Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,465,846 G→A 8.1% A436V (GCG→GTG)  CJ019_02325 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,465,8460GA 8.1% 150.6 / 2.7 49A436V (GCG→GTG) CJ019_02325hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/18);  new base A (2/2);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACCTGCGCTGCACCGCGGTGGGCCATCCAGTTCTCGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTGCGCCAGCGGCGGGTGCGGGTGTCCAATACCAGCAG  >  KT2440/2465712‑2465970
                                                                                                                                      |                                                                                                                            
tGACCTGCGCTGCACCGCGGTGGGCCATCCAGTTCTCGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGAccg                                                                                                                              <  5:146950/135‑1 (MQ=255)
    cTGCGCTGCCCCGCGGTGGGCCATCCAGTTCTCGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACCCGATGAccgcc                                                                                                                            >  5:162750/1‑133 (MQ=255)
    cTGCGCTGCACCGCGGTGGGCCATCCAGTTCTCGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGAccgccg                                                                                                                           >  8:162195/1‑134 (MQ=255)
     tGCGCTGCACCGCGGTGGGCCATCCAGTTCTCGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGaa                                                                                                                         >  4:107442/1‑135 (MQ=255)
         cTGCACCGCGGTGGGCCATCCAGTTCTCGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGga                                                                                                                      >  4:28518/1‑134 (MQ=255)
               cgcgGTGGGCCATCCAGTTCTCGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGGCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGa                                                                                                               <  5:228765/135‑1 (MQ=255)
                    tGGGCCATCCAGTTCTCGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGt                                                                                                          >  5:222919/1‑135 (MQ=255)
                     gggCCATCCAGTTCTCGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGtg                                                                                                         <  3:171663/135‑1 (MQ=255)
                      ggCCATCCAGTTCTCGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGtgc                                                                                                        <  7:17513/135‑1 (MQ=255)
                        ccATCCAGTTCTCGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGtgctt                                                                                                      <  4:97846/135‑1 (MQ=255)
                        ccATCCAGTGCTCGGCATCCCCCCGCAGCGGGTCGCCCTGCCAGCTGAACACCTTCTGACCTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGtgctt                                                                                                      <  2:274040/135‑1 (MQ=255)
                                   tcGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCa                                                                                           >  1:191432/1‑135 (MQ=255)
                                     ggCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCACCGAAGGATGATc                                                                                                                 >  7:112744/1‑111 (MQ=255)
                                     ggCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCACCGAAGGATGATc                                                                                                                 >  7:115899/1‑111 (MQ=255)
                                     ggCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCACCGAAGGATGATc                                                                                                                 <  8:112744/111‑1 (MQ=255)
                                     ggCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCACCGAAGGATGATc                                                                                                                 <  8:115899/111‑1 (MQ=255)
                                      gCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTAACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAAATCACTCa                                                                                       >  7:2534/1‑136 (MQ=255)
                                       cATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCa                                                                                       <  6:187701/135‑1 (MQ=255)
                                        aTCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAgc                                                                                     >  7:265796/1‑136 (MQ=255)
                                        aTCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACAGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAg                                                                                      >  3:59962/1‑135 (MQ=255)
                                            accaGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCt                                                                                  <  6:54176/135‑1 (MQ=255)
                                            accaGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCt                                                                                  <  3:84664/135‑1 (MQ=255)
                                                  agcGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGt                                                                           <  8:265796/136‑1 (MQ=255)
                                                         aGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGt                                                                                                          >  1:164448/1‑98 (MQ=255)
                                                         aGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGt                                                                                                          <  2:164448/98‑1 (MQ=255)
                                                         aGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCcagca                                                                     >  2:120416/1‑135 (MQ=255)
                                                         aGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGAGTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCcagca                                                                     >  6:115768/1‑135 (MQ=255)
                                                            ccagccagCTGTACACCTGATGCACTGTCTCGATTAGCTTCACCCCGAAACTCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGc                                                                  <  6:162750/135‑1 (MQ=255)
                                                                     tGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCt                                                         <  2:191432/135‑1 (MQ=255)
                                                                                 gCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGc                                             >  6:138737/1‑135 (MQ=255)
                                                                                 gCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCt                                            >  7:13798/1‑136 (MQ=255)
                                                                                         tcGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTg                                     >  3:12742/1‑135 (MQ=255)
                                                                                          cGATTAGCTTCACCCCGAAAATCGGCTCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTgc                                    >  2:133223/1‑135 (MQ=255)
                                                                                          cGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGCCGGCTTGGCCAGGTTGCTTTCGCTgc                                    >  3:232171/1‑135 (MQ=255)
                                                                                          cGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGcc                                                    >  4:192274/1‑119 (MQ=255)
                                                                                          cGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGcc                                                    <  3:192274/119‑1 (MQ=255)
                                                                                          cGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGAGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTgc                                    >  6:271825/1‑135 (MQ=255)
                                                                                               atcTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAATGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCCCTTCCCAGTCAAGCAGGCCGGACCTCTTGGCCAGGTTGCTTTCGCTTCGcccg                               >  2:99134/3‑133 (MQ=255)
                                                                                                    cACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGgat                                                                                                                     >  7:36510/1‑44 (MQ=255)
                                                                                                    cACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGgat                                                                                                                     <  8:36510/44‑1 (MQ=255)
                                                                                                    cACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTGCGCCAgcggcg                          >  5:65600/1‑135 (MQ=255)
                                                                                                    cACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTGCGCCAgcggcg                          >  2:209288/1‑135 (MQ=255)
                                                                                                    cACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTGCGCCAgcggcg                          >  1:171259/1‑135 (MQ=255)
                                                                                                    cACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTGCGCCAgcggcg                          >  1:163177/1‑135 (MQ=255)
                                                                                                    cACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTGCGCCAGcggcgg                         >  8:111677/1‑136 (MQ=255)
                                                                                                      ccccGAAATTCGGCGCAGGTGAAACGATGTCCGCCTAAGGATGATAGAGCAGTGCTTGCTGCAATTCACTCAGCTCTTCCCATTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTGCGCCAGCGgcggg                        <  5:41360/135‑1 (MQ=255)
                                                                                                       cccGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGGCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTGCGCCAGCGgcgggtg                      <  8:2534/136‑1 (MQ=255)
                                                                                                        ccGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTGCGCCAGCGgcgggtgc                     >  7:50650/1‑136 (MQ=255)
                                                                                                        ccGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTGCGCCAGCGgcgggtg                      >  6:33767/1‑135 (MQ=255)
                                                                                                                            cacGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTGCGCCAGCGGCGGGTGCGGGTGTCCAATACcagcag  <  3:107442/135‑1 (MQ=255)
                                                                                                                                aTGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCtt                                                        <  2:183415/77‑1 (MQ=255)
                                                                                                                                aTGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCtt                                                        >  1:183415/1‑77 (MQ=255)
                                                                                                                                      |                                                                                                                            
TGACCTGCGCTGCACCGCGGTGGGCCATCCAGTTCTCGGCATCCACCAGCAGCGGGTAGCCCAGCCAGCTGAACACCTTCTGCACTGTCTCGATTAGCTTCACCCCGAAAATCGGCGCAGGTGACACGATGACCGCCGAAGGATGATCGAGCAGTGCTTGCTGCAATTCACTCAGCGCTTCCCAGTCCAGCAGGCCGGACGGCTTGGCCAGGTTGCTTTCGCTGCGCCAGCGGCGGGTGCGGGTGTCCAATACCAGCAG  >  KT2440/2465712‑2465970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑