Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	3,957,251	G→A	P133L (CCG→CTG)	dmlR_13 ←	HTH‑type transcriptional regulator DmlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,957,251	0	G	A	100.0%	192.1 / NA	55	P133L (CCG→CTG)	dmlR_13	HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/31);  total (24/31)

GGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGG  >  KT2440/3957126‑3957381                                                                                                                              |                                                                                                                                   gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGt                                                                                                                           >  3:111620/1‑135 (MQ=255)   gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGCTCAGTTGCGAATCCGGCAGAGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                         >  7:279959/1‑135 (MQ=255)   gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCCCGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                         >  1:105128/1‑135 (MQ=255)   gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                         >  7:102997/1‑135 (MQ=255)   gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                         >  2:157050/1‑135 (MQ=255)   gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                         >  6:152157/1‑135 (MQ=255)     gtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTgcg                                                                                                                       >  7:100810/1‑135 (MQ=255)     gtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTgcg                                                                                                                       >  5:51105/1‑135 (MQ=255)           gTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCg                                                                                                                  >  2:54163/1‑134 (MQ=255)                 gggggCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGt                                                                                                           <  4:111620/135‑1 (MQ=255)                  ggggCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGGCGGGGTTGCGCTGCGACAGCGTc                                                                                                          <  6:61305/135‑1 (MQ=255)                       cgcgCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTc                                                                                                     >  7:268713/1‑135 (MQ=255)                       cgcgCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTc                                                                                                     <  7:57646/135‑1 (MQ=255)                         cgcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGa                                                                                                   <  4:117286/135‑1 (MQ=255)                          gcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                                  <  6:51105/135‑1 (MQ=255)                            aGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATca                                                                                                >  7:146747/1‑135 (MQ=255)                               ggATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcacca                                                                                             <  7:246907/135‑1 (MQ=255)                               ggATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcacca                                                                                             <  2:176389/135‑1 (MQ=255)                                    cTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTcc                                                                                        >  8:277926/1‑135 (MQ=255)                                        aaaTATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                                      <  5:184142/133‑1 (MQ=255)                                         aaTATTGCCCAGCGGCACAGCGATCAGTTGCGACTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgggg                                                                                    >  2:205221/1‑133 (MQ=255)                                          atatTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTaa                                                                                  <  8:180138/135‑1 (MQ=255)                                                  cAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtgtt                                                                          >  4:40330/1‑135 (MQ=255)                                                    gCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtgttgt                                                                        <  2:65264/135‑1 (MQ=255)                                                    gCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtgttgt                                                                        <  6:135741/135‑1 (MQ=255)                                                        cacaGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGcc                                                                    >  3:165986/1‑135 (MQ=255)                                                                     tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                                       <  6:243974/135‑1 (MQ=255)                                                                        gtaTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATc                                                    <  3:156883/133‑1 (MQ=255)                                                                          aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCTACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                  <  7:251378/135‑1 (MQ=255)                                                                          aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                  <  6:93395/135‑1 (MQ=255)                                                                               gCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACa                                             <  2:211793/135‑1 (MQ=255)                                                                               gCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACa                                             <  2:115671/135‑1 (MQ=255)                                                                                      aCCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                                      <  8:266217/87‑1 (MQ=255)                                                                                      aCCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                                      >  7:266217/1‑87 (MQ=255)                                                                                           ccACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGt                                 >  5:251956/1‑135 (MQ=255)                                                                                              cGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                              <  1:89677/135‑1 (MQ=255)                                                                                              cGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                              <  1:229910/135‑1 (MQ=255)                                                                                              cGGTGATCACCACGTCATGCCCTGCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                              <  8:70301/135‑1 (MQ=255)                                                                                                gTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                                       >  8:134865/1‑108 (MQ=255)                                                                                                gTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGACGGCCACCAGCGGc                                                       <  7:134865/108‑1 (MQ=255)                                                                                                    tCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGt                        <  4:49969/135‑1 (MQ=255)                                                                                                      accacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTCTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTg                       >  4:224600/1‑134 (MQ=255)                                                                                                       ccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGAAACAGCCCCATGCCGTGCGGGCAGTGc                      >  4:136949/1‑134 (MQ=255)                                                                                                              aTGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAc                  >  7:164904/1‑131 (MQ=255)                                                                                                                gCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                  <  1:67915/97‑1 (MQ=255)                                                                                                                gCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                  >  2:67915/1‑97 (MQ=255)                                                                                                                   cTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc         <  8:268713/135‑1 (MQ=255)                                                                                                                   cTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc         <  1:157050/135‑1 (MQ=255)                                                                                                                   cTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc         <  4:165986/135‑1 (MQ=255)                                                                                                                   cTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc         <  3:40330/135‑1 (MQ=255)                                                                                                                   cTTCGGCCAGCAGGTCGGGGGTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc         <  3:261219/135‑1 (MQ=255)                                                                                                                   cTTCGGCCAGCAGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGAGCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc         <  2:38805/135‑1 (MQ=255)                                                                                                                   cTTCGGCCAGCAGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCAATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc         <  6:200441/135‑1 (MQ=255)                                                                                                                        gCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGa    >  7:265269/1‑135 (MQ=255)                                                                                                                        gCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGa    >  5:277405/1‑135 (MQ=255)                                                                                                                          cagcagGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAgg  <  6:251956/135‑1 (MQ=255)                                                                                                                              |                                                                                                                                   GGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGG  >  KT2440/3957126‑3957381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑