Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,026,5900AC40.0% 24.7 / 13.2 35Q19P (CAG→CCG) CJ019_02841hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (9/12);  new base C (14/0);  total (23/12)
Fisher's exact test for biased strand distribution p-value = 6.13e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGC  >  KT2440/3026456‑3026724
                                                                                                                                      |                                                                                                                                       
cGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCCAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCGGCCGCCCgg                                                                                                                                       >  2:275084/1‑137 (MQ=255)
cGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTTCGCCGACACCCGGCGCTGCCCCCCgg                                                                                                                                       >  5:145460/1‑137 (MQ=255)
cGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGGCGCCAgg                                                                                                                                       >  5:346293/1‑137 (MQ=255)
  tGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCACGcc                                                                                                                                     >  8:321169/1‑137 (MQ=255)
     tCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTg                                                                                                                                  >  8:280472/1‑137 (MQ=255)
     tCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTg                                                                                                                                  >  4:297988/1‑137 (MQ=255)
          cAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCCGCCCGGgaccc                                                                                                                             >  5:270592/1‑135 (MQ=255)
                aTGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTcca                                                                                                                       >  8:271863/1‑137 (MQ=255)
                          tctATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCGGCCCTGGACGACGTCCAGCGCCACCgg                                                                                                             >  6:167443/1‑137 (MQ=255)
                              tatCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCTAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGGGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGa                                                                                                         <  7:90047/137‑1 (MQ=255)
                              tatCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGa                                                                                                         <  6:231495/137‑1 (MQ=255)
                                 ccgccgTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGAT‑cc                                                                                                     <  3:297988/137‑1 (MQ=255)
                                     cgTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCCGCCCGGGACGCCGTCCAGCGCCCGCGGCCGAT‑CCgggg                                                                                                 >  4:243025/1‑136 (MQ=255)
                                       tttCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCCGCCCTGGACGACCTCCCCCGCCCGCGGGCGATACCggggg                                                                                                >  6:280714/1‑135 (MQ=255)
                                       tttCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCCGCCCAGGTCGACGGCCCCCGCCACCGGCCCGT‑CCgggggg                                                                                               >  5:210495/1‑134 (MQ=255)
                                       tttCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCAGGACGACGTCCAGCGCCAGCGGCCGAT‑CCCGgggt                                                                                               >  2:60166/1‑137 (MQ=255)
                                                    ttCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGGCCCCCCGCCCAGGACGCCGGCCCGCGCCCGCGGGCGGT‑ACGGGGGTTTGTGCACCGTgg                                                                                  >  4:88594/1‑137 (MQ=255)
                                                                          aGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCGGCCCAGGCCGCCCTCCAGCGCCAGCGGCCGCT‑CCGGGGGTTGCTCCACGGTGGCAGCCAGTTTCTTGGGGGGGaa                                                            >  6:377567/1‑137 (MQ=255)
                                                                               aTGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCCCCCCGCCCCGGTCGCCCTGCACCGCCCCCCGCCGCT‑GCCGGGGGTGGTCCACCGTGGGAGCCCGGTTGTTGGGGGGGaaaaaga                                                       >  4:326056/1‑133 (MQ=255)
                                                                                    aGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCCCCCGGCCCTGGCCGCCCGCCAGCGCCACCGGCCCCA‑CCGGGTGTTGATACCCGGTGGCACCCAGTTTCTTGGGGGGGTAATCGAAAGCt                                                  >  7:321344/1‑137 (MQ=255)
                                                                                        cTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGgc                                              <  6:117157/137‑1 (MQ=255)
                                                                                              tgtgCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGa                                                                                                         <  1:276636/73‑1 (MQ=255)
                                                                                              tgtgCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGa                                                                                                         >  2:276636/1‑73 (MQ=255)
                                                                                              tgtgCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGa                                        <  8:297481/137‑1 (MQ=255)
                                                                                              tgtgCAGGGCGCAACTACGCCGACACCCGGCGCTGCCCCCCCGCCCGGGACGCCGGCCCGCGCCCCCGGCCGGT‑CCGGGGGGTGGTGCCCGGTGGCGGCCCGGTTGTTTGTGGGGTAATAGAAAGCTGGGggcgcgc                                        >  2:35769/1‑136 (MQ=255)
                                                                                                   aGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATg                                   <  5:256815/137‑1 (MQ=255)
                                                                                                     ggCGCAACTACGCCGACACCCGGCGCTGGCCCCCGGCCCGGGGCGCCGGGCCGCCCCCCCGGCCGAT‑GCGGGGGTTTGTACCCGGTGGCCGCCAGTTTGTTTGTGGGGAAATGGAAATCTGGGCGCGCGGGCCTggg                                 >  4:375416/1‑136 (MQ=255)
                                                                                                        gcAACTACGCCGACACCCGGCGCTGCCCCCACGCCCGGGGCGCCGTCCAGCGCCCGCGGCCGCT‑CCGGGTGGTGCTCCACGGTGGCAGCCAGTTTCTTGGGGGGGAAATCGAAAGCTGGGCGCGCGCGCATGGCGcc                              >  7:297481/1‑137 (MQ=255)
                                                                                                          aaCTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGGCCAGCGCCTGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGa                            <  8:234438/137‑1 (MQ=255)
                                                                                                           aCTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGAt                           <  6:270592/137‑1 (MQ=255)
                                                                                                             tACGCCGACACCCGGCGCTGCCGCCCCGCCCGGGACGACCTCCACCGCCAGCGGCCGCT‑CCGGGTGTTGTTACACGGTGGCCGCCAGTTTGTTGGGGGGGCAATCGAAAGCTGGGCGCGCCCGCATGGCGCCGctct                         >  6:267584/1‑137 (MQ=255)
                                                                                                                  cGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGGGTTGATCCACGGTGGCAGCCAGGTTCTTGGTGGGGGAATAGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTgg                    >  7:290483/1‑136 (MQ=255)
                                                                                                                                gccgccAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAAc      <  4:47242/137‑1 (MQ=255)
                                                                                                                                 ccgccAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACt     <  6:378680/137‑1 (MQ=255)
                                                                                                                                    ccAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGc  <  3:157534/137‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGC  >  KT2440/3026456‑3026724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑