Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,308,3360CG28.9% 75.4 / 10.7 46A225P (GCA→CCA) fucAL‑fuculose phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (10/22);  new base G (13/0);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 2.25e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAG  >  KT2440/2308212‑2308468
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gagTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATggg                                                                                                                          <  7:338474/137‑1 (MQ=255)
cagTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGGACCGTGTCGTTTGGGGGTGGAACTCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATggg                                                                                                                          <  4:55179/136‑1 (MQ=255)
  gTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGCTTTTCCCAGTGCTTCGGGGCTtgggg                                                                                                                        >  4:60352/1‑136 (MQ=255)
    aCAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCGTTGGCCAGTGGTTAGGGGATGGGGCgg                                                                                                                      >  4:278987/1‑137 (MQ=255)
    aCAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGCTTTGCCCAGTGAGTACGGGCTGGGGCgg                                                                                                                      >  8:184304/1‑137 (MQ=255)
           cGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCAc                                                                                                               <  5:391277/137‑1 (MQ=255)
              tCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGCTTTGGCCAGTGGTTGGGGGGTGGGGGGGGTGTGACCgg                                                                                                            >  3:134554/1‑137 (MQ=255)
              tCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGGGTTGGCCAGGGGGTCGGGGCTGGGGGGGGTGTGCCCgg                                                                                                            >  3:62484/1‑137 (MQ=255)
                     aGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCTTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCAt                                                                                                     <  5:107109/137‑1 (MQ=255)
                      ggCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATg                                                                                                    <  3:250839/137‑1 (MQ=255)
                         aGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGCGTTGTCCCGTGGTTCGGGGGTGGGGCGGCGGGGACCGGCGccccggggc                                                                                                 >  3:221866/1‑131 (MQ=255)
                          gTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTCTTTGCCCCGGGGTTGGGGGATGGGGGGGATGTGACCGGCGCCCCTGGccc                                                                                                >  7:303760/1‑136 (MQ=255)
                              ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGGGTCGGGGATGGGGCGGCGGTCCCCCGCGCCCCAGGCcaccag                                                                                            >  8:304868/1‑137 (MQ=255)
                              ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGCTTTGGCCAGGGGTTCGGGGTTGGGGCGGCTGTCGCCGGCGCCCCAGGcccggcg                                                                                            >  3:384728/1‑132 (MQ=255)
                              ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGCTTTGCCCAGTGCTTCGGGGATGGGGGGGGTGTCACCGGCGCCCATGGCcagcag                                                                                            >  8:60320/1‑137 (MQ=255)
                              ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGCGTTTCCCAGTGGTGGGGGGGTGGGGGGGTTGTGACCGGCGCCCACTGCcagcag                                                                                            >  4:269046/1‑137 (MQ=255)
                               cATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCGGTGCGCACTGCGTGGGGGGTGGGGCGGCTGTCACCCGCCCCCCAGGCccgcacg                                                                                           >  2:107409/1‑135 (MQ=255)
                                         cAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTtg                                                                                 <  3:171026/137‑1 (MQ=255)
                                              cACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGAGGTTTGGGGGTGGACATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGTGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcg                                                                            <  4:122056/137‑1 (MQ=255)
                                              cACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcg                                                                            <  5:152488/137‑1 (MQ=255)
                                              cACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcg                                                                            <  4:353966/137‑1 (MQ=255)
                                                cGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGCTTTGGCCAGTGGTTGGGGGATGGGGGGGATGTCACCGGCGCCCCAGGCCAGCAGTTGCATAGTTTCTgcgcg                                                                          >  6:61938/1‑137 (MQ=255)
                                                 ggCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcgcgc                                                                         <  8:62248/137‑1 (MQ=255)
                                                 ggCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcgcgc                                                                         <  1:107409/137‑1 (MQ=255)
                                                 ggCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGACGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcgcgc                                                                         <  4:103991/137‑1 (MQ=255)
                                                  gcgcgcGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCt                                                                        <  6:340000/137‑1 (MQ=255)
                                                    gcgcgTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGCTGTCACCGGCGCCCATGGGCAGCAGTTGGATCTTTTCTGCGCGCTCg                                                                      >  5:156047/1‑137 (MQ=255)
                                                     cgcgTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGCGGTGGCCAGGGCTTGGGGGTTGGGGCGGCTGTCACCGGCGCCCAAGGCCACCAGGTGCATCTTTTATgcgcgggcgc                                                                     >  3:32606/1‑132 (MQ=255)
                                                                     gcagcaCCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGGGCGTTGGGCAGTGGGTGGGGGGTGGGGGGGGTGTCACCGGCGCCCCTGGGCAGCACGTGCATCTTTGCTGGGCGCGCGAACAGCCGAGCGCGCAc                                                     >  4:23700/1‑137 (MQ=255)
                                                                     gcagcaCCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGCTTTTGCCAGGGCTTGGGGGATGGGGCGGGTGTCACCGGCGCCCCAGGCCAGCAGGTGCATCATTGCTGAGCGGTCGAACAGCCgcgcgcgccc                                                     >  6:89411/1‑135 (MQ=255)
                                                                       agcaCCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGCTTTGGCCAGTGGTTCGGGGATGGGGCGGGTGTGACCGGCGCCCCAGGCCCGCAGTTGGATCTTTGCTGCGCGCGCGCACAGCAGAGCGCGCACGc                                                   >  5:341587/1‑137 (MQ=255)
                                                                       agcaCCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGCGTTGCCCACTGGGTCGGGGGTGGGGCGGATGTGACCGGCGCCCCTGGCCAGGCGTTGGATCTTTGCTTCGCGGGCGCACACCACCGCGAGCCCGc                                                   >  8:116332/1‑137 (MQ=255)
                                                                        gcaCCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGGGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCa                                                  <  1:114403/137‑1 (MQ=255)
                                                                            cGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGGCCAGTGCTTCGGGGATTGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTTCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCCGGCt                                              >  5:180908/1‑137 (MQ=255)
                                                                            cGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGgcg                                                                                                          >  2:67009/1‑77 (MQ=255)
                                                                            cGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGgcg                                                                                                          <  1:67009/77‑1 (MQ=255)
                                                                            cGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGCTTTTGCCCGTGGTTCGGGGCTGGGGCGGATGTCACCGGCGCCCATGGCCCGCAGTTGCATCTTTTCTGCGCGCTCGAACCGCAGAGCGAGCACGCCGGcg                                              >  5:134112/1‑136 (MQ=255)
                                                                             gTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCtt                                             <  3:60352/137‑1 (MQ=255)
                                                                                              aaaTCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCa                            >  7:245748/1‑137 (MQ=255)
                                                                                                   cAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGAt                       <  2:126357/137‑1 (MQ=255)
                                                                                                     gTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATca                     <  8:245748/137‑1 (MQ=255)
                                                                                                        gTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATcagca                  <  7:304868/137‑1 (MQ=255)
                                                                                                                ctTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCatga          <  6:370044/137‑1 (MQ=255)
                                                                                                                ctTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCatga          <  4:350484/137‑1 (MQ=255)
                                                                                                                   tGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATgg       >  3:164747/1‑137 (MQ=255)
                                                                                                                        tgTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAg  <  4:2372/136‑1 (MQ=255)
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GAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAG  >  KT2440/2308212‑2308468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑