| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 690,162 | 0 | G | C | 52.6% | ‑0.9 / 12.4 | 19 | intergenic (+39/‑2420) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base G (6/3); new base C (10/0); total (16/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.67e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGG‑CCTCCCCGC‑TTTG‑T‑TTTTG‑GGGGTTGGTTGTTGCTGT‑TGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTC > KT2440/690028‑690293 | ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCCTGACG‑CCGCCCCGcg > 8:120232/1‑136 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGCCCGG‑CCGCCCCCG‑GTTG‑T‑TTTTg‑ggggg > 2:285819/1‑136 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCCCG‑GGTCCCCCCGGTTG‑T‑TTTTg‑ggggg > 4:172515/1‑136 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGACGCG‑CGCGCCCCC‑TTTG‑T‑TTTTG‑GGGTTGGggtgt > 2:46714/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGTCACG‑CCTCCCCGC‑GGTG‑T‑TTTTT‑GGGGTtgtttgtt > 8:294563/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCAGG‑GCGGCCCGC‑GTTG‑TGTTTTG‑GGGGTGGgttgt > 4:346179/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCAGG‑GCGGCCCCC‑GGTGTT‑TTTTT‑GGggttggtgtt > 3:312112/1‑134 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGCCGG‑CCCACCCGC‑GTTG‑T‑TTTTGTGGGggtggtggt > 8:5348/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCGGCGGGCCGGCCCCCCCCCC‑GGGG‑T‑TTTTG‑GGGggtggtggt > 7:165056/1‑137 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGAGG‑CCGCCCCGC‑GTTG‑T‑TTTTG‑GGGGGTGGgtgttgg > 6:182401/1‑136 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGGCCTCACG‑CCGCCCCCC‑ATTT‑A‑TTTTG‑GGGGTGTGtggttgg > 6:354146/1‑136 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGGGG‑GCGCCCCCC‑GGTG‑T‑TGTTT‑GGGGGTGGGTGTTTGTGT‑TGgggggggggtt > 2:248572/1‑132 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGCGG‑GCGCCCCGC‑GGTG‑T‑GTTTT‑GGGGGGGGGTGGTGGGGT‑TGgggggggaggt > 7:145848/1‑132 (MQ=255) ttGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGCCGGG‑CGCCCCCCG‑TTTT‑T‑TTTTG‑GGGGGT‑GTTGTTGGTGT‑TGGGGGGGGACGGt > 7:321568/1‑137 (MQ=255) ttGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGCCG‑CCCCCCCCC‑GTTGTT‑TTTTG‑GGGGTTGG‑TGTTGGGGT‑TGGGGGGGGACGGt > 7:283703/1‑137 (MQ=255) tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGGCGGAGG‑CCCCCCCCC‑CGTG‑T‑TTTTT‑GTGGTTGGTTTTTG‑TGTGTGGGGGGGGACTggt > 5:125775/1‑135 (MQ=255) cccccGC‑TTTG‑T‑TTTTG‑TGGGTTGGGTGGTGCGGT‑TGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGtctc < 4:131902/136‑1 (MQ=255) cccccGC‑TTTG‑T‑TTTTG‑GGGGTTGGTTGTTGCTGT‑GGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGACATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGAGCGAGCGCCGCCCGCGCGGCGCTCGGtctc < 8:80268/136‑1 (MQ=255) cccccGC‑TTTG‑T‑TTTTG‑GGGGTTGGTGGTTGCTGT‑TGGGGAGGAACTGTTTTGAGCGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGCGATCGAGCGCCGCCCGCGCGGCGCTCGGtctc < 6:218302/136‑1 (MQ=255) gC‑TTTG‑T‑TTTTG‑GGGGTTGGTTGTTGCTGT‑TGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGc > 4:288094/1‑77 (MQ=255) gC‑TTTG‑T‑TTTTG‑GGGGTTGGTTGTTGCTGT‑TGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGc < 3:288094/77‑1 (MQ=255) | GGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGG‑CCTCCCCGC‑TTTG‑T‑TTTTG‑GGGGTTGGTTGTTGCTGT‑TGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTC > KT2440/690028‑690293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |