Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440434,3760G.30.0% 68.2 / 30.8 30coding (207/1173 nt)bioF8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base G (13/8);  new base . (5/4);  total (18/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCC  >  KT2440/434245‑434508
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aGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGGCCGCGGTTGGTGGGCGTGGGGCGCAATGGCTACCGGGGCCGGGCCAACCACCCCGAGGTGATAGCCCCCGGGCAGGCCGGCGCCGCCGGCGGGGGTGTgggggg                                                                                                                                 >  2:154664/1‑137 (MQ=255)
aGCCCGCAAGGGCCGGAAGTGGTGGACGACGGCCAGCGGGTGCTGGCCTTCGGCAGCAATGACGACCTGGGCCTGGCCAACCCCCCCGCGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCGGGGGTGTgggggg                                                                                                                                 >  3:182442/1‑137 (MQ=255)
     gCAAGGGCCGGAAGTGGTGGTCGACGGCCCGCGGGTTGTGGGCGTGTGGCGCAATTACTAACAGGGGCTGGCCCACCCCCCCCCGGTGATAGCCCCCGGGCCCGCCCGCGCCGCGCCCGgggggggggggggggggg                                                                                                                            >  2:319042/1‑137 (MQ=255)
       aaGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTTGGGGGCGTCTGCCGCAATGACAACCAGGGCCCGGCCCACCACCCCCAGGTGATAGCCCCCGGGCCGGCCGGCGCCGAGCGCTGGGGGGTggggggggggggg                                                                                                                          >  4:204962/1‑135 (MQ=255)
        aGGGCCGGAAGTGGTGGTCGACGGCCCGCGGTTGCTGGGCGTCTGCCGCAATGCATACCTGGGCCGGGCCAACCACCCCGCGGTGATAGCCCCCGGGCAGGCCGGCGCCGCGCGCGgggggggggggggggggggcg                                                                                                                         >  1:341467/1‑134 (MQ=255)
                                  cgCGGGTGGTGGCCTTGTGCAGCAAACCCACCCGGGGCCGGGCCAACCCCCCCGCGGGGCTCGCCGCCGGGCCGGCCGGCCCCGCGGCGGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCAcc                                                                                               <  8:190110/136‑1 (MQ=255)
                                        tGCTGGCCTTCTGCAGCAATGACAACCTGGGCCGGGCCAACCCCCCCGCGGTGATCGCCGCCGGGCCGGCCGGCGCCGCGCGCTGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAc                                                                                         <  1:104014/137‑1 (MQ=255)
                                             gCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCCCCCCGCACCATCa                                                                                    >  4:417260/1‑137 (MQ=255)
                                               cTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAgg                                                                                  >  6:115809/1‑137 (MQ=255)
                                                      cgcaATTACACCCGGGGCCTGGCCAACACCCCCGTGTGTATGCCCGGCTGGCCGGGCGGGGCCGCGCGGGGGGGGTGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAaga                                                                           <  5:284047/136‑1 (MQ=255)
                                                      accaATCACTACCGGGGCCGGGCCAACCCCCCCGCGGTGCGCGCCGCCGGGCCGGCGGGCGCCGCGGGCTGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAaga                                                                           <  7:290327/135‑1 (MQ=255)
                                                              cccccggggCCTGGCCCACCCCCCCCGGGGGTTCGCCGCCGGGCCGGCCGGGGCCGCCCGGGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTgg                                                                   <  8:108447/131‑1 (MQ=255)
                                                                aCCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCt                                                                 >  5:257876/1‑137 (MQ=255)
                                                                        cTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCGCGCCCCTGGTAGTTGGCCACAGCACCCCGCCCCATCAGGTTGAAGAGGCACAGGCTGAAcaacc                                                         >  7:453540/1‑133 (MQ=255)
                                                                         tGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCAcc                                                        >  7:59168/1‑137 (MQ=255)
                                                                              aaCCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCCCCGGGTAGTTGGCCACACCACCCCGCCCCTTCAGGTTGAAGAGGCACTGGCTGAACTCACCGgccg                                                   >  3:301398/1‑137 (MQ=255)
                                                                                   ccccGCGGGGCTCCCCGCCGGGCCGGCCGGGGCCGCGCGGGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGccgcccgc                                              <  5:332164/137‑1 (MQ=255)
                                                                                   ccccGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACAATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCcgcg                                             >  8:248759/1‑137 (MQ=255)
                                                                                        aGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTgg                                                                                                                >  5:253689/1‑65 (MQ=255)
                                                                                        aGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTgg                                                                                                                <  6:253689/65‑1 (MQ=255)
                                                                                         ggTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCGGCGCCCCTGGTAGTTGGCCACAGCCCCCCGCACCATCAGGTTGAAGCGGCACTGGCGGACCACACCGGCCGCCCGCGCGccc                                        >  8:402267/1‑137 (MQ=255)
                                                                                           tGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCCCCTGGTTGTTGGCCACCGCCCCCCGCCCCTTCAGGTTGAAGTGGCACTGGCGGAACTACCCGGCCGCCCGCGCGcccgg                                      >  7:431332/1‑135 (MQ=255)
                                                                                                gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCCCCTGGTAGTTGGCCACAGCACCCCGCCCCATACGTTGGAAGAGGCACTGGCTGCACTCACCGGCCGCCCGCGCGCCCTgttgtt                                 >  7:106485/1‑137 (MQ=255)
                                                                                                gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCCCCGGGTAGTTGGCCACAGCCCCCCGCCCCATCAGGTTGATGAGGCACTGGCGTAACTCACCGGCCGCCCGCGCGCCCGgttttt                                 >  8:268084/1‑137 (MQ=255)
                                                                                                gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTGGAAGAGGCCCTGGCTGAACTCACCGGCCGCCCCCGGGCCCGgttgtt                                 >  3:34953/1‑137 (MQ=255)
                                                                                                gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTtc                                >  8:368399/1‑137 (MQ=255)
                                                                                                 ccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCCCCTGGTAGTTGGCCACCGCCCCCCGCACCATCAGGTTGAAGAGGCACTGGCTTAACTCACCGGCCGCCCCCGCGCCCggttgtgc                                >  2:445928/1‑135 (MQ=255)
                                                                                                 ccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTtct                               >  5:349005/1‑137 (MQ=255)
                                                                                                  cgccGGGCAGGCCGGTGGCGACCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAGCTTGATGTTGAAGAGGCACTGGCTGTACTCACCGGCCGCCCGCGCGCCCTGTTGTtctc                              <  6:20784/137‑1 (MQ=255)
                                                                                                   gccTGGCTGGCCGGCGCCGTGGGGTGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTtctc                              <  6:257876/137‑1 (MQ=255)
                                                                                                   gccTGGCCGGCCGGCGCCGAGGGGTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTtctc                              <  3:122572/137‑1 (MQ=255)
                                                                                                        gccggCGGGGGCCGGGCGGGGGGGGGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCgg                        <  1:214920/134‑1 (MQ=255)
                                                                                                              cGGCGCCGAGCGCGGGGGGGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTatat                  <  6:426129/137‑1 (MQ=255)
                                                                                                                              ggTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCGGTTGTTCTCCACCGGCTATATGGCCAATCTGGgcgc   >  3:22504/1‑137 (MQ=255)
                                                                                                                              ggTGT‑GGGGGGGGGGCCTCGCCCCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCAATGGCTGAACTCACCGGCCGCCCGCGCGCCCGGTTGTTCTCCACCGGCTATATGCGCAATCTGGGCGcc  >  5:274024/1‑137 (MQ=255)
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AGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCC  >  KT2440/434245‑434508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑