| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 44,301 | (T)9→8 | intergenic (+132/‑359) | oprD_1 → / → czcR_2 | Porin D/Transcriptional activator protein CzcR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 44,293 | 0 | T | . | 87.9% | 105.6 / 13.2 | 35 | intergenic (+124/‑367) | oprD_1/czcR_2 | Porin D/Transcriptional activator protein CzcR |
| Reads supporting (aligned to +/- strand): ref base T (1/1); major base . (13/16); minor base G (4/0); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGT > KT2440/44169‑44401 | aTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCGTTTTTTTTg > 6:157757/1‑134 (MQ=255) cTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAAtt > 6:197410/1‑133 (MQ=255) cTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCa > 8:129193/1‑135 (MQ=255) cTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCa > 6:83274/1‑135 (MQ=255) ccAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAg < 5:272900/135‑1 (MQ=255) tATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGtt < 5:256943/135‑1 (MQ=255) aTTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGAtt < 5:32342/135‑1 (MQ=255) tGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCCCCTTGAGTGGCTTAAGTGCTCCTTTAGTATAAGGTGGATAATTAGCGCCCATTGGGCGCC‑TTTTTTTTGCCTATAAATCAAGTAGGAGTTCGATTg > 4:233672/1‑134 (MQ=255) tGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGt < 8:222626/135‑1 (MQ=255) cGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTTGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATACAAGTAGGCGTTCGATTGTAc > 4:121166/1‑134 (MQ=255) cGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTG‑CTAAAATTAAATTATGCGTTGGATTTTAcg > 2:142524/1‑135 (MQ=255) ggTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGTTGGATATTTGGCGCCCATTGGGCG‑CGTTTTTTTTGCCTAAAATTAAAGTAGGCGTTCGATTGTAcgca > 6:153238/1‑135 (MQ=255) tGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcatcgc < 2:14716/134‑1 (MQ=255) tGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcatcgc < 7:129193/134‑1 (MQ=255) gTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAAGCATCGCATGACg > 6:198877/1‑135 (MQ=255) tAAAACGAGCATTACGCATTCAGCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGTCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg < 3:254296/134‑1 (MQ=255) tAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg < 1:142524/134‑1 (MQ=255) aCGATCATTAGGCATTCACCTTGAGTGGCTTAAGTTCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGaa < 3:138439/135‑1 (MQ=255) aTTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCAt < 3:134720/135‑1 (MQ=255) tACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCCTGCCGGGGAAGTTCATGa > 4:177112/1‑135 (MQ=255) tACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa < 5:83274/135‑1 (MQ=255) gCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACt < 1:129264/134‑1 (MQ=255) cATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTTGCGCCCATTGGGCG‑CGGGTTTTTTGTCAAAAATTCAATTTTGGCTTCGCTTTTTAGGATCGCGTGGCGGTGAAGTTAAAGACTac > 1:259523/1‑135 (MQ=255) tGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGGCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGCCTACACCAATGAc > 2:94712/1‑135 (MQ=255) aGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑GTTTTTTTGCCTAAAATTCAAGTAGGCGTTAGATTGTACGCATCGCATGCCGGGGAAGTTCATGACTAACCCAATGACCa > 1:212637/1‑135 (MQ=255) tGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGt < 3:36597/135‑1 (MQ=255) acgTGCTCTTTTGTTAAAAGGTGTATTTTTGGCGCCCTTTGGG‑GGGTTTTTTTTTGCATAAAATTCAAGTGGGGGTTCTATTGTCCGCATCGAATGCCGGTTAATTTCATGACTACACCAATGACCAGTCTCtt < 4:283750/132‑1 (MQ=255) aGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTTAAATTGAAGTATGCGTTAGCTTGTTAGCATCGCATGGCGGTGTACTTGATGAATAAACCAAAGACCAGTCTCttaa > 5:217001/1‑133 (MQ=255) aGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCGAAAATTAAAGTAGGCGTTCGATTGTACGCCTCGCCTGCCGGGGAAGTTCATGACGACACCAATGACCAGTCGCTTc > 5:221936/1‑134 (MQ=255) ttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑GTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACGACACCAATGACCAGTCTCTTCAATGTGAGCg > 7:215841/1‑135 (MQ=255) ttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCG‑CGTTTTTTTTGCCTAAAATTAAAGTATGCGTTCGATTGTTCGCATCGCATGCCGGTGAAGTTAATGACTTCACCAATGACCAGTCTCTTCAATGTGAGCg > 3:170394/1‑135 (MQ=255) tGGTAAAAGGTGGATATTTGGCGCCCATTGGGAGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCCTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCATTCTCTACAATGTGAGCGa > 6:291138/1‑135 (MQ=255) ggTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCg < 7:167920/133‑1 (MQ=255) aacaGGGGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGTCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTc < 5:178713/132‑1 (MQ=255) gTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGt < 5:197410/135‑1 (MQ=255) | ATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGT > KT2440/44169‑44401 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |