| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,670,685 | A→C | 27.7% | intergenic (+22/‑109) | thrC_2 → / → CJ019_01569 | Threonine synthase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,670,685 | 0 | A | C | 27.7% | 36.1 / 2.7 | 40 | intergenic (+22/‑109) | thrC_2/CJ019_01569 | Threonine synthase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (23/4); new base C (5/6); total (28/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCCCTATGTGT > KT2440/1670554‑1670812 | aCCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTgcagcg > 2:171794/1‑135 (MQ=255) gCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGccc > 3:274141/1‑135 (MQ=255) ccTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGGAGCGCAGCCCCAAc > 4:15361/1‑135 (MQ=255) tCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGtc > 3:96702/1‑133 (MQ=255) agCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCCCGCCCCCCCAACCTCTCACCCatttaacg > 6:220771/1‑131 (MQ=255) tGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGt > 3:178450/1‑135 (MQ=255) ggCACGGGGCTGGAAAACTAGCTCAAGGCTGTACAGGGATTTGACAGCCTGCATGCAAA‑CCGCGCCAAGCCGTTGTATTTGCTTGTCTTTTTGGGCGGCCTCGCTGCCCCAACGTCTCAGCCTTTTCCCTCCTGTc < 8:79998/135‑1 (MQ=255) gtcggggCAAACCACCTGATGCTTGTACAGGGTTTTGTCACCCTGC‑CGGCAACCCGCGTAACGCGTCTTTATTTGGATGTGTTTTGGGGGGGCTGGGCACCCAAAACGTCTCAGCCTTTTCCCTCCTGTCTATCa < 2:223529/130‑1 (MQ=255) tGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCCGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGaa > 2:185674/1‑134 (MQ=255) tGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGaa > 4:35841/1‑134 (MQ=255) ccAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCAGCCGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTGTATCAGAAACAt > 3:271703/1‑135 (MQ=255) caCGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCCCAGCCCCCACGTCTCAGCGTTTTTCCTCCTGTGTATAAGAAACAtac > 7:232739/2‑133 (MQ=255) aaCGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCACCGCCTTTTACCTCCTGTCTATAAGAAACATc > 3:68269/1‑134 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCAGCCCCCACGGCGCAGCCGTTTGCCTCCGGGCTATGAGAAACCTCCTAtt > 7:114372/1‑134 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCCACGTCTCCGCCTTTTCCCGCCTGTCTATCAGAAACAGCCTATTCa > 7:21394/1‑136 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCCGCCTTTTCCCTCCTGTCTATCAGAAACATCCTAtt > 8:40690/1‑134 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTAtt > 8:207967/1‑134 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTAt > 4:33005/1‑133 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTc > 5:72384/1‑135 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATAAGAAACATCCTATTc > 5:49719/1‑135 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCGGCCGCGCCGCCCCCCCCTGTCAGCCCTTTCCCGCCGGGCTATCAACAACAACCGATTc > 5:274474/1‑135 (MQ=255) tGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTAAGc > 7:30057/1‑136 (MQ=255) aGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCACCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGcc > 7:167056/1‑136 (MQ=255) tttGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCGGCCGCCCCCCCCCCCCGGGGCAGCCGTTTACCTCCCGTCGATAACAAACAAACGATTAAGCCGGCCCGGCGcccaa > 1:272711/1‑131 (MQ=255) tGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTGCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCAt > 7:167369/1‑135 (MQ=255) tGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCAt > 8:98529/1‑135 (MQ=255) cagccagcACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCCGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCGATCAGAAACAACCTATTCAGCCTGCCGGGCCCCAAGCATaaa > 6:246717/1‑135 (MQ=255) cagccagcACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCATTTCCCGCCTGTGTATGAGAAACATCCAATTCCCCCAGCCGGGCGCCAAGCATaaaa > 7:252113/1‑135 (MQ=255) cagccagcACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCAGCAGCGCAGCCCCAACGTCTCAGCCATTACCCTCATGTCTATCAGAAACATCCTAATCACCCAGCCGGGCGCCAAGCATaa > 4:25128/1‑134 (MQ=255) cGGCAC‑CGGCGGCAAGCCGTTGTAATGGCAGGGGGTTGGGGGGGGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGt < 4:121929/135‑1 (MQ=255) cGGCAC‑CCGGGCCACGCCGCTGTAATGCCAGGGGGTGGGGGGCGGCGGCGCCGCCCCCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGt < 1:171794/135‑1 (MQ=255) ggCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCGCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGtc > 6:47351/1‑135 (MQ=255) ggaacgCCGCTTTAATTGCATGGGGTTGGGGGCGCCAGCGCCCCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCa < 6:49719/130‑1 (MQ=255) ggCTGTAATTGCTTGGGGTGTGGGGCGGCCGCGCCGCCCCCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGc < 8:252113/135‑1 (MQ=255) aaaTGGCAGGGCGTGTGGGGCTCCCGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCAt < 7:93576/134‑1 (MQ=255) aaTGGCAGGGCGTTGGGGGCGGCCGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCAt < 2:11353/134‑1 (MQ=255) ttGCATGGCTTTGGGGGTTCCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCa < 3:207217/135‑1 (MQ=255) ttGCAGGGGGTTGGGGGCGGCCGCGCCCCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCa < 4:271703/135‑1 (MQ=255) cGTTTGGGGCTGCAGCGCCGCCCCAACGTCTCAGCCTTTTCCCTCCGGTCTTTCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGcccg > 3:118012/1‑134 (MQ=255) tttGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCCCTAt < 4:178450/135‑1 (MQ=255) gggCTGCCGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCCCTATgtgt < 3:33005/135‑1 (MQ=255) | ACCTCAGCGACCTGTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAA‑CCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCCCTATGTGT > KT2440/1670554‑1670812 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |