| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 434,376 | 0 | G | . | 21.7% | 58.7 / 13.9 | 23 | coding (207/1173 nt) | bioF | 8‑amino‑7‑oxononanoate synthase |
| Reads supporting (aligned to +/- strand): ref base G (12/6); new base . (5/0); total (17/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.19e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAAT > KT2440/434250‑434499 | gCAAGGGCCGGAAGTGGTGGTCGACGGCCCCCGGTTGCGGGGCGTGTGCAGCAATGACTAACGGGGCCTGGCCAACCACCCCGAGGTGATAGCCCCCTGGCAGGCCCGCGCCGAGCGCTGGGGTGTggggggggggg > 2:296929/1‑137 (MQ=255)gCAAGGGCCGGAAGTGGTGGTCGACCGCCCCCGGTTGGTGGGCATCTGGACCAATTAAAAACAGGGCCAGGGCAACCACCCCGCGGTGATCGCCCCCAGGCAGGCCCGGCCCCACCGCTgggggggggggggggggg > 4:171317/1‑137 (MQ=255) gAAGTGGTGGTCGACGGCCCGCGGTTTCTGGCCGTCTGGCGCAATGACAACCAGGGCCTGGCCCACCCCCCCCAGGGGCTAGCCCCCAGGCCAGCCCGCGCCCCCCGCGGGGGGGGGGGGGGGGGGGGCGCGccccg > 8:292732/1‑136 (MQ=255) tGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCCCAGCACCCCGCAcc > 2:182317/1‑137 (MQ=255) gCTGGCCGTCTCCACCAATCACTCCCGGGGCCGGGCCAACCACCCCGGGGTTTTGCCCGCCTGGCAGGCGGGCGCCGGGGGGGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAcc < 5:117623/137‑1 (MQ=255) agcaATTAATACCTGGGCCGGGCCAACCCCCCCGTGGGGATGGCCGGCGGGCAGGGCGGGGGCGTGCGGTGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAaga < 7:278486/137‑1 (MQ=255) aaattcaCTACCTGGGCCTGGCCAACCCCCCCGCGTTTCCCGGGGGGGGGCCGGCGGGGGGGGCGGGGGGGGGGTGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAagag < 2:16520/131‑1 (MQ=255) aCTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTTGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTgg > 2:260492/1‑137 (MQ=255) aCCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCCCCTGGTTGTTGGCCACCGCACCCCGCACCATCAGGTTGACGAGGCACTgggg > 7:214116/1‑135 (MQ=255) aCCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCCCCTGGTAGTTGGCCACAGCACCCCGCACCAGCAGGTTGAAGAGGCACTGCCTg > 6:371285/1‑137 (MQ=255) ggCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGTTCTTGGGCCACAGCACCCCGCCCCATCAGGTGGAAGAGCCCATGGCTGAACt > 5:30222/1‑137 (MQ=255) ggCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCCCCCCGCACCATCAGGTTGAAGCGGCACTGGCTGaaat > 1:303757/1‑135 (MQ=255) ccTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTTGTAGTTGGCCCCACCACCCCGCACCAACAGGTTGAAGAGGCACTGGCTGAAcaaac > 2:297012/1‑133 (MQ=255) aaCCACCCCGAGGTGATCGCCGCCTGGTAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCGCGCCCCGGGTGGTTGGCCACCCCCCCCCGCCCCTGCGGGTTGACGGGGCACTGGGTGAAGACACCGgccc > 7:342005/1‑136 (MQ=255) aaCCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACCGCACCCCGCACCATCAGGTTGAAGAGGCCCTGGCTGACCTCACCGgccg > 6:50053/1‑137 (MQ=255) ccccGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCCGGTTTATGAGGCACTGGGTGAACTCACCGGccgcccgc > 1:350180/1‑137 (MQ=255) aGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCCCCCCGCCCCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGccc > 1:264661/1‑137 (MQ=255) gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCAGCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCGggtgtt > 4:252953/1‑137 (MQ=255) ccTGGCAGGCCGGCGCCCTGGCGGGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGAGCGCCCTGTTGTTCTcc < 5:323664/137‑1 (MQ=255) cTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCa > 4:128554/1‑137 (MQ=255) ggCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCAGCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCCCCCTGTTGTTCTCCACCGGCTatat > 1:356830/1‑137 (MQ=255) cgcCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATgg > 5:398144/1‑137 (MQ=255) cGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCa > 7:11922/1‑137 (MQ=255) ggcgcGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAAt < 6:91178/136‑1 (MQ=255) cgcgcGGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAAt < 6:227197/136‑1 (MQ=255) | GCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAAT > KT2440/434250‑434499 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |