Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,063,9950AC35.4% 76.7 / 13.4 48V162G (GTG→GGG) cheB_5Protein‑glutamatemethylesterase/protein‑glutamine glutaminase
Reads supporting (aligned to +/- strand):  ref base A (4/27);  new base C (17/0);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 1.41e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTT  >  KT2440/6063863‑6064123
                                                                                                                                    |                                                                                                                                
accGCCTTGATCACCCATGGCTCCATGCCACTGGCCCGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCtt                                                                                                                              <  4:9235/135‑1 (MQ=255)
aaCGCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCtt                                                                                                                              <  8:267277/137‑1 (MQ=255)
aaCGCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCtt                                                                                                                              <  3:24990/137‑1 (MQ=255)
   gCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCGTGAc                                                                                                                           >  5:159258/1‑137 (MQ=255)
   gCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGGGGGCGCCGCCCCCATgcc                                                                                                                           >  5:281313/1‑135 (MQ=255)
    ccTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGGGGCGGCGCCGCCCCCATGAcc                                                                                                                          >  1:35176/1‑137 (MQ=255)
         aTCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTc                                                                                                                     <  8:228506/137‑1 (MQ=255)
         aTCACACATGGCTGCAGGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGTAGAATCCACCTTGACCGCTTc                                                                                                                     <  5:88486/137‑1 (MQ=255)
               caTGGCTGGATGCCACTGTCCAGGCGCAGCTGGCCGTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCTGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCtt                                                                                                               <  8:252737/137‑1 (MQ=255)
                aTGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCTTGACCGCTTCGGGCTTg                                                                                                              >  6:376766/1‑137 (MQ=255)
                 tGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCACCGCCACCTTGACCGCTTCGGGCTTGc                                                                                                             >  4:235284/1‑137 (MQ=255)
                   gCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCATGCCCCCTTCGGGCGTGccc                                                                                                           >  7:82425/1‑137 (MQ=255)
                        aTGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTGGGCGCCGCGCCCCTGACCGCTTCGCGCTTgcgcgccgg                                                                                                      >  3:42963/1‑134 (MQ=255)
                          gCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGGGGGCGCCGCCCCCGAGACCGCTTCGGGCGTGGCCGcccgcc                                                                                                    >  1:372980/1‑132 (MQ=255)
                               tgcccAGGCGCAGCTGGCCCTGGCCACGTACTCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCCAAGGCTTGGCCGGTGCCGGTGTCGCCGCCGCCCCCCTGACCCCGACCGGCGTGACCGCAGTCAgcgcc                                                                                               >  6:325408/4‑136 (MQ=255)
                                 gCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCCCAGTCAGcgcggt                                                                                             >  6:172905/1‑135 (MQ=255)
                                    aGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCaa                                                                                          <  4:247140/137‑1 (MQ=255)
                                        gcAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAg                                                                                      <  4:354595/137‑1 (MQ=255)
                                         cAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGGCGGCGCCGCCCCCGTGCCCGCGTCGGGCGTGCCCGCCGGCCGCGCGGTGAACGAgg                                                                                     >  8:29833/1‑136 (MQ=255)
                                         cAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGGGTCGGCGCCGCCCCCGTGCCCGCGTCGGGCGTGCCCGCAGTCAGCGCGTGCAACGAgg                                                                                     >  4:254413/1‑136 (MQ=255)
                                              ggccctggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCTAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTg                                                                                <  5:325408/137‑1 (MQ=255)
                                              ggccctggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTg                                                                                <  3:254413/137‑1 (MQ=255)
                                               gcccgggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTgg                                                                               <  7:136676/137‑1 (MQ=255)
                                                       cACGGCCGCTCGGCGCGTGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGcc                                                                       <  7:128930/137‑1 (MQ=255)
                                                       cACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCGTGACCGCGTCGGGCTTGGCCGCAGGGCGCGGGGTCCACGAGGGGGTGGGAAAGGcc                                                                       >  2:92676/1‑137 (MQ=255)
                                                        aCGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCCCGTCGGGCTTGGCCGCAGTCAGCGCGTTGACAGAGTGGTTGGGAAAGGCCg                                                                      >  8:328767/1‑137 (MQ=255)
                                                        aCGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGGCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCg                                                                      <  8:343939/137‑1 (MQ=255)
                                                             cGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTGGGCGCCGCCCCCATGACCGCGTCGGGCTTGGCCGCCGGGCGCGCGGTGAACGCGGCGTTGGGGAAGGCCGGGGTg                                                                 >  4:76330/1‑137 (MQ=255)
                                                             cGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTg                                                                 <  6:31017/137‑1 (MQ=255)
                                                             cGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGGGTGGGCGGCGCCCCCCTGACCGGTTCGGGCGTGCCCCCCGTCAGCGCGTTGGACGGGGCGTTGGGAAAGGCCggggtt                                                                 >  4:380354/1‑134 (MQ=255)
                                                                  ggCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGTGCCGCCACCTTGACCGCATCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCtac                                                            >  5:367236/1‑135 (MQ=255)
                                                                  ggCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCGTGACCGCTTCGGGCGTGCCCGCAGGCCGCGCGGTGCACGAGTCGGTGGCAAAGGCCGGGGTGGCTcg                                                            >  5:90784/1‑137 (MQ=255)
                                                                  ggCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGGGTCGGCGCCGCCCCCGTGACCGCGTCGGGCTTGCCCGCAGTCAGCGCGGTCAACGAGGCGTTGGCGAAGGCCGGGTTGGGTcg                                                            >  7:130230/1‑137 (MQ=255)
                                                                           gcggcAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGcc                                                   <  6:326650/137‑1 (MQ=255)
                                                                           gcggcAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGcc                                                   <  8:297045/137‑1 (MQ=255)
                                                                           gcggcAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGcc                                                   <  4:366516/137‑1 (MQ=255)
                                                                                     cGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATc                                         <  2:207927/137‑1 (MQ=255)
                                                                                         cgccgcGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGACCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGc                                     <  7:331554/137‑1 (MQ=255)
                                                                                             gcgACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTc                                 <  1:222826/137‑1 (MQ=255)
                                                                                               gACCAAAGGCTTGGCCGGTGCCGGGGTCGGCGCCGCCCCCTTGGCCGCGTCGGGCTTGCCCGCAGGGCGCGCGGTGAACGAGTCGTTGGCAAAGGGCGGGGTGGGGCGCGCCGGGGCGCTGGCCAGGAGGCGGtggg                               >  2:319979/1‑134 (MQ=255)
                                                                                                aCCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCCTGGCCGCGTCGGGCTTGCCCGCCGTCAGCGCGGTCAACGCGTCGTTGGGAAAGGGCGGGTTGGGGGGCGCCGGGGCGCTGGGCCTCAGGCGGGCGAg                              >  2:322148/1‑137 (MQ=255)
                                                                                                  cAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCt                            <  1:322148/137‑1 (MQ=255)
                                                                                                            gccggtgccggtGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCtt                  <  7:379218/137‑1 (MQ=255)
                                                                                                                  gccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCGAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAg            <  6:281313/137‑1 (MQ=255)
                                                                                                                  gccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAg            <  3:76330/137‑1 (MQ=255)
                                                                                                                  gccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAg            <  7:293694/137‑1 (MQ=255)
                                                                                                                  gccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAg            <  6:159258/137‑1 (MQ=255)
                                                                                                                            gcgcCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGctttcttt  <  2:195370/137‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                
AACGCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTT  >  KT2440/6063863‑6064123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑