Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440237,8820AG29.5% 54.3 / 16.4 53T171A (ACT→GCT) CJ019_00211hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (16/15);  new base G (13/0);  total (33/20)
Fisher's exact test for biased strand distribution p-value = 1.53e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCC  >  KT2440/237756‑238013
                                                                                                                              |                                                                                                                                   
gCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGACAGCGCGACCCCAGCCGCCAAGCCTGCGGCCAAACCAGCTCCCAACCCCACAGCTGCCGCCaa                                                                                                                           >  6:455341/1‑137 (MQ=17)
  ccTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGc                                                                                                                         >  6:471229/1‑137 (MQ=255)
   cTTGATCAACGTGCGGCCAAAACCGCAGCACACGCAACCGCTGCAGCCAAGCCTGCGGCCAAACCAGCTGCCAGCGCGACCGCTGCCGCCAAGCCTGCTGCCAAACCAGCTGCCAAGACGACAACTTCAGCCACGcc                                                                                                                        >  8:52932/1‑137 (MQ=17)
        tCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCCGCTGCCAAGGCGACCACTGCCGCCACGCCTGccg                                                                                                                   >  2:142025/1‑135 (MQ=255)
        tCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCTCTGCCGCCAAGCCTGCgg                                                                                                                   >  5:92163/1‑137 (MQ=255)
        tCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCgg                                                                                                                   >  2:2075/1‑137 (MQ=255)
        tCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCgg                                                                                                                   >  7:442804/1‑137 (MQ=255)
        tCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCAAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTCCCGCAAAGCCTGCgg                                                                                                                   >  7:144244/1‑137 (MQ=255)
        tCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCACGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACTACATCTGCCGCCAAGCCTGCgg                                                                                                                   >  4:116122/1‑137 (MQ=255)
        tCAACGTGCGGCCAAACCGGCAGACAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGCACGCTGCCGCCAAGCCTGCCGCCAAACCCGCTGCCAAGACCACCGCAGCCGCCAAGCCTTCgc                                                                                                                   >  6:3720/1‑136 (MQ=14)
             gTGCGGCCAAACCGTCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCTCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTTCCGCCAAGCCTTCGGCCaaa                                                                                                              <  7:351386/137‑1 (MQ=255)
              tGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCACGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCACGCCTGCGGCCAAAc                                                                                                             >  3:11509/1‑137 (MQ=255)
               gCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAAcc                                                                                                            >  3:64104/1‑137 (MQ=255)
               gCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAAcc                                                                                                            >  5:122472/1‑137 (MQ=255)
               gCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCACGGCGCCCGCTGCCGCCACGCCTGCGGCCAAACCAGCTGCCAAGGCGACAGCTGCCGCCAATCCTGCGGCCAAAcc                                                                                                            >  2:127437/1‑137 (MQ=11)
               gCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCACGGCGACAGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGCCGACCCCTGCCGCCAATCCTGCGGCCAAGcc                                                                                                            >  1:304385/1‑137 (MQ=14)
                 ggCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCgg                                                                                                          >  1:45368/1‑137 (MQ=255)
                   ccAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGCCCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGCCCCCTGCCGCCACGCCTGCGGCCAAACCGGCt                                                                                                        >  6:391695/1‑137 (MQ=255)
                         cGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCGCTGCCGCCCAGCCTGCGGCCAACACGGCTGCCAAg                                                                                                  >  7:154836/1‑137 (MQ=255)
                              gCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGAc                                                                                             <  6:427311/137‑1 (MQ=255)
                               ccAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGAcc                                                                                            <  1:27768/137‑1 (MQ=255)
                               ccAAGCCCACCGCCGCTGCCAACCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCGGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGCCGACCCCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGAcc                                                                                            <  2:365790/137‑1 (MQ=14)
                                           ctgctgCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCaaa                                                                                <  3:281228/137‑1 (MQ=255)
                                              ctgcCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCt                                                                             <  2:167492/137‑1 (MQ=255)
                                                        tGCGGCCAAACCAGCTGCCAGGGCGCCCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAGGTCGCCCGCTGCCGCCAAGCCTGCGGCCACACCCGCTGCCAAGGCGACCGCTGCCGCCACCCCTGCGGccgcac                                                                   >  5:6125/1‑133 (MQ=11)
                                                        tGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAAc                                                                   <  4:64104/137‑1 (MQ=255)
                                                        ggCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAAc                                                                   <  5:42188/136‑1 (MQ=255)
                                                            gCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGCCGCCAGCTGCCGCCAAGCCTGCGGCCACCCCGGCTGCCAAGGCGCCCGCTGCCGCCACGCCTGCGGCCAAACCGGc                                                               >  1:326043/1‑137 (MQ=11)
                                                             ccAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAACCCAGCTGCCAAGGCGCCCGCTGCCGCCACGCCAGCGCCCCAACCGGCTGCCAAGGCGCCCGCTGCAGCCCCGCCAGCGGCCCACCCGGCt                                                              >  6:370162/1‑137 (MQ=11)
                                                             ccAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGCCGACCGCTGCCGCCAAGCCTGCGGCCAACCCGGCTGCCAAGGCGACGGCTGCCGCCACGCCTGCGGCCAAACCGGca                                                              >  6:303992/1‑136 (MQ=11)
                                                             ccAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCACACCGGCTGCCAAGGCGCCCGCTGCCGCCACACCTGCGGCCAAACCGGca                                                              >  5:235489/1‑136 (MQ=255)
                                                                   cAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGCCAGCTGCCGCCAAGCCTGCGGCCAACCCGGCTGCaaac                                                        >  2:340912/1‑136 (MQ=11)
                                                                        gCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGAc                                                   <  6:152027/137‑1 (MQ=255)
                                                                        gCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGAc                                                   <  6:122472/137‑1 (MQ=255)
                                                                         ccatgtcGACCGCTGCCGCCAAGCCTGCGGCCAAACCCGCGGCCAATACTACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCGGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGAcc                                                  <  5:466360/131‑1 (MQ=14)
                                                                         ccAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACAGCTGCCGCCACTCCTGCGGACAAACCGGCAGCCAAGGCGAcc                                                  >  6:62442/1‑137 (MQ=11)
                                                                         ccAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGAcc                                                  <  3:402827/137‑1 (MQ=255)
                                                                          cAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCg                                                 >  1:288357/1‑137 (MQ=255)
                                                                               cGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTgcc                                            <  8:36354/137‑1 (MQ=255)
                                                                                    gCTGCCGCCAAGCCTGCGGCCAAACCACCTGCCAAGCCGCCCGCTGCCGCCAAGCCTGCGGCCAACCCGGCTGCCACGGCGACCGCTGCCGCCACACCAGCGGCCACACCGGCTGCCAAGGCGACCGCTCCCGCCaa                                       >  2:414771/1‑137 (MQ=11)
                                                                                           ccAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAACCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCg                                >  7:462426/1‑137 (MQ=255)
                                                                                                ccTGCTGCAAAACCAGCTGCCAAGCCGACCCCTGACGCCCAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAACCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCaa                           <  2:306091/137‑1 (MQ=11)
                                                                                                  tGCGGCCAAACCCGATGCCAAGACCACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAAc                         <  8:7798/137‑1 (MQ=255)
                                                                                                     ggCCAAACCAGCTGCCAAGCCGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGCCCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGCCCGCTGCCGCCAAGCCTGCGGCCAAACCgg                      >  8:89248/1‑137 (MQ=255)
                                                                                                       ccAAACCAGCTGCCAAGACGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTgcc                                                                                      >  8:370880/1‑71 (MQ=21)
                                                                                                       ccAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAACGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCt                    <  7:447139/137‑1 (MQ=255)
                                                                                                       ccAAACCAGCTGCCAAGACCACCCCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCGGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCt                    <  7:347957/137‑1 (MQ=255)
                                                                                                               ccTGCGAAGCAGACCCCTGCCCCCAAGCCTGCGGCCAAACCGGCTGACCAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGc            <  4:69590/136‑1 (MQ=11)
                                                                                                                 tGCCAAGCCGACCCCTGCCGCCAAGCCTCCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGa          <  2:288357/137‑1 (MQ=255)
                                                                                                                   ccAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAACCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGAcc        >  8:429861/1‑137 (MQ=37)
                                                                                                                   ccAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGAAGCCAAGGCGACCGCTGCCGCCAAGACTGCGGCCAAACCGGCTGCCAAGGCGccc        >  1:365388/1‑137 (MQ=255)
                                                                                                                   ccAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACAGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGAcc        >  5:462280/1‑137 (MQ=255)
                                                                                                                         cgacCACTGCCGCCACGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTgcc  <  3:227222/137‑1 (MQ=37)
                                                                                                                              |                                                                                                                                   
GCCCTTGATCAACGTGCGGCCAAACCGGCAGCCAAGGCAACCGCTGCTGCCAAGCCTGCGGCCAAACCAGCTGCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCC  >  KT2440/237756‑238013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑