Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,273,8950CA24.5% 100.6 / 11.9 53D40E (GAC→GAA) fabG_53‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG
Reads supporting (aligned to +/- strand):  ref base C (20/20);  new base A (0/13);  total (20/33)
Fisher's exact test for biased strand distribution p-value = 7.92e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGATTGAGGCA  >  KT2440/3273766‑3274030
                                                                                                                                 |                                                                                                                                       
ggAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGc                                                                                                                                  <  8:130071/137‑1 (MQ=255)
 gAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCa                                                                                                                                 <  5:181489/137‑1 (MQ=255)
 gAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCa                                                                                                                                 >  2:46071/1‑137 (MQ=255)
  cggcaggcaTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAg                                                                                                                                <  6:92076/136‑1 (MQ=255)
  agtcaggcaTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGGTATATTGCCTGCGTAAGCCGTCGCCTGGCCGACATACTGCAg                                                                                                                                <  6:93743/134‑1 (MQ=255)
  aggcaggcATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAg                                                                                                                                >  6:12383/1‑137 (MQ=255)
     caggcaTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCg                                                                                                                             <  8:146728/137‑1 (MQ=255)
           tGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGctgctg                                                                                                                       <  5:371669/137‑1 (MQ=255)
              cAGAACAACAAAAAGTGGTGGTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGTGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCTTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTggag                                                                                                                    <  5:173635/137‑1 (MQ=255)
                         aaaGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACAcgc                                                                                                         >  2:228169/1‑137 (MQ=255)
                         aaaGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACAcgc                                                                                                         >  4:374187/1‑137 (MQ=255)
                           cgTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCATGCGTTTTGCGCGTGAGGGCAATATTGCCTGCGTACCCCGTCGCCAGGCCGACAAACTGCCGCCGCTGCTGGAGGAAAAACAcgccg                                                                                                       <  4:46361/136‑1 (MQ=255)
                                tGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGAGCGCCAAGCGTTTTGCGCGTGCGGGATATATGGCCTGCGTACCCCGTCCCCAGCCCGACAAACTGCCGCCGCTGCTGGAGTAAAACCACGCCGCTggc                                                                                                  <  4:95846/137‑1 (MQ=255)
                                ggggggTGAGCGGGGCGGGCGACGCCACGGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGATATATTGCCGGCGAAACCCGGCGCCAGCCCTAAAAACTGCCGCCGCTGCGGGAGCAAATCCACGCCGCTggc                                                                                                  <  4:39232/133‑1 (MQ=255)
                                ggCTGGGGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGGGCGTGAGGGCTATATGGCCGGCGTAACCCGGCCCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTggc                                                                                                  <  2:216469/136‑1 (MQ=255)
                                 gCTGGGGAGCGGGGCGGGCGACGCCCCGGGCGGCGAGAGCCCCAAGCGTTTGGCGCGTGAGGGCTATATTGCCTGGTAAACCCGCCGCCAGGCGGACAAACTGCCGCCGGGGCGGGAGTAAAAACACGCCGCTggcg                                                                                                 <  4:384556/137‑1 (MQ=255)
                                              gggggcgACGCCTCTGGGGGCGATATCGCAATGGTTTTTGGGCGTGTGGTTTATATTGCGGGCGTAACCCCTCGCGTCGCCTAAAAACTTCAGCCGGTTGGGGTGTTAATACACGCCGCTGGCGGCCTGGCCTACgg                                                                                    <  4:238981/135‑1 (MQ=255)
                                                gggcgACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACggct                                                                                  >  1:93447/1‑135 (MQ=255)
                                                    gACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTgg                                                                              >  2:25738/1‑137 (MQ=255)
                                                           cTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGAAACCCGCCGCCTGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGAt                                                                       <  6:388148/137‑1 (MQ=255)
                                                             gtcggggAGATCGCCATTGGTTTTGCGCGGGAGGGCTATTTTGCGTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTTGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGTCTCTGATgc                                                                     <  2:39450/135‑1 (MQ=255)
                                                             ggcggcGAGATCGCCAAGCGTTTTGCGCGGGAGGGCTATATTGCCTGCTTACCCCGTCGCCAGGCCGAAAAACTCCCGCCGCTGCTGGAGGAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATgc                                                                     <  2:153319/137‑1 (MQ=255)
                                                             ggaggcgATATCGCAAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCTTCGCCAGGCCTAAAAACTGCAGCCGCTGCGGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATgc                                                                     <  8:283438/134‑1 (MQ=255)
                                                                         gCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAaga                                                         >  7:221547/1‑137 (MQ=255)
                                                                           cAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAgagg                                                       >  1:321071/1‑137 (MQ=255)
                                                                               ggTTTTGCGCGTGTGGGCTTTTTGGCCGGCTTAACCGGGCGCCTGGCCGAAAAACTGCAGCGGCTGCTGGTGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGt                                                   <  5:369221/136‑1 (MQ=255)
                                                                                   ttGCGGGTGAGGGATATATTGCCTGCGTAACCCTGCGCCAGGCCTAAAAACTGCAGCCGCTGCTGGAGAAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                               <  5:12383/137‑1 (MQ=255)
                                                                                   ttGCGCGTGCGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                               <  2:11731/137‑1 (MQ=255)
                                                                                   tggcgcGTGGGGCCTATATGGCCTGCCACACCGGCCGCCCGCCCTAAAAACTCCCGCCGCTGGGGTAGAAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                               <  5:104430/135‑1 (MQ=255)
                                                                                   gtGCGCGTGAGGGCTATATTGCCTGCGTACCCCGTCGCCCGGCCGACAAACTGCCGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                               <  8:182272/136‑1 (MQ=255)
                                                                                        ggTGCGGGCTTTATTGCCTGCGTACCCCGTCGCCCGGCCGACAAACTCCCGCCGCTGGTGGAGTAAATCCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACt                                          <  3:338474/136‑1 (MQ=255)
                                                                                            aGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTa                                      >  5:105989/1‑137 (MQ=255)
                                                                                                tttttttGCGTGCGTACCCCGGCGCGTGGCCTAAAAACGGCAGGGGGTGGGGTAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAgaga                                  <  4:125209/132‑1 (MQ=255)
                                                                                                  attttGCCTGCCAAACCCGGCGCCTGCCCTAAAAACTCCAGCCGCTGCTGGAGAAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACt                                <  1:46071/134‑1 (MQ=255)
                                                                                                      tGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCg                            >  5:204606/1‑137 (MQ=255)
                                                                                                       gCCTCCGTAACCCGGCCCCCGGCCTAAAAACTCCAGCCGCTGGTGGAGGAAATCCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa                           <  2:321071/137‑1 (MQ=255)
                                                                                                           gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAagag                                                        >  7:233009/1‑104 (MQ=255)
                                                                                                           gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGt                       >  5:227131/1‑137 (MQ=255)
                                                                                                           gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGt                       >  1:315339/1‑137 (MQ=255)
                                                                                                           gCGTAACCCGTCGCCAGGCCGAAAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAagag                                                        <  8:233009/104‑1 (MQ=255)
                                                                                                             gTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGa                     >  5:165978/1‑137 (MQ=255)
                                                                                                              tAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGat                    >  6:109823/1‑137 (MQ=255)
                                                                                                               aaCCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGata                   >  3:376602/1‑137 (MQ=255)
                                                                                                                ccccGCCGCCAGGCCCAAAAACTGCCGCCGCTGGTGTAGCAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGatat                  <  1:70830/136‑1 (MQ=255)
                                                                                                                 ccggTCGCCTGGCAGACAAACTGCCGCCGCTGCGGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGAtata                 <  8:180523/134‑1 (MQ=255)
                                                                                                                      cGCCAGCCCGACAAACTGCAGCCGCTGCGGGAGCAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTcc            <  8:221547/137‑1 (MQ=255)
                                                                                                                        ccAGGCCGACAAACTGCAGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGa          <  5:169834/137‑1 (MQ=255)
                                                                                                                        ccAGCCCTAAAAACTCCAGCCGCTGCTGTTGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGa          <  5:341797/137‑1 (MQ=255)
                                                                                                                         ccggCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATAGAGGTCCGAt         >  5:56184/3‑137 (MQ=255)
                                                                                                                         cAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGAt         >  8:327177/1‑137 (MQ=255)
                                                                                                                         cAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGAt         >  7:29625/1‑137 (MQ=255)
                                                                                                                               gACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGATTGAGGc   >  2:235447/1‑137 (MQ=255)
                                                                                                                                aCAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGATTGAGGCa  <  2:5572/137‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                       
GGAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGATTGAGGCA  >  KT2440/3273766‑3274030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑