| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 3,061,793 | G→A | S592N (AGC→AAC) | qedA_2 → | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,061,793 | 0 | G | A | 100.0% | 57.5 / NA | 20 | S592N (AGC→AAC) | qedA_2 | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/5); total (15/5) | |||||||||||
TTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTT > KT2440/3061661‑3061915 | tttCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg < 4:28409/135‑1 (MQ=255)tttCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg < 6:127495/135‑1 (MQ=255) aGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCACGGCGGCATTCTCTGGACCGTCAAGGTGCCCAACCAGTTTCAGCAAACCGCAAACGTCAAGc > 8:218993/1‑134 (MQ=255) aGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACGTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGc > 8:209079/1‑134 (MQ=255) tcCGGCTGGGGCGGCGCGGTGCCGCGGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCACTTTCAGCAAACGGCAAACGTCAAGCCaaaa > 2:305628/1‑135 (MQ=255) ccGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAAc > 7:164046/1‑135 (MQ=255) gTGCCGCTGTGGGGCGGCGAAGTTGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCt < 2:255129/135‑1 (MQ=255) ggggCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCt < 8:25476/135‑1 (MQ=255) cgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCGGCCGGAGATCggg > 2:330464/1‑134 (MQ=255) cgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCGTGCAGCCGCAGATAggg > 2:168815/1‑134 (MQ=255) aGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCAGAACGCCTGCCTACTGCCGCGTGCTGCCGCGGATAGGTTGCAAGCGGCCCTACTAcc > 2:323323/1‑134 (MQ=255) aGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTAAAGCCATAACGCCTGCCTACAGCCGCTTGCTCCCGCAGATAGCTGTCAAGCGGCCCAACAAcc > 7:14783/1‑134 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTTCTGCCGCTGAACGGTGGCAAGCGGCCCTACGACCAAATGACg > 1:77622/1‑133 (MQ=255) ggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa > 7:310310/1‑63 (MQ=255) ggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa < 8:310310/63‑1 (MQ=255) ggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGCTCGGTGGCCAGCGGCCCGACTACCAAATGACGAGAGCCCCGc > 1:213428/1‑135 (MQ=255) gcATGCTCTGGACCTTCAAGTTCCCCAAGCAGTTGCAGCAAACGGCAAACGACAAGCCATAACTCCTGCCTACTCCCGCTTGCTGCCGCTGATAGGTGGCAAGCGGCCCTACTACCAAATCACGAGAGccaagct > 4:97119/1‑130 (MQ=255) cTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCGGCTCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCa > 5:102686/1‑134 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCACTTGCTGCCGCTGATCGGTGGc < 7:67765/82‑1 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCACTTGCTGCCGCTGATCGGTGGc > 8:67765/1‑82 (MQ=255) cccAGTTGCAGAAACCGCCAAACGGCAACCCAAAACGCCGCCCACCTCCCGCTTGCTGCCGCTGAGCGGTGGCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcgcg < 7:282112/133‑1 (MQ=255) agcaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTAACAAATGCCGAGAGCCCCGCTGCCACCGATGCATTCTCCCGGCACGCGGGGattctt > 3:43528/1‑135 (MQ=255) | TTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTT > KT2440/3061661‑3061915 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |