Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,138,3670TG26.3% 95.4 / 10.4 57F240V (TTT→GTT) rbsC_2Ribose import permease protein RbsC
Reads supporting (aligned to +/- strand):  ref base T (20/22);  new base G (15/0);  total (35/22)
Fisher's exact test for biased strand distribution p-value = 1.61e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGGCAAC  >  KT2440/3138238‑3138503
                                                                                                                                 |                                                                                                                                         
acGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTc                                                                                                                                    >  7:208096/1‑137 (MQ=255)
   ccATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCAGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTAGCTGATCTGCGGTCTGTGCGTCTGCGTGGCCGCGTTTTGTTcggc                                                                                                                                 <  5:250070/137‑1 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTgg                                                                                                                          >  7:304459/1‑137 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTgg                                                                                                                          <  5:420153/137‑1 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTgg                                                                                                                          >  4:25771/1‑137 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTgg                                                                                                                          <  3:266732/137‑1 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTAGGCGGCCggg                                                                                                                          >  2:115053/1‑137 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGGTAGGCGGGCTgg                                                                                                                          >  6:380625/1‑137 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGGTAGGCGGGCTgg                                                                                                                          >  3:287983/1‑137 (MQ=255)
                 tCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTGTTGTTTGGGGGCCTGGGTCAGCg                                                                                                                   >  3:114510/1‑137 (MQ=255)
                        gCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGCCGGGTTCAGCGGCTGcac                                                                                                            >  5:69948/1‑137 (MQ=255)
                        gCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGcac                                                                                                            <  7:228047/137‑1 (MQ=255)
                        gCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGcac                                                                                                            >  5:414745/1‑137 (MQ=255)
                        gCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGcac                                                                                                            <  3:439495/137‑1 (MQ=255)
                            tCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCaccacc                                                                                                        >  8:368528/1‑137 (MQ=255)
                             cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCTGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTGTTGTAGGCGGGCTGGTTCACCGGCaccaccaccc                                                                                                       >  8:338640/1‑136 (MQ=255)
                             cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTACGCGGCCTGGTTCAGCGGCCGCaccaccc                                                                                                       >  7:387091/1‑136 (MQ=255)
                               gagaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTGTCGGCGGCCTGGTTCAGCGGCAGCACCCCCggg                                                                                                     >  4:261338/1‑136 (MQ=255)
                                agaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTAGGCGGGCTGGTTAAGCGGCCGCaccacccggt                                                                                                    >  1:141221/1‑133 (MQ=255)
                                   aTACGCGGAGGCGGCTAATGCCGCTGGGCTGTCGGTGCGTGGCTACTTGTTCGCGAGCTACCTGATATGCGGTCTGTGCTGTAGCCTGGCCGAGTTTTGTTCGGCGGCCTGGGTCAGCGGCAGCACCACCGGTTcag                                                                                                 <  8:171601/137‑3 (MQ=255)
                                   aTACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCg                                                                                                 <  6:406378/137‑1 (MQ=255)
                                   aTACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCg                                                                                                 <  6:453140/137‑1 (MQ=255)
                                   aTACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCg                                                                                                 <  4:409524/137‑1 (MQ=255)
                                   aTACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCg                                                                                                 <  8:208096/137‑1 (MQ=255)
                                       ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGGTCGGCGGCCGGGTGGAGCGGCAGCCCCACCGGTTCCggggg                                                                                             >  6:428189/1‑134 (MQ=255)
                                       ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCTGGTTCAGCGGCAGCACCCCCGGTTCCGGCgc                                                                                             >  7:346576/1‑136 (MQ=255)
                                       ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGGGGCCGGGGTGAGCGGCCGCACCACCCGGTCCGGCgc                                                                                             >  3:152919/1‑136 (MQ=255)
                                       acGGAGGCGGCTAATGCAGCTCGCCTGTCGGTGCCTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTAGCCCCGGCAGGTACAGCAGCACCCCCACCCGATCCGGcca                                                                                             >  4:386536/2‑135 (MQ=255)
                                         ggAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCgaga                                                                                           >  5:201154/1‑137 (MQ=255)
                                         ggAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGATACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTAGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCgagt                                                                                           >  7:357932/1‑136 (MQ=255)
                                           aGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtg                                                                                         <  4:119011/137‑1 (MQ=255)
                                             gcggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGCCGGGTTCAGCGGCAGCACCACCCGGTCCGGGGCGAttgt                                                                                       >  1:108582/1‑134 (MQ=255)
                                             gcggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtgct                                                                                       >  2:71843/1‑137 (MQ=255)
                                             gcggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCGGGTTCAGCGGCAGCCCCCCCGGTTCCCGCGCGAttct                                                                                       >  1:287823/1‑137 (MQ=255)
                                                   aaTGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTGTGTTAGGCGGCCGGGTTCAGCGGCAGCACCACCCGGTCCGGCGAGATGCTGCGGTc                                                                                 >  8:285352/1‑137 (MQ=255)
                                                    aTGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTcc                                                                                <  3:25771/137‑1 (MQ=255)
                                                    aTGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTcc                                                                                <  1:171772/137‑1 (MQ=255)
                                                           tGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑tggtgg                                                                        <  7:186054/137‑1 (MQ=255)
                                                             gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGGTAGGCGGCCTGGTTCAGCGGCACCACCACCGGTTCCCGCGAGATGGTGCTGTCCG‑TGGTGGcc                                                                      >  8:110093/1‑137 (MQ=255)
                                                             gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCGGGTTCAGCGGCCGCCCCACCGGTTCCGGCGAGATGGTGCTGTCCG‑gggggggc                                                                      >  4:249644/1‑135 (MQ=255)
                                                             gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTAGGCGGGCGGGTTGAGCGGCCGCACCCCCGGGTCCGGCGCGATGCTGCTGTGCG‑tggtgggc                                                                      >  7:221087/1‑135 (MQ=255)
                                                               cTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCAt                                                                    <  7:441442/137‑1 (MQ=255)
                                                               cTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCAt                                                                    <  7:443356/137‑1 (MQ=255)
                                                               cTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCAt                                                                    <  4:41611/137‑1 (MQ=255)
                                                               cTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCAt                                                                    <  8:304459/137‑1 (MQ=255)
                                                               cTGTCGGTGCGGGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCAt                                                                    <  4:269010/137‑1 (MQ=255)
                                                                           ggCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCATCGCTTTTCTTgg                                                        <  6:452874/137‑1 (MQ=255)
                                                                               aCGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCGGGTTCAGCGGCGCCCCCCCCGGGTGCGGCGAGATGCTGCTGTGCG‑GGGGGGGCCTCGCGGTTGTTgggggg                                                    >  7:214418/1‑135 (MQ=255)
                                                                                   gtTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCCCCACCGGGTCCGGCGAGATGCTTCTGTCCG‑TGGTGGCCATCGCGTTTCTTGGGGTggtgt                                                >  5:214354/1‑133 (MQ=255)
                                                                                   gtTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTATTTTGCGGGCGGGGTAAGACGGAGCACCAACCGTTCCGCCGAGATTCATCAGTTAGTTGGGGGCCA‑GGCTTTTGTTGGGGTGAtat                                                >  2:209379/1‑135 (MQ=255)
                                                                                         cAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTAGGCGGGCTGGTTCAGCGGCAGCACCACCGGGTCCGGGCAGATTCTGCTTTCCG‑TGGTGGCCCTCGCTTTTATTGGGGTTATATTCTgcc                                          >  7:305923/1‑137 (MQ=255)
                                                                                              aCCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGt                                     <  2:220168/137‑1 (MQ=255)
                                                                                               ccTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCTGGTTCAGCGGCCCCACCACCCGTTCCCGGGAGATGCTGCTGTCCG‑TGGTGGCCATCGCTTTTCTTGGGGGGATCTTCTCCCCGCCTc                                    >  8:302055/1‑137 (MQ=255)
                                                                                                  gATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGc                                 <  4:319323/137‑1 (MQ=255)
                                                                                                                gCGGCAGCCTGGCAGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATcggc                   >  2:214349/1‑137 (MQ=255)
                                                                                                                            ccGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCCCCGGTTGCGGCGAGATGCTGCTGTCCG‑TGGTGGCCATCGCTTTTCTTGGGGTGATATTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGctgctg       >  6:163604/1‑137 (MQ=255)
                                                                                                                            ccGCGGTTTGTTAGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTGCGGGGTGATGGTGCTGTGCG‑TGGTGGCCCTCGCTTTTGTTGGGGTGTTCTTCTCCCAGCGGCTGCAGCCGAGCCTCGGCGGCACGctggtg       >  7:201508/1‑137 (MQ=255)
                                                                                                                                 ttttGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGGCAAc  <  2:225321/137‑1 (MQ=255)
                                                                                                                                 ttttGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGGCAAc  <  4:113095/137‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                         
ACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCG‑TGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGGCAAC  >  KT2440/3138238‑3138503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑