Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,384,2650CG33.3% 64.7 / 11.6 39intergenic (+528/‑330)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (4/22);  new base G (13/0);  total (17/22)
Fisher's exact test for biased strand distribution p-value = 2.93e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCT  >  KT2440/5384132‑5384395
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tGCCCGGCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCttt                                                                                                                                  <  2:13593/137‑1 (MQ=255)
 gCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCTGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCgttt                                                                                                                                 >  7:236071/1‑137 (MQ=255)
 gCCCGGCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGAAAGCGGATTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCtttt                                                                                                                                 <  2:72004/137‑1 (MQ=255)
   ccGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTTCCGACAGCGGAGTACTGGCGGACGGGGGTTAAAATCCCACCGGCTCCACCAAATCCAAAGAAGAAGACGCCCACGGCGGTGtttttt                                                                                                                               >  5:134670/1‑137 (MQ=255)
   ccGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGgttttt                                                                                                                               >  8:269333/1‑137 (MQ=255)
      tCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGAAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTtgt                                                                                                                            <  3:327868/137‑1 (MQ=255)
      gcGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTtgt                                                                                                                            <  7:115440/136‑1 (MQ=255)
              cTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGaa                                                                                                                   <  5:399960/137‑1 (MQ=255)
                                 acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGGTTTTTTTGCCT‑GGCATTTTGGGGGGGCggggggg                                                                                                >  5:224060/1‑133 (MQ=255)
                                    gggCTATGAATGTAGTACCGACTGCGGAGTTCTGGAGGACGGGGTTGCAAATCCCCCCGGCTCCACCTAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGaaaa                                                                                             <  4:264594/136‑1 (MQ=255)
                                        tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGCCG‑GGAATTTGGGGGGGGGGGGTCGAAAAATCg                                                                                         >  6:83146/1‑137 (MQ=255)
                                           gCATGTAGTACCGACAGCGGAGTACTGGCGGCCGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAACACGCCCTCGGGCGTGGTTTTTTGTTGCG‑GGTATTTTGGTGCCGCGGGGGGaaaaaaagaat                                                                                      >  4:32160/1‑131 (MQ=255)
                                             aTGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTTTTCCG‑CGAATATTGGCGCTGGGGGTCGCAAAATAGGAttt                                                                                    >  6:310763/1‑137 (MQ=255)
                                               gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                  <  1:339470/137‑1 (MQ=255)
                                                  gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCT‑GGAATTTTGGCGCGGAGGGGCGTAAAAACGGATTTAAacc                                                                               >  6:210394/1‑137 (MQ=255)
                                                   taCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcccc                                                                              <  2:272238/137‑1 (MQ=255)
                                                    aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTTGGCGGTGCGGGGCGAAAAATCGGCTTTTAacccg                                                                             >  6:376405/1‑136 (MQ=255)
                                                         cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTGTTTTTTGGCTGGGATTTTGGGGGGGGGGGGGGGTAAAAGCGGTTTTAACCGCtgctg                                                                         >  4:126670/1‑133 (MQ=255)
                                                           gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTTTGGCT‑GGAATTTTGGGGGGGGGGGGGGAAAAAAAGGATTTTAACCCTTtgttgt                                                                      >  5:66602/1‑137 (MQ=255)
                                                           gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTGTGGCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGt                                                                      >  6:358940/1‑137 (MQ=255)
                                                               aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGGT‑GGGGTTTGGGGGCGGCGGGGGGGAAAAAAGGAGTTTACCCCGTTGTTGTTggg                                                                  >  7:44170/1‑137 (MQ=255)
                                                               aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAggg                                                                  <  6:202129/137‑1 (MQ=255)
                                                                ttACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGc                                                                 <  1:354473/136‑1 (MQ=255)
                                                                 tACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCg                                                                <  2:148902/137‑1 (MQ=255)
                                                                     ggCGGACTGGGGTTCAAATCCCTCCGGCTCCACCAAATCCAAATAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                            <  8:74102/137‑1 (MQ=255)
                                                                     ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                            <  8:251603/137‑1 (MQ=255)
                                                                      gCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTcc                                                           <  5:363109/137‑1 (MQ=255)
                                                                         gACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTTTGGGG‑GGGATTTTGGGGCTGGGGGGGGGAAAATAGGATTTTACCCCCTTGTTGTTGGGGGTTTGCCaa                                                        >  6:31053/1‑137 (MQ=255)
                                                                           cGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAg                                                      <  5:83146/137‑1 (MQ=255)
                                                                            gggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCG‑GGGATTTGGGGGGGGCGGGGGGAAAAATAGGTTTTTAACCCTTTGTTGTTGGGGGGTTGCCCAAGa                                                     >  6:135273/1‑137 (MQ=255)
                                                                                       tCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTgcg                                          <  6:17938/137‑1 (MQ=255)
                                                                                           cccGGCTCCACCAAATCCAAAGAAGAAGATGCCCTCGGGCGTGGTTTTTTGTGGCT‑GGAATTTGGGCGCTGCGGGTGGAAAAATAGGATTTAACCCCTTTCTTTTAGGGCGGTTGCCAAAGATACTGCCGGCGccgg                                      >  1:208335/1‑135 (MQ=255)
                                                                                             cGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTGTGGGT‑GGGATTTTGGGGGTGGGGGGGGTAAAATAGGATTTTACACCGTTGTTTTTTGGGGTTTGGCAAAGATTATGCCTGGGGCTggt                                    >  4:107893/1‑135 (MQ=255)
                                                                                                        aaTCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCTGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                         <  8:161314/137‑1 (MQ=255)
                                                                                                              aagaagaagACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttt                   <  4:236569/137‑1 (MQ=255)
                                                                                                                gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                 <  2:354606/137‑1 (MQ=255)
                                                                                                                gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                 <  5:310763/137‑1 (MQ=255)
                                                                                                                              cGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtc   <  6:89864/137‑1 (MQ=255)
                                                                                                                               gggCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCGCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct  <  3:32160/137‑1 (MQ=255)
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TGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCT  >  KT2440/5384132‑5384395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑