Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 3,061,793 G→A S592N (AGC→AAC)  qedA_2 → Quinoprotein alcohol dehydrogenase (cytochromec)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,061,7930GA90.5% 120.9 / ‑5.6 42S592N (AGC→AAC) qedA_2Quinoprotein alcohol dehydrogenase (cytochromec)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (28/10);  minor base C (1/3);  total (29/13)
Fisher's exact test for biased strand distribution p-value = 8.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGT  >  KT2440/3061659‑3061924
                                                                                                                                      |                                                                                                                                   
ggTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                   <  7:352919/136‑1 (MQ=255)
cGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                   <  3:382339/137‑1 (MQ=255)
      ggTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGc                                                                                                                             >  2:120423/1‑137 (MQ=255)
           tctcCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa                                                                                                                        <  2:55521/137‑1 (MQ=255)
              ccGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCAAGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACgc                                                                                                                     >  8:258272/1‑137 (MQ=255)
                ggCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACgcct                                                                                                                   >  3:166221/1‑137 (MQ=255)
                   tGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACgcctgcc                                                                                                                >  2:213269/1‑137 (MQ=255)
                          gcgcGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGcc                                                                                                         >  4:58118/1‑137 (MQ=255)
                                  ccGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTtgctg                                                                                                 <  3:58118/137‑1 (MQ=255)
                                  ccGCTGGGGGGCGGCGAAGTTGCCAAACGGGTCAAGGACTTCAACCAGGGCTGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTtgctg                                                                                                 <  5:56759/137‑1 (MQ=255)
                                       gtgGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCt                                                                                            <  1:175665/137‑1 (MQ=255)
                                           ggcggcGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATc                                                                                        >  5:263505/1‑137 (MQ=255)
                                                cgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTgg                                                                                   >  8:150408/1‑137 (MQ=255)
                                                           aaggggTAATGGCCTTCAACCACGGGGGCTTGCTCTGTACCTTCATGTTCCCCAACCAGTTCCCGCAAACGCAAACGGACACGCCAAAACGCCTGCCTACTGCCGCTTGCTGCCGGTGAGCGGGGCAAAGCGGCCct                                                                        <  4:316860/134‑1 (MQ=255)
                                                                         ttCAACCAGGGCGGAATGCTCTGGACCTTCAAGTTGCCCAAGCATTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGACAAGCGGCCCTACTACCAAATGACg                                                          <  4:137945/137‑1 (MQ=255)
                                                                             aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACgagac                                                      >  7:266316/1‑136 (MQ=255)
                                                                             aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGagag                                                      >  5:104320/1‑137 (MQ=255)
                                                                             aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGagag                                                      >  4:74774/1‑137 (MQ=255)
                                                                             aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGagag                                                      >  3:173884/1‑137 (MQ=255)
                                                                             aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGagag                                                      >  2:235221/1‑137 (MQ=255)
                                                                                    cggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGcg                                               >  1:292219/1‑136 (MQ=255)
                                                                                    cggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCt                                               >  2:5506/1‑137 (MQ=255)
                                                                                    cggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCt                                               >  5:236521/1‑137 (MQ=255)
                                                                                    cggcATGCTCTGCACCTTAAAGTTGCCCAACCAGTTGCAGCAAACGGCAACCGTCAAGCCATAAGGCCTGCCTACTGCCGCTTGCTGCCGGTGATCGTTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCt                                               <  6:201547/137‑1 (MQ=255)
                                                                                     ggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTg                                              >  7:237315/1‑137 (MQ=255)
                                                                                      gcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCAGATCGGTGGCAAGCGGCCCTACTACCAAATGACCAGAGCCCCGCTGc                                             >  5:393853/1‑137 (MQ=255)
                                                                                         tGCTCTGGACCTTAAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAAAGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGATGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCAAGCTGCCaa                                          >  7:201777/1‑137 (MQ=255)
                                                                                              tggaaattCAATTTGCCAATCCAGGTGCAGCAAACGGAAAACGTCAACCCAAAACCCCTCCATCGTGCCGTTTGCTGCGTCTGTCCGGTGCCAAGGGGCCCTACTACCAAATGACGATAGCCCCGCTGCCAACGATg                                     <  2:42272/131‑1 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCATCAAACGGCAACCGTCAAGCCATAACGCCTGCCTACTGCCGCTTTCTGCCGCTGATCGGTTGCAAGCGGCCCTACTACCAAATGACGAGAGCCCAGCTGACAACGATGCATTCGACCg                           >  7:249508/1‑137 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATCACGCCTGCCTACTGCCGCTTGCTGCCCCTGATCGGTGGCAAGCGGCCCGACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGcccc                           >  4:201574/1‑136 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCg                           >  6:314104/1‑137 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCg                           >  2:45854/1‑137 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGAAGCATTCGcccc                           >  8:223958/1‑136 (MQ=255)
                                                                                                         gTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCAgg                          >  8:244905/1‑137 (MQ=255)
                                                                                                            ccccATGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAAGCCCTGCCTACTGCCGCTTGCGGCCGCTGATCGGTGGCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAc                       <  3:157412/136‑1 (MQ=255)
                                                                                                            ccccACGCCGTGGCACAAACCGGCAACCGCCACGCCAAACCGCCTCCCTACTCCCGCTGGCGGCCGCTTAGCGGGGCCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAc                       <  7:31280/136‑1 (MQ=255)
                                                                                                                aaGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCgg                   >  5:197060/1‑137 (MQ=255)
                                                                                                                    tgTTCCAGCAAACGCCAAACGTCACGCCATACCGCCTGCCTACTGCCGCTTGCTGCCGCTGAGGGGGGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGct               <  2:46336/136‑1 (MQ=255)
                                                                                                                    aGTTGCCTCAAACGGCAAACTTCAAGCCATACCGCCTGCATCCTCCCGCTTGCTGCCGCTGATCGGTGGCATGGGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACCCGGGGct               <  8:103086/137‑1 (MQ=255)
                                                                                                                          agcaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAc         <  6:104320/137‑1 (MQ=255)
                                                                                                                          agcaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAc         >  7:207507/1‑137 (MQ=255)
                                                                                                                          agcaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAc         >  1:8888/1‑137 (MQ=255)
                                                                                                                          agcaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAc         >  3:55846/1‑137 (MQ=255)
                                                                                                                                 ggCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGt  >  1:46336/1‑137 (MQ=255)
                                                                                                                                      |                                                                                                                                   
CGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGT  >  KT2440/3061659‑3061924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑