| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 3,061,793 | G→A | S592N (AGC→AAC) | qedA_2 → | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,061,793 | 0 | G | A | 95.8% | 78.1 / NA | 24 | S592N (AGC→AAC) | qedA_2 | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (17/6); minor base C (0/1); total (17/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACC > KT2440/3061661‑3061918 | tttCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg < 2:84808/135‑1 (MQ=255) tAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGc < 8:31041/135‑1 (MQ=255) cggcgCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGCCTTCAACCAGGGCGGCATGCTCTGGCCCTTCAAGTTGCCCAAGCAGTTGCCGCAAACGGCAAACGTCCAGCCATAACGCCTGCCTAc > 5:223907/1‑135 (MQ=255) cggcgCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTGAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCCAGCCATAACgcctgccg > 3:162636/1‑132 (MQ=255) gcgcGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGGCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCCTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTg < 7:253572/135‑1 (MQ=255) gCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTtgctg < 3:43525/135‑1 (MQ=255) gCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTtgctg < 7:41637/135‑1 (MQ=255) aggggCGGCGAAGTGGCCAAACGGGTCAAGGAATTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTTCCGCTTGCTGCCGc > 7:161731/2‑135 (MQ=255) cggcgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTCCTGCCGCTTGCTGCCGCTGATc > 7:193129/1‑135 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTTCAGCAAACGTCAAACGACAAGCCATAACGCCAGCCTACTGCCGCTTGCTCCCGGTGAACGGTGGCAAGCCGCCGTACTACCAAATGAcc > 5:125138/1‑132 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCCAGCGGCCCTACTACCAAATGACgag > 1:248916/1‑135 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCCACCGGCCCTACTACCAAATGaacag > 6:20766/1‑131 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACgag > 2:233706/1‑135 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACgag > 4:16802/1‑135 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACg > 3:79853/1‑133 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCCGTGGCAAGCGGCCCTACTACCaaaatacc > 6:189926/1‑128 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATAGGTGGCAAGCCGCCCTAATACCAAATGAcc > 4:43364/1‑132 (MQ=255) ggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGc > 4:207780/1‑135 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa > 7:169795/1‑44 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa < 8:169795/44‑1 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTAGcc > 7:16685/1‑135 (MQ=255) tGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa < 5:276000/41‑1 (MQ=255) tGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa > 6:276000/1‑41 (MQ=255) agcaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGcttctt > 4:281310/1‑135 (MQ=255) aaccGGCAACCGTAACGCCATAACGCGTGCCTCCTGCCGCTGGCGGCGGCTGTTCGGGGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAcc < 4:79853/132‑1 (MQ=255) | TTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACC > KT2440/3061661‑3061918 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |