| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,757,234 | Δ1 bp | 100% | coding (776/834 nt) | CJ019_05400 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,757,234 | 0 | A | . | 90.9% | 127.2 / 2.9 | 33 | coding (776/834 nt) | CJ019_05400 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); major base . (28/2); minor base T (0/2); total (28/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTT > KT2440/5757107‑5757351 | cAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCCCGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGaaaaacgc > 6:5204/1‑132 (MQ=255) gAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGCCCCGTGAAGCTGAAGAGGCTCGCAAGAAGCCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCcagca > 1:42157/1‑134 (MQ=255) aCAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGCAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCCCGCACGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCCCcagcag > 5:274665/1‑133 (MQ=255) cGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgc > 4:162338/1‑135 (MQ=255) cGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgc > 2:269384/1‑135 (MQ=255) tGGCCAAGGTCTTGAAGACCATCGAGGAACCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCCCGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGACGCGCTGGccgccgccg > 5:4462/1‑135 (MQ=255) tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg > 4:3026/1‑135 (MQ=255) tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg > 2:15698/1‑135 (MQ=255) tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg > 2:274340/1‑135 (MQ=255) tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg > 3:225703/1‑135 (MQ=255) cTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCAGCCcgcgcc > 7:263277/1‑134 (MQ=255) aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGc > 7:52412/1‑136 (MQ=255) cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGGGa > 4:158448/1‑135 (MQ=255) cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGa > 1:216553/1‑135 (MQ=255) cGAGGAAACCCTCGCCCGCCAGCCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa > 3:183192/1‑135 (MQ=255) cggggAAGCTTAAGAGGCACGAAAGAAGGCCCGGCGGGCGCAGCTGTAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg < 7:48343/132‑1 (MQ=255) ggTGACGCTGATGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg < 4:139896/134‑1 (MQ=255) gTGACGCTGAAGCGGCACGCAAGACGGCCCTGCTGGCGCAGCCGGACGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGa < 2:30400/135‑1 (MQ=255) aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAACACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGCGCCAGTGGACCCGCCGaaaaa > 2:59042/1‑135 (MQ=255) aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa > 1:72510/1‑135 (MQ=255) aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa > 1:55512/1‑135 (MQ=255) aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAg > 8:197103/1‑136 (MQ=255) aaGAGGCACGCAAGAAGGCCCTGCTGGCGCATCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGACTGCAGAGCCAGTGGAACCGCCGAAAAAGGccc > 2:199936/1‑135 (MQ=255) acccacgaagGCCGTGCGGGCGCAGCAGGACGC‑GTAAAAACGCCGCCAGCAGGACGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTaccac < 5:238243/129‑1 (MQ=255) cGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCAccc > 7:263557/1‑136 (MQ=255) cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAACGGCCCGTACCACCCt > 1:45021/1‑135 (MQ=255) cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCAcccg > 2:105989/1‑134 (MQ=255) cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTc > 8:139342/1‑136 (MQ=255) gaagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCAGCGCCAGTGGAACCGCCGAAAAAGGGCCGTACCCCCCTCggg > 7:239477/1‑136 (MQ=255) gaagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCgg > 3:182383/1‑135 (MQ=255) gagGCCCTGGTGGGGCACCAGTAAGC‑GAAAAAACGCCGCCAGCTGGAAGCGCGGGCGGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGc < 8:263277/135‑1 (MQ=255) gctgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGt > 1:159929/1‑135 (MQ=255) gctgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGGGCGCCTGCCGCGCCAGGGGCACCGCCGAAAAAGGCCCGTTCCACCCACGGGCCGATggg > 2:141165/1‑134 (MQ=255) tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt > 1:68830/1‑135 (MQ=255) | CAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTT > KT2440/5757107‑5757351 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |