Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,092,717 C→T 8.1% A316T (GCG→ACG)  CJ019_03854 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,092,7170CT 8.1% 136.6 / 2.2 49A316T (GCG→ACG) CJ019_03854hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (28/17);  new base T (2/2);  total (30/19)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAGCACCAATGTTGCAGCCGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTACCGACATCCACACCCAGCCCGCT  >  KT2440/4092587‑4092849
                                                                                                                                   |                                                                                                                                    
tAAGCACCAATGTTGCAGCCGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAg                                                                                                                                   >  2:75401/1‑134 (MQ=255)
aaacccccAATGGTGCAGCCGAAAGCCAGCCGCGACCCATCGCCCAGCAGACGCCCGCCAATCCCGGCGGCCA‑CCAGCCCCGGCAGCGGAATCTTCCGGTTGGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGc                                                                                                                                  <  4:172179/129‑1 (MQ=255)
     aCCAATGTTGCAGCCGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCCGCAAGGc                                                                                                                             >  5:21081/1‑135 (MQ=255)
     aCCAATGTTGCAGCCGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGc                                                                                                                             >  1:186834/1‑135 (MQ=255)
     aCCAATGTTGCAGCCGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGc                                                                                                                             >  8:11426/1‑135 (MQ=255)
     aCCAATGTTGCAGCCGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGc                                                                                                                             >  3:61016/1‑135 (MQ=255)
     aCCAATGTTGCAGCCGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAAACACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGc                                                                                                                             >  1:222786/1‑135 (MQ=255)
     aCCAATGTTGCAGCCGTAAGCAAGGCGCGAACCATAGCCAAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAACCGACCGGCCAGGCCCGCCGCCAGCAACGc                                                                                                                             >  1:241115/1‑135 (MQ=255)
          tGTTGCAGCCGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCC‑CCAGCGCCGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCa                                                                                                                        >  3:156281/1‑135 (MQ=255)
          tGTTGCAGCCGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCa                                                                                                                        >  4:15432/1‑135 (MQ=255)
           gtggccgCCGTAAGCCCGGCGGCAACCTTGGCCCAGCTGATGGCCGCCATTCCGGGGGGCCA‑CCGGCGACGGCTGGGGATTCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCa                                                                                                                        <  6:145327/128‑1 (MQ=255)
             tGCAGCCGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTAc                                                                                                                    >  8:52371/1‑136 (MQ=255)
                 gCCGTACCCCCGGCGCTAACAATCGCCCCGCTGCCGGCCGCAAATCAGGCCGGCCC‑CCCGCGCCGGCAGCGGAAGATTCTGTTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATg                                                                                                                <  8:229505/136‑1 (MQ=255)
                 gCCGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATg                                                                                                                >  7:242627/1‑136 (MQ=255)
                  ccGTACGCCAGGCGCGAACAATAGCCCCGCATACGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCACAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATg                                                                                                                <  1:188619/135‑1 (MQ=255)
                  ccGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATg                                                                                                                >  4:190021/1‑135 (MQ=255)
                    taacgCCCGGCGCTACCCATAGCCCCGCATAAGGCCGCAAATCCCTGCGCCCA‑CCAGCGAGGGCCGCGGAATGTCCTGTTTTGGCCAAACGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGcc                                                                                                              <  6:142823/131‑1 (MQ=255)
                    gTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGTCAGCAAGGCACCCAGTACGATGcc                                                                                                              >  5:9747/1‑135 (MQ=255)
                    gTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGTCAGCAAGGCACCCAGTACGATGc                                                                                                               >  5:6197/1‑134 (MQ=255)
                    gTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGcc                                                                                                              >  8:131566/1‑135 (MQ=255)
                               gcgAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTAc                                                                                                  >  7:229505/1‑136 (MQ=255)
                               gcgAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGACCATTa                                                                                                   >  3:247042/1‑135 (MQ=255)
                                          cccACCACACGGCCGCAAATCAC‑GCCGCCACCCAGGTACGGCAGCGGAAGCTTCAGTTTTGGCGAAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATg                                                                                        <  6:114582/135‑1 (MQ=255)
                                                  aaGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGcc                                                                                 >  3:155166/1‑134 (MQ=255)
                                                  aaGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGcc                                                                                 >  3:15206/1‑134 (MQ=255)
                                                  aaGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATCTCCTGcc                                                                                 >  1:229113/1‑134 (MQ=255)
                                                       cgccAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCACGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAgcg                                                                           >  1:146624/1‑135 (MQ=255)
                                                       cgccAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAgcg                                                                           >  1:77734/1‑135 (MQ=255)
                                                          cAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAgcggcg                                                                        >  3:201727/1‑135 (MQ=255)
                                                            aTCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCa                                                                      >  6:213709/1‑135 (MQ=255)
                                                            aTCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCa                                                                      >  2:38109/1‑135 (MQ=255)
                                                                 ggccgCCA‑CCCGCGCCGGCAGCGGAAGCTTCTGGTGGGGCGCAACGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCaaa                                                                <  7:227520/132‑1 (MQ=255)
                                                                   gggCCA‑CCGGCGCCGGCAGGCCATTCTTCTGGTTTGGCGCCCCGCCCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAg                                                               <  1:213846/134‑1 (MQ=255)
                                                                              ggACGGAAGCGTAATCTTCAGGTTTGGCGCAACGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCAtt                                                     <  2:77734/134‑1 (MQ=255)
                                                                              ccaCGCCAGCGTAATCTGCTGGTTGGGCGCAACGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCAtt                                                     <  4:61016/133‑1 (MQ=255)
                                                                              cGACGCCAGCGATAGCTGCAGGGGGGGCTCAACGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCGCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGCCaat                                                     <  4:229849/135‑3 (MQ=255)
                                                                                             ctcccggTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCa                                      <  8:66214/130‑1 (MQ=255)
                                                                                                    ttgggCCCAACGCCCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACAcc                               <  1:75401/132‑1 (MQ=255)
                                                                                                         cgcAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCt                          >  3:245244/1‑135 (MQ=255)
                                                                                                           cAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTAcc                       >  8:110520/1‑136 (MQ=255)
                                                                                                           cAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTAc                        >  3:203421/1‑135 (MQ=255)
                                                                                                               gcgcCCGGCCAGGCCCGCCGTCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTACCGAc                    <  6:6197/135‑1 (MQ=255)
                                                                                                               gcgcCCGGCCAGGCCCGCCGTCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTACCGAc                    <  6:9747/135‑1 (MQ=255)
                                                                                                               gcgcCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTACCGAc                    <  2:222786/135‑1 (MQ=255)
                                                                                                                 gcCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTACCGACAt                  >  2:64654/1‑135 (MQ=255)
                                                                                                                  cccGGCCAGGCCCGCCGCCAGCAAGGCACCCAGGACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTACCGACATc                 <  2:239649/135‑1 (MQ=255)
                                                                                                                       ccAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTACCGACATCCacac            <  3:256205/135‑1 (MQ=255)
                                                                                                                          ggCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGACTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTACCGACATCCACACCCa         <  6:21081/135‑1 (MQ=255)
                                                                                                                             ccgccgCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTACCGACATCCACACCCAGccc     >  7:149693/1‑136 (MQ=255)
                                                                                                                              cgccgcCAGCAAGGCACCCAGTACGATGCCCAGGt                                                                                                         <  4:110686/35‑1 (MQ=255)
                                                                                                                              cgccgcCAGCAAGGCACCCAGTACGATGCCCAGGt                                                                                                         >  3:110686/1‑35 (MQ=255)
                                                                                                                               gccgccAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTACCGACATCCACACCCAGCCCg    <  2:182813/135‑1 (MQ=255)
                                                                                                                                 cgccAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTACCGACATCCACACCCAGCCCGCt  <  4:155166/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                    
TAAGCACCAATGTTGCAGCCGTAAGCCAGGCGCGAACCATAGCCCAGCAGAAGGCCGCCAATCACTGCGGCCA‑CCAGCGACGGCAGCGGAATCTTCAGGTTTGGCGCAAAGCGCCCGGCCAGGCCCGCCGCCAGCAAGGCACCCAGTACGATGCCCAGGTCCATTACCGTGGTGATGTCCTGCCACAGCGGCGCAGCCAAAGCCTTGGCATTGGCCGGTGCCTGCCAGAACACCCAGCTACCGACATCCACACCCAGCCCGCT  >  KT2440/4092587‑4092849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑