Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	2,839,289	C→T	intergenic (‑117/‑88)	CJ019_02692 ← / → CJ019_02693	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,839,289	0	C	T	100.0%	153.4 / NA	42	intergenic (‑117/‑88)	CJ019_02692/CJ019_02693	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/17);  total (25/17)

GGCACTGCAATGACATGGGGCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCCGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTGAGGCAGCAGT  >  KT2440/2839157‑2839405                                                                                                                                     |                                                                                                                     ggCACTGCAATGACATGGGGCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGATAGTCTgt                                                                                                                    <  2:195158/135‑1 (MQ=255)        gaaTGACATGGGGCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGcg                                                                                                             >  3:219568/2‑134 (MQ=255)         aaTGACATGGGGCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCt                                                                                                             >  8:55977/1‑134 (MQ=255)          aTGACATGGGGCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTc                                                                                                           <  1:311819/135‑1 (MQ=255)          aTGACATGGGGCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTc                                                                                                           <  5:113371/135‑1 (MQ=255)                   ggCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATc                                                                                                  >  8:133608/1‑135 (MQ=255)                   ggCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATc                                                                                                  >  8:23680/1‑135 (MQ=255)                   ggCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATc                                                                                                  >  3:125665/1‑135 (MQ=255)                          ctcctTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGAc                                                                                           >  1:128194/1‑135 (MQ=255)                          ctcctTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGAc                                                                                           >  4:59624/1‑135 (MQ=255)                          ctcctTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTAGTTGAAAGTCTGTGTTGGCTTCAAGCTCCTCCGGGGAc                                                                                           >  7:247143/1‑135 (MQ=255)                              ttGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGa                                                                                       <  2:96886/135‑1 (MQ=255)                                 gTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAACGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGAt                                                                                      <  6:98234/133‑1 (MQ=255)                                 gTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGAt                                                                                      <  7:55977/133‑1 (MQ=255)                                       aTGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGAt                                                                               >  7:143329/1‑134 (MQ=255)                                          tCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGAt                                                                               >  7:51316/1‑131 (MQ=255)                                          tCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGAt                                                                               >  6:92714/1‑131 (MQ=255)                                               gCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCg                                                                      >  5:52141/1‑135 (MQ=255)                                               gCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCg                                                                      >  7:300602/1‑135 (MQ=255)                                               gCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATACAGATTc                                                                       >  8:326486/1‑134 (MQ=255)                                                  gTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTcc                                                                   <  3:202570/135‑1 (MQ=255)                                                      tgtgtgGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCCTCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCa                                                               >  4:138717/1‑135 (MQ=255)                                                      tgtgtgGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCa                                                               >  5:80249/1‑135 (MQ=255)                                                      tgtgtgGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCa                                                               >  6:53702/1‑135 (MQ=255)                                                         gtgGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAAc                                                             <  2:128194/134‑1 (MQ=255)                                                           ggCTGAGAATTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCTTCCTGCAACCCt                                                          >  8:330431/1‑135 (MQ=255)                                                             cTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATACAGATTCGTCCTGCAACCCTCg                                                        >  5:171000/1‑135 (MQ=255)                                                              tGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCACCCCTCgg                                                       >  3:21135/1‑135 (MQ=255)                                                                  aTTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCaa                                                   <  6:171000/135‑1 (MQ=255)                                                                   ttttttCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCa                                                    >  5:335881/1‑133 (MQ=255)                                                                   ttttttCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCa                                                    >  1:220362/1‑133 (MQ=255)                                                                                         cccAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATg                            <  3:59624/135‑1 (MQ=255)                                                                                            aGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCg                          >  1:205217/1‑134 (MQ=255)                                                                                               tataAAATAAAAATCTGACTTATCTCATTTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGAATCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAAc                      <  4:263510/135‑1 (MQ=255)                                                                                                    aatataaCTCTGACTTATATGTATTTGAATGTCTGTGTTGGAGTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTAGTCCTTCAACCCTCGGGCTATAGGGGCAAAGAGGTTTTCTATGCGAAACAtctt                 <  7:330431/135‑1 (MQ=255)                                                                                                    aataaaaATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACAtctt                 <  5:283092/135‑1 (MQ=255)                                                                                                       aaaaaTCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTtctc              >  2:77227/1‑135 (MQ=255)                                                                                                         aaaTCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTtctc              >  2:322333/1‑133 (MQ=255)                                                                                                         aaaTCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTg            <  4:125665/135‑1 (MQ=255)                                                                                                           aTCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTGAg          <  6:335881/135‑1 (MQ=255)                                                                                                                 cTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGC‑AAGAGGTTTTCTATGCGAAACATCTTCTCTGAGgca       <  8:16344/131‑1 (MQ=255)                                                                                                                     tctcTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTGAGGCAGCAGt  <  2:220362/133‑1 (MQ=255)                                                                                                                                     |                                                                                                                     GGCACTGCAATGACATGGGGCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCCGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTGAGGCAGCAGT  >  KT2440/2839157‑2839405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑