Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,427,1180GC31.0% 39.9 / 13.4 29intergenic (‑29/‑89)CJ019_04200/CJ019_04201hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (18/2);  new base C (0/9);  total (18/11)
Fisher's exact test for biased strand distribution p-value = 5.49e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCC  >  KT2440/4426991‑4427237
                                                                                                                                |                                                                                                                       
gAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTa                                                                                                                >  1:98762/1‑137 (MQ=255)
gAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTa                                                                                                                >  2:38076/1‑137 (MQ=255)
gAGCTGCGCAGTCGGCTTGCCGCCACGCGCAGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCATCACCGAAGCCAACGGCAGCATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTa                                                                                                                >  8:255843/1‑137 (MQ=255)
 aGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAg                                                                                                               >  3:186335/1‑137 (MQ=255)
      cgcAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCACCCAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGAAAAAAACAAGGCGACACTAGCACTg                                                                                                          <  7:388956/137‑1 (MQ=255)
         tgtgggCTGGCCGCCCCGCGCCGTTACATTGGTCGCACAACCAAACCCGCCCAGCAGCCCCCCCCCCCCCACCGGCAGAAGCCCACGATTGTG‑CCAACCTCGGGGGCCCAAAAAACACCGCGACTCTAGCACTggca                                                                                                       <  8:68207/133‑1 (MQ=255)
                   ccgccACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGa                                                                                             >  2:70768/1‑137 (MQ=255)
                     gccACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGt                                                                                           >  4:256075/1‑137 (MQ=255)
                        aCGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGaa                                                                                        >  6:69738/1‑137 (MQ=255)
                           cgcgGTTGCCTTGGCCGCAGACCCACACCCGCCCCGCACCACCACCCCCCCCACCGGCAGAAACCCCCCCATGTG‑CCATCCTCGGGGCCCAAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTg                                                                                     <  2:92887/137‑1 (MQ=255)
                                   ccATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCAc                                                                             >  6:103170/1‑137 (MQ=255)
                                           gCAGAAGCAAAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATCTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGAAGGCATCCCGAGTGAATAGCCGCGCACTTGCCGca                                                                     >  4:118477/1‑136 (MQ=255)
                                             aGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGt                                                                   >  3:288459/1‑137 (MQ=255)
                                                acaacacCCGCCCAGCACCCCCACCCCACCCACCGACAGAATCCCACGCATGTC‑CCCCCCGCGGGTTCCCAAAAACCACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGc                                                                <  3:83092/134‑1 (MQ=255)
                                                       ccGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATaaa                                                         >  1:90670/1‑137 (MQ=255)
                                                                      cccGCACCCACCGGAAAAATCTCACGC‑TGTTCCCATCGTGGGGGTCCAAAAAATGAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAg                                          <  4:259224/136‑1 (MQ=255)
                                                                        cccAGCAAACGCAAGAATAGCACGGATTTT‑CCATCATGGGTGTCTAAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGa                                        <  7:154944/135‑1 (MQ=255)
                                                                             ccACCGGAACAACCGCACGCTTGTT‑CCATCCTCGGGGGCCCAAAAAAGACCGCGACTCTAGCACTGGGAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCca                                   <  4:317589/137‑1 (MQ=255)
                                                                             ccAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCAATTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCca                                   >  8:239123/1‑137 (MQ=255)
                                                                                  ggCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGAATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGACCCCATCATg                              >  8:219291/1‑137 (MQ=255)
                                                                                     ataaTCGACCGCTTGTT‑CCACCCTCGGGGTCCAAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGaaa                           <  7:151976/135‑1 (MQ=255)
                                                                                       actCGCACGCATGTT‑CCATCCTGGGGGTCGAAAAAAAGACGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCTCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                         <  7:255843/135‑1 (MQ=255)
                                                                                       aaTCCCACGCATGTT‑CCATCCTCGGGGTCCAAAAAACCAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                         <  3:161692/137‑1 (MQ=255)
                                                                                       aaTCCCACGCATGTT‑CAATCCTCGGGGTCGAAAAAAAGAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                         <  6:367745/137‑1 (MQ=255)
                                                                                                             tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc    >  5:345336/1‑137 (MQ=255)
                                                                                                             tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc    >  6:164910/1‑137 (MQ=255)
                                                                                                             tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc    >  8:134029/1‑137 (MQ=255)
                                                                                                             tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc    >  4:192969/1‑137 (MQ=255)
                                                                                                               ggggTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGccc  >  6:384975/1‑137 (MQ=255)
                                                                                                                                |                                                                                                                       
GAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTT‑CCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCC  >  KT2440/4426991‑4427237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑