Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,123,2380CA55.0% 9.6 / 32.8 41intergenic (‑240/+64)gcvH_1/CJ019_01062Glycine cleavage system H protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (11/7);  new base A (22/0);  total (34/7)
Fisher's exact test for biased strand distribution p-value = 1.71e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCA  >  KT2440/1123108‑1123366
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aGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTGTGAGCAGCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTacg                                                                                                                            <  4:332469/137‑1 (MQ=255)
       gTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGc                                                                                                                     >  3:148395/1‑137 (MQ=255)
         aaaTTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCAAAGACGGAGCGa                                                                                                                   >  3:392534/1‑137 (MQ=255)
              tACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACCTTGCGAAAACg                                                                                                              >  5:199522/1‑137 (MQ=255)
                    gaAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCAACGACGTATCGTAAAAGaaaaaa                                                                                                        >  3:302423/1‑132 (MQ=255)
                    caaGCCGTATTTCTTGGGGGGAATGATCACGTAACCGGGGCCTCTGAGCGCAAAAGGGTTCCCGCATCAAACCCTTGTGCACCGACGGCGAGGCGGCTGTGTATCGTTTTCCCTACGACGTAGCTAAAACGATACAt                                                                                                        <  8:447288/136‑1 (MQ=255)
                        ccGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTTGCGAAAAAGGTTCATgtgg                                                                                                    >  5:163639/1‑137 (MQ=255)
                          gTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAACGATACATgtgggg                                                                                                  >  8:309788/1‑135 (MQ=255)
                                attGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGa                                                                                            >  6:236659/1‑137 (MQ=255)
                                    tgtgCAATCATATCGTACCCGGGGCCTCTCACCCGAAAGGGGTTTGCCCAAAAACCCCTTGGGCCCCGACGGCGAGGAGGCTTTGTATGGTTTGCCCTACGACTTAGCTAAAACGATACATGTGGTGCCTGGACTgg                                                                                        <  8:255498/137‑1 (MQ=255)
                                     gtgtAATGATAGCGTTACAGGGCCCTCGGATAGTAAACGGGTTTGACCACCAAACCTTTGTGCACCGCCGGCGCGGAGGCTGGGTATCGTTTCCGCTACGCCGTAGCTAAAACGATACATGTGGTGCCTGGACTGGc                                                                                       <  8:312456/137‑1 (MQ=255)
                                      tgGAATTATATCGTTCCTGGTCCCGCTGAGAGGAAAAGGGTTTGCGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCTAAAACGATACATGTGGTGCCTGGACTGGCg                                                                                      <  6:425842/137‑1 (MQ=255)
                                      tgGAATGATATCGTTACCGGTGACTCTGAGAGTAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACCAAGCGCAAACGATACAAGTTGTGCCAGGCCTTGCg                                                                                      >  2:247133/1‑137 (MQ=255)
                                         aaTGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGCCGGAGCGCAAACGCTACAAGTGGTGGCGGGTCTGGGGAgg                                                                                   >  3:406864/1‑137 (MQ=255)
                                          aTGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTTCGACGTAGCGAAAACGCTACATGTGGTGCCTGGACTGGCGAggg                                                                                  >  1:337902/1‑136 (MQ=255)
                                            tatatCTTAACAGGGCCCTCTGATAGTAAAAGGGTTTGACAATCAAACCTTTGTGCACCGACGGCGAGGTGGCTGTGTATGGTTTTCGCTACGCCGTAGCTAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGc                                                                                <  8:194195/136‑1 (MQ=255)
                                            tatagggTTACTGGGGACTGTGAGTGTAAACGGGTTTGCCCCACAAACCCTTGTCCCCCGACGGCGAGGCGGCTGTTTCTCGTTTTCGCTCCGCGGTAGCTAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGc                                                                                <  4:385481/131‑1 (MQ=255)
                                                 cGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGCCGTAGCGAAAACGATACATGTGGTGGCGGGGCTGGCGCGGTGCCAAtt                                                                           >  7:6408/1‑136 (MQ=255)
                                                      cAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGGGAAAACCATACATGTGGTGCCTGGGCTTGCGAGGTGCCAATGGCCtt                                                                      >  7:194195/1‑137 (MQ=255)
                                                      cAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGcccg                                                                      >  5:425842/1‑135 (MQ=255)
                                                      cAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCGACGACGGAGCGAAAACGATAAATGTGTTGGCTGGACTGGGGAGGTGCCAAATGCCtt                                                                      >  4:40000/1‑137 (MQ=255)
                                                               ctGAGAGTAAAAGGGTTTGAGAATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGGAACCAAGTAGCGAAAACGATACATGTGGTGGCTGGAATGGCGAGGTGACAATGGCCTTCGGTcctgg                                                             >  2:279940/1‑133 (MQ=255)
                                                               ctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTGGGTAAGCCCTAGCGAAAAAGATACCTGTGGTGGCGGGGCTGGCGCGGTGGCAATTGCCGTAGGtggtct                                                             >  1:94231/1‑132 (MQ=255)
                                                               ctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtct                                                             >  6:132316/1‑137 (MQ=255)
                                                               ctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtct                                                             >  1:275918/1‑137 (MQ=255)
                                                               ctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAACGATAAATGGGGTGCGTGGACGTGCGAGGTGGCAATGGCCTTCGGGCAgct                                                             >  5:246901/1‑137 (MQ=255)
                                                               ctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAAAGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtct                                                             >  6:153197/1‑137 (MQ=255)
                                                               ctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTACGACGGAGCGCAAAAGAATCAATGGGGGCCTGGACTGGCGCGGTGGCCATTGGCTTAGGGCAtat                                                             >  3:11142/1‑135 (MQ=255)
                                                               ctGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGCTAAGCCGTAGCGAAAACGATACATGTGGTGCCGGGACTGGCGAGGTGCCAATGGCCGTCGGTCAtct                                                             >  3:270599/1‑137 (MQ=255)
                                                                       aaaaGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGGTACGGCGTTGGGGAAAAGATACATGTGGTGGCGGGGCGGGGGGGGTGGCAATGGCCGTGGGGGATCTCACattga                                                     >  7:189035/1‑135 (MQ=255)
                                                                            ggTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGGCGTAGGCAAAAAGGTACATGTGGTGGCGGGCCGGGGGAGGGGCCAATGGGCGTGGGTGATCGCCCGTTcagcagg                                                >  8:420225/1‑136 (MQ=255)
                                                                                                  gCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTAAGCCGGAGCGAAAACCGTAAATGTGGTGCCTGGACTGGGGCGGTGCCAATGGCCGTCGGTGATGTGACTTTGAGCAGATGCTCCCGCGAAAACGCGCCgg                          >  4:65528/1‑137 (MQ=255)
                                                                                                  gCACCGACGGCGAGGAGGCTGTGTATCGTTTTAGGTACGACGTATCGTAAACGATAAATTTGGTGGCTGGTCTTGCGGGGTGGCAATTGCCTTAGGTGATCTAAATTTCAGCAGATTATCCAGCGAAAACGCGCCgg                          >  2:398708/1‑137 (MQ=255)
                                                                                                  gCACCGACGGCGAGGAGGCTGTGTATAGTTTTAACAACCCCGGAGCGAAAAACATACATGTTGTGCCGTGACAGGCGGGGGGCAAAATGCCATAGGTGAAATAACTTTAAGCAGCTGAAACCGCCAAAACACGCCgc                          >  8:297309/1‑136 (MQ=255)
                                                                                                         cGGCGAGGAGGCTGTGTATCGTTTTCGCTAAGCCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTAATCTCACTTTGAGCAGATGATCCAGCGAAAACGCCCCCGCGCCTCg                   >  5:333669/1‑137 (MQ=255)
                                                                                                         cGGCGAGGAGGCTGTGTATCGTTTTATCTAAGACGGTGCGAAAAAGATAAAATTGGTGGCGGGACTGGCGCTGTGGCCAAGGCCGTCGGTGATATCACGTTTACCCGATTATCCAGCGAAAAAGCGCCCGCGccgcc                   >  4:6019/1‑134 (MQ=255)
                                                                                                         cGGAGAGGAGGCTGTGTATCGTTTTAGCTACGACGTAGCGAAAACGATAAATGTGGTGCCAGGACTGGGGAGGTTCCAAAGGCCTTCGGTCATAGGACATTAAGCAAATGATCCAACGAAAACGCGCCGGCCCCTCg                   >  4:47962/1‑137 (MQ=255)
                                                                                                           gCGAGGAGGCTGTGTATCGTTTTAGCTACGGCGTAGCGCAAACGATACATGTTGTGCCTGGACTGGCGAGGTGCCAATTGGCGTCGGTGAAATCACGTTAAGCAGATGATCCAGCGAAAACGCGGCGGCCCCGCggg                 >  8:413729/1‑137 (MQ=255)
                                                                                                              aggaggCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCaa              >  7:129815/1‑137 (MQ=255)
                                                                                                                 aggCTGTGTATCGTTTTCGCTAAGACGTAGCGAAAAAGATACATGGGGTGGCTTGACTGGCGAGGTGCCAATGGCCTGCGGGCATGTGACTTTCAGCAGCTGGTACCGCGAAAACCCGCCGGCGCCGGGGGCACCCa           >  8:461300/1‑137 (MQ=255)
                                                                                                                          aTCGTTTTCGCTACGACATAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAgcagca  >  2:88542/1‑137 (MQ=255)
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AGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCA  >  KT2440/1123108‑1123366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑