| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,289,845 | T→G | 41.4% | intergenic (+119/‑106) | CJ019_04021 → / → gloA | hypothetical protein/Lactoylglutathione lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,289,845 | 0 | T | G | 41.4% | 7.1 / 2.4 | 28 | intergenic (+119/‑106) | CJ019_04021/gloA | hypothetical protein/Lactoylglutathione lyase |
| Reads supporting (aligned to +/- strand): ref base T (5/8); new base G (7/2); total (18/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.91e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑TGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCT > KT2440/4289717‑4289976 | ggTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCAAATTTCAGCGCCC‑CGCCgtt > 3:164581/1‑135 (MQ=255) aCGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGGGCCC‑AGccggtggg > 1:152496/1‑129 (MQ=255) aCGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑GGCCGTTGCAg > 7:45546/1‑136 (MQ=255) aCGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTCGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTAAGGGCCC‑GGACttttga > 5:204871/1‑132 (MQ=255) gatgaAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCCCATGCGGGCCTCTAATTTCAGGGCCCAGGCATTTGCAggcg > 1:53135/1‑133 (MQ=255) aaTGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑TTCCTTTTCAGGgcgggtt > 4:159372/1‑130 (MQ=255) aaTGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑TGCCGTTGCAGGGCGCgttgc > 7:217662/1‑134 (MQ=255) acaTAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑AGCCTTTGCAGGGCGCGTTGATTTtcg > 8:269167/1‑135 (MQ=255) attCACACTTTTTACACAAGCACTGGCTAATTATCCAACCTGCTAAAGGACTCGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑TGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGgc < 7:69747/135‑1 (MQ=255) acTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTATTTTCAGCGCCC‑CGCCGTTTCAGGGGGCGTTCAATTTGGCAATAGgcgcccg > 5:40087/1‑132 (MQ=255) ttCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑TGCCTTTGCAGGGCGCGTTCATTTGCGCAATCGGCGCACTGCCGTa > 4:39174/1‑135 (MQ=255) cacaAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑GGCCATTGCACGGCGCGTTCCTTTGCGCCATCGGCGCCCAGCCGGAt > 8:131856/1‑134 (MQ=255) gTATCGAAACAGCTAAAGGACTAGCGACCCCATCCCGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑TGCCGTTGCAGGGCGCGTTAATTTTCGGAATAGGCGCACTGCCGTATCATGGCCAGCctggctg > 6:254721/1‑135 (MQ=255) cGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑GGGCGTTGCCTGGGGCGGGTATTTGAGCGAACGGCGCCCGGCCCGATACTGGGCAGCCGGTCTGgttt > 6:271020/1‑135 (MQ=255) gAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑TGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTg < 6:270146/135‑1 (MQ=255) aaGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑AGCCTTTGCAGGGCGGGTTGATTTGGGCAATCGGCGCACTGCCGTATCATGGCCCGCCGGTCTGTTTTTCCGGCGCCCt > 4:254256/1‑135 (MQ=255) gCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑TGCCTTTGCAGGGCGCCGTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGc > 7:138995/1‑136 (MQ=255) accccgcgCCCCCACTGGCGGGCCGCTAATTTCAGCGCCC‑TCCGTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGtct < 3:39174/131‑1 (MQ=255) aTCCCGCGCCCGCCCTTGCGGGCCTCTATTTTCAGCGCCC‑TGCGTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGtct < 6:40087/135‑1 (MQ=255) aCGCGCCCCCAAATGGGGGCGGCTAATTTCAGCGCCC‑TGCCTTTGCCGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAgg < 7:269167/136‑1 (MQ=255) ccGCAACTGGGGGCCTTTAATTTCCGCCCCG‑TGCGTTTGCTGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTc < 6:59366/135‑1 (MQ=255) cGCACATGCGGGCCTCTAATTTCAGCGCCC‑GGCCGTTGGCGGGCGCGTTAATTTGCGCAATCGGCGCACTGCCGGATCAAGGCCCGCCGGTCTGTTTTTCCGGCGCCCGGGGCCCCGCCCCGGCTCAGGAGTTacc > 8:122065/1‑136 (MQ=255) gCACATGCGGGCCTCTAATTTCAGCGCCC‑GGCCGTTGCAGGGGGCGTTGATTTTCGCAATAGGCGCCCTGCCGGTTCATGGCCAGCCGGGGTGTTTTTGCCGCGCCCGGCGCCCCGCCCCGGCTCAGGAGTTgcc > 8:45799/1‑135 (MQ=255) acaTGCGGGCCTCTAATTTCAGCGCCC‑CGCCATTTGAGGGCGCGTTGATTTTGGCCATCGGCGCACGGGCGTATCATGGCCCGCCTGTCGGTTTTGGCGGCGCCCGGCGCCCGGCCCCGGCGCCGGAGTTcccct > 2:8647/1‑133 (MQ=255) gggCCTCAAATTTCAGCCCCC‑GGCGTTGCCGGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTg < 8:10848/136‑1 (MQ=255) ccTCTAATTTCAGCGCCC‑TGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCa < 4:112687/135‑1 (MQ=255) aGCGCCG‑TGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCaa < 2:38253/134‑1 (MQ=255) gcCC‑GGCCTTGCCTGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACcct < 7:45799/136‑1 (MQ=255) | GGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCC‑TGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCT > KT2440/4289717‑4289976 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |