Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	2,031,520	G→T	T339T (ACC→ACA)	wecB ←	UDP‑N‑acetylglucosamine 2‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,031,520	0	G	T	100.0%	130.2 / NA	37	T339T (ACC→ACA)	wecB	UDP‑N‑acetylglucosamine 2‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/12);  total (25/12)

GGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGC  >  KT2440/2031405‑2031643                                                                                                                    |                                                                                                                            ggTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACaa                                                                                                          >  7:222893/1‑135 (MQ=255) ggTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACaa                                                                                                          >  7:265637/1‑135 (MQ=255) ggTGGGGGGGGGGCCAGGTGCCCAGGGATTTGCGTATGCGCGCGCAGGCTTTGCCAGCGCCATAGGGGTTATGGGCGAAGGTCATTTCGCGAAACGCCGCCTCAGCGTTCCGCAATGTCCTCCACGCCCGCCCaa                                                                                                          >  8:94654/1‑135 (MQ=255)  tgggggggggggCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAAt                                                                                                         >  3:45259/3‑135 (MQ=255)                         gggCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGc                                                                                                                  <  7:157923/103‑1 (MQ=255)                         gggCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGc                                                                                                                  >  8:157923/1‑103 (MQ=255)                         gggCTTAGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATCCTACTTCGGTGCcaacca                                                                                  >  7:334810/1‑135 (MQ=255)                                         cgcgcAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTgc                                                                  >  8:7336/1‑135 (MQ=255)                                           cgcAGGCTTTGCCATCGCCATAGGGGTTATGGGCTAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTgccg                                                                <  8:265637/135‑1 (MQ=255)                                                       cATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTg                                                    >  2:278518/1‑135 (MQ=255)                                                        aTCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCACCGCCCGCACACTCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTgg                                                   <  8:302891/135‑1 (MQ=255)                                                        aTCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTgg                                                   <  4:45259/135‑1 (MQ=255)                                                          cGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGgc                                                 <  2:207358/135‑1 (MQ=255)                                                             cATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGc                                                                                        >  8:272129/1‑93 (MQ=255)                                                             cATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGc                                                                                        <  7:272129/93‑1 (MQ=255)                                                             cATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCTACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCt                                              >  7:320421/1‑135 (MQ=255)                                                             cATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCt                                              >  8:225120/1‑135 (MQ=255)                                                                          ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACt                                                                                                                        <  6:55183/48‑1 (MQ=255)                                                                          ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACt                                                                                                                        >  5:55183/1‑48 (MQ=255)                                                                          ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTc                                                      >  6:316813/1‑114 (MQ=255)                                                                          ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTc                                                      <  5:316813/114‑1 (MQ=255)                                                                          ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTcgcg                                 >  2:105447/1‑135 (MQ=255)                                                                          ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTcgcg                                 >  5:241549/1‑135 (MQ=255)                                                                          ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTcgc                                  >  5:280557/1‑134 (MQ=255)                                                                          ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTcgc                                  >  2:195782/1‑134 (MQ=255)                                                                          ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTcgc                                  >  3:112568/1‑134 (MQ=255)                                                                          ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTcgc                                  >  3:269105/1‑134 (MQ=255)                                                                          ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTcgc                                  >  8:229876/1‑134 (MQ=255)                                                                          ggCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTcgc                                  >  6:291021/1‑134 (MQ=255)                                                                                        cgcgATACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGAGTCGCGCATCACCAGCACCg                   >  4:328827/1‑135 (MQ=255)                                                                                            aTACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGttt               <  4:112568/135‑1 (MQ=255)                                                                                            aTACGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGttt               <  7:229876/135‑1 (MQ=255)                                                                                              aCGCCGCCTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGc             >  6:288639/1‑135 (MQ=255)                                                                                                 ccgccTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCAcc                    <  8:127032/125‑1 (MQ=255)                                                                                                 ccgccTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCAcc                    >  7:127032/1‑125 (MQ=255)                                                                                                 ccgccTCATCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCa          >  7:188672/1‑135 (MQ=255)                                                                                                         tCGGTCAGCAATGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGgc  <  1:195782/135‑1 (MQ=255)                                                                                                                    |                                                                                                                            GGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGC  >  KT2440/2031405‑2031643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑