Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	3,440,343	A→C	S18R (AGC→CGC)	CJ019_03266 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,440,343	0	A	C	90.9%	23.8 / ‑6.2	22	S18R (AGC→CGC)	CJ019_03266	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base C (16/4);  total (18/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGT  >  KT2440/3440210‑3440459                                                                                                                                      |                                                                                                                     cGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCACGGCCCTCTACACGCGCGGGGGCGCGGTGGTGCAGTGCGCGCTTGGGCCACCCGCCGGGGCGCGGTGGCGCTGCTGCTGGCCGCCa                                                                                                                      <  4:318550/134‑1 (MQ=255)                taaaTGGTGGTAGCAACTGGCTGGAGGAGCGCTAGGCCCTCTACCCGCGCGCGCGCGCGGTGCTGCAGTGAGCGCTTGGGCCCCGCGCGTGGTCGCCGGGGGGCTGGTGCTGGCCGCCCGGGCTGGCGGCGcc                                                                                                        <  8:51512/131‑1 (MQ=255)                         gtAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGCGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCCGGCCGCGCCCGGgccgccgc                                                                                             >  5:202704/1‑133 (MQ=255)                            gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTgggg                                                                                          >  4:252353/1‑134 (MQ=255)                            gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCAGGCGGCGCCCCGGCCGCCggggg                                                                                          >  3:227274/1‑134 (MQ=255)                                        aggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCCCGGGCGCCCCCCGCGCGCCCCGGGGGTGGCAGGCCac                                                                              >  1:107882/1‑134 (MQ=255)                                          gaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGcccccg                                                                            >  5:58941/1‑131 (MQ=255)                                                aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCAGCGGGGGCGGCGCCCCGGCCGCCCGGGGGTGGCGGGCCACCGccgcta                                                                      >  8:315600/1‑131 (MQ=255)                                                aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCtat                                                                       >  3:297492/1‑134 (MQ=255)                                                aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGGCGCCTGGGGGGGGCAGGCCCCCGccga                                                                        >  2:27961/1‑131 (MQ=255)                                                aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCAGCCAGGGGGTGGCGCGCCCACCCCgata                                                                      >  2:48587/1‑135 (MQ=255)                                                          aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCCACGCCGATAAGGCGCAGcg                                                            >  1:65995/1‑134 (MQ=255)                                                          aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGGTGGCCCCCCCCGATAAGGCGCAGcg                                                            >  6:241514/1‑134 (MQ=255)                                                                    gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCGCGGGCGGGGCCCCGGCGGCCGGGGGGTGGGGGGCCCACGCCGCTAAGGCGCAGCGCGCGGCTCAg                                                  >  4:6749/1‑135 (MQ=255)                                                                    gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCCGCCGGGGGGTGGCAGGCCCCCCCCGATAAGGCGCAGCGCGCTGATCAg                                                  >  7:116662/1‑135 (MQ=255)                                                                    gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGCGCGGCGCCCCGGGACCCGGGGTGGGGCAGCCCAACGCCTATAATCCGGCGCGCGCGGATCAg                                                  >  2:216987/1‑135 (MQ=255)                                                                      tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCCCGCAGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGCCCACGCCGATAAGGCGCAGCTCGCTGATCAGGc                                                >  4:237538/1‑135 (MQ=255)                                                                                 cAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGGGCGCGGGCACCCTGGGGGTGGCTGGCCCACGCCAATAAGGCGCCGCTCGCGGCTCAGGCCGCTGACGTCg                                     >  7:249637/1‑135 (MQ=255)                                                                                            tGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCCACCCCGAGAAGGCGCCGCGCGCGGCTCAGGCCGATGGCTGCGGCAAGCAGTTg                          >  7:179938/1‑135 (MQ=255)                                                                                                           gTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGCCAACGCCGATAAGGCGCAGCGCGCGGATCAGGCCGCTGACTTCGGGAAGCAGTTGGCGGAGAAGGATcgc           >  3:318501/1‑135 (MQ=255)                                                                                                             cGCCGGGGCGCGGCTGCCGGCCGGGCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc         <  1:168783/135‑1 (MQ=255)                                                                                                                  tGGCGCGGCGGCGGCCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACg    <  4:137609/135‑1 (MQ=255)                                                                                                                    gcgcGGCGGCTGCCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGt  <  1:203792/135‑1 (MQ=255)                                                                                                                                      |                                                                                                                     CGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGT  >  KT2440/3440210‑3440459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑