| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,043,578 | 0 | A | C | 54.5% | 6.4 / 18.7 | 23 | intergenic (‑14/+161) | smrA/CJ019_01939 | putative DNA endonuclease SmrA/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/5); new base C (0/12); total (5/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.57e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCAGGCCGGCCAGCTTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGGATGTCACAGAGCCCGAAGACGGCGGGGTGCCT > KT2440/2043446‑2043688 | cgcAGGCCGGCCAGCTTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAggcg < 3:332768/137‑1 (MQ=255)cgcAGGCCGGCCAGCTTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAggcg < 6:65943/137‑1 (MQ=255) ggggcGGCGTTGGGCTTGCCGACTTCGGCACGGGCGGGGCGTAGCGGCTTTACGCCGCGCACTTCGGCCTTCAACAGGCAAAAATCGTCGTCTTGCATGATGCCCCCACGTCGGCGGCGTAGTTTCCGCGACTGTgg < 2:228824/133‑1 (MQ=255) ggggcGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGCAAAAATCGTCGTCTTGCATGATGCCTCCACGTCGGCGGCGTAGTTTACGCGACTGTgg < 7:44793/133‑1 (MQ=255) gggTCGGCGTGGGGCTTGCCGACTTCGGCACTGGGGGGGGGGCTCGGTTTAACCCCGCGCAGTTCGGCCTTCACCAGGAAAAAAGCGGCGTCTTGCATGATCCCTCACCGGCGGCGGCGTATTCCCCGCGACTGTgg < 2:325381/136‑1 (MQ=255) gggTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTgg < 6:199273/136‑1 (MQ=255) gggTCGGCCTTGGGCTTGCCGACTGCGCCACGGTCGGGGCGCATCGGCTTTCCGCCGCGCACTTCGGCCTTGAACCGGTAAAAATCGGCGTCTTGCAGGATGCCTCCACGTCGGCGGCGTAGTTTCCGCGACTGTgg < 6:70510/136‑1 (MQ=255) cGGGCGGCGTTGGGCTTGCCGACTTCGGCAGGGTCGGGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGCAAAAATCGTCGTCTTGCATGAGGCCTCCACGTCGGCGGCTTTGTTACCGCGACTGTgg < 8:13758/137‑1 (MQ=255) gACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGccctgtct > 5:220508/1‑131 (MQ=255) gACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCg > 4:53755/1‑137 (MQ=255) gACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCATGCATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCg > 6:193000/1‑137 (MQ=255) gACTTCGGCACGGTATTTGAATATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCg > 5:161463/1‑137 (MQ=255) gTCGGGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTCAACCGGCAAAAAGCGTCGTCTGCCATGATGCCCCCCCGTCGGGGGCGTAGTTTCCGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTcgcg < 5:370369/137‑1 (MQ=255) gggttCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGGCGTCTTGCATGATGCCTCCACGTCGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAAc < 2:256842/133‑1 (MQ=255) gggATCGGCTTTACGCCGCGCACTTCGGCCTTCAACAGGCAAAAATCGTCGTCTTGAATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAAc < 6:273496/136‑1 (MQ=255) cgtatgggCTTTACGCCGCGCACTTCGGCCTTGAACAGGTAAAAATCGGCGTCTTGCATGATGCCGCCACGTCGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAAc < 8:39857/131‑1 (MQ=255) gCTTTACGCCGCGCACTTGGGCCTTGAACTGGCAAAAATCGTCGTGTTGCAGGATGCAGCCACGTCGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTcc < 6:161463/137‑1 (MQ=38) tttACGCCGCGCAGTTCGGCCTTCACCAGGCAAAATGGGGCGTGTTGCATGATCCCTCCACGTCGGCGGCGTATTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTa < 2:35298/137‑1 (MQ=37) ttaccgccgcGCACTTCGGCCTTCAACTGGTAAAACTCGGCGTCTTGCATCATCCCTCCCCGTCGGCGGCGTAGTTTCCGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTa < 3:53755/133‑1 (MQ=37) cgccgcGCACTTCTGCCTTGAACAGGTAAAAATCGTCGTCTTGCATGTTGCCACCACGTTGGCGGGGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTACggg < 3:342797/137‑1 (MQ=38) aCTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCGgt < 7:211089/83‑1 (MQ=255) aCTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCGgt > 8:211089/1‑83 (MQ=255) cggcgacTTGAATCAGGCCTCCAGTTCGGCGGGGTAGTTACCGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCAAACGGGGATGTCACAGAGCCCGAAGACGGCGGGGTGCCt < 5:186854/131‑1 (MQ=37) | CGCAGGCCGGCCAGCTTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTACGCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGGATGTCACAGAGCCCGAAGACGGCGGGGTGCCT > KT2440/2043446‑2043688 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |