                                                                        >  1:287583/1‑133 (MQ=255)
                                        gacgacTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCgaggag                                                                                    <  6:240325/135‑1 (MQ=255)
                                             cTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCt                                                                               <  8:173142/135‑1 (MQ=255)
                                                 cTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTgcg                                                                            >  6:281320/1‑134 (MQ=255)
                                                   cGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCca                                                                         >  7:32752/1‑135 (MQ=255)
                                                        aGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATg                                                                    <  4:11707/135‑1 (MQ=255)
                                                        aGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATg                                                                    <  2:212697/135‑1 (MQ=255)
                                                                   ccTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGATCACCTGCGCCATCATGCGGTGGCACTg                                                         <  7:209368/135‑1 (MQ=255)
                                                                   ccTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTg                                                         <  3:92190/135‑1 (MQ=255)
                                                                          cgcTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCtgg                                                  >  2:302987/1‑135 (MQ=255)
                                                                          cgcTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGACggg                                                  >  7:156956/1‑135 (MQ=255)
                                                                             tGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGttt                                               <  5:281320/135‑1 (MQ=255)
                                                                               gAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCca                                                                         <  6:153937/107‑1 (MQ=255)
                                                                               gAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCca                                                                         >  5:153937/1‑107 (MQ=255)
                                                                                accAGGCGGCACGCAAATGTGGCCAGGCAATGACCCTGCGTCTGCAGCCAGACTCTGCCCATAGCTACTACTTCCTTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGGTTa                                              <  6:217530/131‑1 (MQ=255)
                                                                                aaCAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAAc                                            <  8:96214/135‑1 (MQ=255)
                                                                                 aCAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACg                                           >  2:195294/1‑135 (MQ=255)
                                                                                             cAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGa                               >  6:231628/1‑135 (MQ=255)
                                                                                                        aTGCAATGACCCTGCGTATGCAGCCAGACTATGACCAt                                                                                                                     >  7:303246/1‑38 (MQ=255)
                                                                                                        aTGCAATGACCCTGCGTATGCAGCCAGACTATGACCAt                                                                                                                     <  8:303246/38‑1 (MQ=255)
                                                                                                        aTGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGcc                    >  5:40575/1‑135 (MQ=255)
                                                                                                        aTGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGcc                    >  3:106004/1‑135 (MQ=255)
                                                                                                             aTGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGa               >  6:322061/1‑135 (MQ=255)
                                                                                                              tGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCAGCATGCGGTGGCACGGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGaa              <  6:37597/135‑1 (MQ=255)
                                                                                                               gACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGccc                   <  7:246838/129‑1 (MQ=255)
                                                                                                               gACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGccc                   >  8:246838/1‑129 (MQ=255)
                                                                                                                      cGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGca      <  8:251865/135‑1 (MQ=255)
                                                                                                                       gTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGcac     >  8:305783/1‑135 (MQ=255)
                                                                                                                         aTGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGcaca    >  7:337720/1‑134 (MQ=255)
                                                                                                                           gCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCAcagc  <  4:232325/134‑1 (MQ=255)
                                                                                                                           gCAGCCAGACTATGACCATAGCTACTAATTCATTGCCAGGTTCATCGAGGAGCACCTGCGCCATCATGCGGGGGAACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCAcagc  <  5:27461/134‑1 (MQ=39)
                                                                                                                                   |                                                                                                                             
AGGCCAATGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCACAGC  >  KT2440/1807343‑1807599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑