Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,113,7580CG36.4% 40.7 / 11.3 36F49L (TTC→TTG) lptFLipopolysaccharide export system permeaseprotein LptF
Reads supporting (aligned to +/- strand):  ref base C (5/16);  new base G (12/0);  total (20/16)
Fisher's exact test for biased strand distribution p-value = 2.26e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGACCGTGCTGTCGGCCACTGGCAT  >  KT2440/1113626‑1113889
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ttATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGa                                                                                                                                 <  4:350015/137‑1 (MQ=255)
          cgcgAGGTCCTGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATTAGCGGGCGCTTCATAAAGTAACTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCCGGCGTGTTGTTGCTGATCATgtggtt                                                                                                                       >  7:273826/1‑132 (MQ=255)
               ggTCCTGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCt                                                                                                                  <  5:266703/137‑1 (MQ=255)
                    tGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTGATGTTGATTGGCGTGCGCTTGCCa                                                                                                             >  3:383524/1‑137 (MQ=255)
                             tGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTg                                                                                                    <  4:210429/137‑1 (MQ=255)
                              gAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGCCCCGGGCGTGTGGTGGGTGTTGAAGGGCGTACGCTTGGCCGGCGTccatg                                                                                                   >  2:311450/1‑134 (MQ=255)
                              gAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTGCTGATCATTGGCGTCCGCATGCCACGCTTCCTGc                                                                                                   >  7:51604/1‑137 (MQ=255)
                              gAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTGCGGCTGATGGGGGTGCGCTTGGCAGGGTTccggg                                                                                                   >  7:81779/1‑134 (MQ=255)
                              gAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTTTTTCTGATAATTGGGTTTCGCATGGCAAGCTAccggg                                                                                                   >  5:17465/1‑134 (MQ=255)
                                        aGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCt                                                                                         >  8:303208/1‑137 (MQ=255)
                                        aGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTGGGTGGTGATCATGGGGTTCCGCGTGGCCCGCGTGCGGGAACTGATTCt                                                                                         >  4:217012/1‑137 (MQ=255)
                                        aGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGTTGCTGAGCAAGGGCTTCCGCTTGCCCGGCGTCCTGGGACTGAttat                                                                                         >  3:309692/1‑135 (MQ=255)
                                          cgcTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGc                                                                                       <  2:166959/137‑1 (MQ=255)
                                          cgcTGTGCTGGTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGTGCTTCCGGTTGCCAGGCTTCCTGCAACTGATTCTGc                                                                                       <  4:81392/137‑1 (MQ=255)
                                               tgctgtTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTg                                                                                  <  6:326916/137‑1 (MQ=255)
                                                 ctgtTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGGTGCGGCTCATGGGCGTCCCCTTTCCAGGCGTGCTGCAACTGATTATGCCTCgggg                                                                                >  7:72039/1‑137 (MQ=255)
                                                  tgtTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGTTACTGATCATGGGGTTCCGCTTTCCAGGCTTCCTGCAACAGATTATTCCTCTgggg                                                                               >  3:190124/1‑137 (MQ=255)
                                                    ttGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCGTCCTGCAAATGATTCTGCCTATGGGGcg                                                                             >  6:236722/1‑136 (MQ=255)
                                                       gTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGCCCCGGGCGTGTTGTTCCTGATCATGGGCTTACGCTTGCCAGGCTTCCTGCAACTGATTCTGCATCTTGGGCTGtt                                                                          >  2:324416/1‑137 (MQ=255)
                                                       gTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTGCTGATCAATGGGTTACGCTTTCCAGGCGTGCAGGAAATGATTATTCCGATgggggggtt                                                                          >  3:312991/1‑132 (MQ=255)
                                                       gTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGtt                                                                          <  1:19713/137‑1 (MQ=255)
                                                       gTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGGGTGCCTGTTCATGGGGGGCCGCTTGGCGGGCGTCATGCACCGGGTTGGGCCTGGGGGGGTGtt                                                                          >  4:268874/1‑137 (MQ=255)
                                                           tcatcaTGAGCGGGCGCTTCATCAAGTACCTGGCGCAGGCTGCCCAGGGCGTGCTCGACCCGTGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTc                                                                      <  3:82086/137‑1 (MQ=255)
                                                           tcatcaTGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTc                                                                      <  7:303208/137‑1 (MQ=255)
                                                            catcatGAGCGGGCGCTTCATCTAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGTCCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCg                                                                     <  1:360294/137‑1 (MQ=255)
                                                            catcatGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTTCGGTTCATTGGCTTCCGCTTGGCACGCGTCCGGCAACTGATTATGCCTCGGGGGGGGGTCCTCg                                                                     >  2:339791/1‑137 (MQ=255)
                                                            catcatGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCg                                                                     <  4:173645/137‑1 (MQ=255)
                                                            catcatGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCg                                                                     <  3:69429/137‑1 (MQ=255)
                                                            catcatGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCCCTTGCCCGGCGTCCTGCAACTGATTATGCCGCTGGGGCTGTTCCTCg                                                                     >  6:78547/1‑137 (MQ=255)
                                                                                       ccTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGGTGATGATCATTGGGGTAAGGGTGCCCGGCGTCCTGGAACTGATTCAGCCGCTGGGGGGGGTGCTCGGCATACTCCGGGGTTACGGGGGCCTgg                                          >  4:1794/1‑136 (MQ=255)
                                                                                                  cTGCCCAGGGCGTGCTCGACCCGGGCGTGGTGTTGCTGATGATGGGGGTCCGCGTGGCAGGCGTCCTGCCACTGATTATGGCTCTGGGGGTGTTGATAGGCATGCGCCAGTCTTTCGGGCGCCGGTACCTGGGAAGc                               >  6:88986/1‑137 (MQ=255)
                                                                                                       cAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGAt                          <  3:236508/137‑1 (MQ=255)
                                                                                                           gCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGAcc                      <  7:128335/137‑1 (MQ=255)
                                                                                                                  cGACCCGGGCGTGTTGTTGCTGATCATGGGGTTCCCCTTGCCAGGCTTGCTGCAACTGATTCTTCCTCTGGGGGTGTTCCACGGCCTCCGCCAGGCTTACGGGCGCCGGTTCCGGGGAAGCGAGCTGAACGTGCTGt               >  8:356912/1‑137 (MQ=255)
                                                                                                                              gttgttCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGACCGTGCTGTCGGCCACTGGCa   <  1:339791/137‑1 (MQ=255)
                                                                                                                               ttgttCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGACCGTGCTGTCGGCCACTGGCAt  <  8:51604/137‑1 (MQ=255)
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TTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAGCGCTGTGCTGTTGGTGATCATCATGAGCGGGCGCTTCATCAAGTACCTGGCCCAGGCTGCCCAGGGCGTGCTCGACCCGGGCGTGTTGTTCCTGATCATGGGCTTCCGCTTGCCAGGCTTCCTGCAACTGATTCTGCCTCTGGGGCTGTTCCTCGGCATCCTCCTGGCTTACGGGCGCCTGTACCTGGAAAGCGAGATGACCGTGCTGTCGGCCACTGGCAT  >  KT2440/1113626‑1113889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑