Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,350,3630CG30.6% 84.2 / 11.8 53F29L (TTC→TTG) CJ019_04090hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (9/25);  new base G (15/0);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 8.30e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGCCACCGGCC  >  KT2440/4350232‑4350499
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cgcgTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGGGCGCCCGGTTGATCTa                                                                                                                                     >  8:259046/1‑137 (MQ=255)
   gTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCt                                                                                                                                  <  1:91158/137‑1 (MQ=255)
     aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGCCCCGTCGGCGCGGGCGCGGCCGGTTGATATACCTgg                                                                                                                                >  2:91158/1‑137 (MQ=255)
     aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTGATCTaacagg                                                                                                                                >  1:279937/1‑132 (MQ=255)
     aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTgg                                                                                                                                >  5:236023/1‑137 (MQ=255)
     aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCCTTTGATATACCTgg                                                                                                                                >  5:215195/1‑137 (MQ=255)
     aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCCCTGGCGCCACCCGTGCATCGACCTgg                                                                                                                                >  8:88825/1‑137 (MQ=255)
         tCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCTCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGc                                                                                                                            <  4:176426/137‑1 (MQ=255)
         tCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGc                                                                                                                            <  7:259046/137‑1 (MQ=255)
         gccATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGc                                                                                                                            <  3:131903/136‑1 (MQ=255)
           cATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGGGCGACCGGTTCATCGACCTGGCTGccc                                                                                                                          >  6:425158/1‑136 (MQ=255)
           cATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGGCCGGTTGATCTAACAGGaatccc                                                                                                                          >  4:190675/1‑132 (MQ=255)
           cATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGGGCGCCCCGTTAAGCTACCTGGATGccc                                                                                                                          >  2:63008/1‑136 (MQ=255)
              tGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTTGCGCGCCCGGTTCATCTACCTGGATGCCGATg                                                                                                                       >  6:69938/1‑137 (MQ=255)
                aTCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTTGATCTAACTGGATGGCGCTGGc                                                                                                                     >  4:447505/1‑137 (MQ=255)
                aTCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTTCATCTACCTGGATGCCGATGGc                                                                                                                     >  6:357428/1‑137 (MQ=255)
                aTCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGATTAATGTACCTGGATGCCCCTGGc                                                                                                                     >  6:457865/1‑137 (MQ=255)
                      gACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGGTGGCGCGGCCGGGTGATAGACCAGGGTGACGATGGCCAAccg                                                                                                               >  3:374393/1‑135 (MQ=255)
                      gACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTGATGGACCAGGATGCCGCTGGCCAAccg                                                                                                               >  7:15822/1‑135 (MQ=255)
                      gACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGGGCGCCCGGTTAATCGAACAGGATGCCCCTGGCCAAccg                                                                                                               >  7:76491/1‑135 (MQ=255)
                             tCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTTCATCTAACTGGCTGCCGAAGGCCCACCGGTAagcg                                                                                                        >  2:258512/1‑137 (MQ=255)
                              ggtggCCCCCCGCCAATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACCTTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTTGATGCCGATGGCCAACGGGTACGCGa                                                                                                       <  2:229568/134‑1 (MQ=255)
                               gAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTGATCTACCTGGCTGCCGATGGCCAACGGGGTCGCGAc                                                                                                      >  7:457371/1‑137 (MQ=255)
                                aGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACa                                                                                                     <  6:184789/137‑1 (MQ=255)
                                         gccCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGGCCCGTTCATCTACCAGGATGGCGCTGGCCAAACGGGACGCGACCCCGAAAccc                                                                                            >  8:123294/1‑137 (MQ=255)
                                         gccCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGGGCGGCCGGTTTTTCTTCCATGATGACGCTGGGCCACCGGTTAGCGCCCGCGAAAccc                                                                                            >  3:274434/1‑137 (MQ=255)
                                           ccATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCtt                                                                                          <  7:123294/137‑1 (MQ=255)
                                                cTTGACTGCCCCCTGCCGCGGAGCCTGCACTACGTTGTCGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCGAACGGGTACGCGACAGCGAAACCCTTgcgcg                                                                                     <  1:295599/137‑1 (MQ=255)
                                                  tGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCa                                                                                   <  8:20218/137‑1 (MQ=255)
                                                     cTGCCCCGTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATcg                                                                                <  1:283452/137‑1 (MQ=255)
                                                         ccccTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATcgccgc                                                                            <  4:436044/137‑1 (MQ=255)
                                                            cTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCAtt                                                                         <  6:236023/137‑1 (MQ=255)
                                                                   gcACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATc                                                                  <  6:138930/137‑1 (MQ=255)
                                                                        cTGCACTACTTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACTCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcccccc                                                             <  8:310210/137‑1 (MQ=255)
                                                                        cTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcccccc                                                             <  8:388757/137‑1 (MQ=255)
                                                                             cTACGTTGCCGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCt                                                        <  4:276076/137‑1 (MQ=255)
                                                                             cTACGTTGACGAGAGTCAGCCGGGCCTGACCGGTCGGCGCTGGGGCGACCGTTTCATCTACCTGGATGACGATGGCCAACGGGAACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCt                                                        <  8:1381/137‑1 (MQ=255)
                                                                             cTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCt                                                        <  1:178592/137‑1 (MQ=255)
                                                                             atACGTTGACGACAGTCAGCCGGGCCTGACGCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGTCAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCt                                                        <  7:6124/136‑1 (MQ=255)
                                                                              taggTTGACGACAGTCAGCCGGGCGTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACGTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTa                                                       <  1:396433/134‑1 (MQ=255)
                                                                              tACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTGATCTACCGGGATGCCCATGGCCCACGGGGAAGCGACAGCGCAACCCATGCGCGCCTCGCCGCATTGGTGCTCCCCCCCGCCTa                                                       >  7:117965/1‑137 (MQ=255)
                                                                                cGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTaca                                                     <  7:368328/137‑1 (MQ=255)
                                                                                cGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTaca                                                     <  1:52911/137‑1 (MQ=255)
                                                                                  ttGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGACCGGTTCATCTACCTGGATGCCGATGGCCCACCGGTACGCGCCAGCGAAACCCTTGCGCGCCTCGCCGCATTGGTGATCCCCCCGGCCTacccc                                                   >  7:227537/1‑134 (MQ=255)
                                                                                    gacgacAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTTGATCTACCGGGATGCCGAATGCCCACCGGGACGCGCCACCGCAACCCATGCGCGCCTCGCCGCATTGGTGGTCCCCCCCGCCTacaccgc                                                 >  6:425213/1‑134 (MQ=255)
                                                                                     acgacAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGAt                                                <  3:347992/137‑1 (MQ=255)
                                                                                        acAGTCAGCCGGGCCTGACCCGTCGGCGCGGGGGCGGCCGGTTGATCTTCCTGGATGGCGCTGGGCAACGGGGTAGCGGCAGCGAAACCCATGCGCGCATCGCCGCCTTTGTGATGCCCCCCGGCTACACGGCtgtg                                             >  4:117244/1‑137 (MQ=255)
                                                                                          aGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGAtgtgtg                                           <  3:447505/137‑1 (MQ=255)
                                                                                                         aCCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATcc                            <  7:463976/137‑1 (MQ=255)
                                                                                                                 gcgcTGGCGCGACCGGTTGATCTAACAGGATTCCGATGGCCAACGGGTACGCGCCAGCGAAACCCATGCGCGCAAAGCCGCAATGGTGATCCCCCCGGCCTACACGGATGTGTGGCTATTCGCCGCTCCGCAAGGcc                    >  8:218360/1‑137 (MQ=255)
                                                                                                                 gcgcGGGGGCGGCCGGTTGATCTACCAGGATGGCGCTGGCCCACGGGGTCGCGCCCGCGCAACCCATGCGCCCAACGCCCCATTTGTGGTCCCCCCCGCCAACACGGATGTGTGGATCTGCGCCGCTCCGCAAGGcc                    >  6:71557/1‑137 (MQ=255)
                                                                                                                 gcgcGGGCGCGCCCGGTTGATCTACCTGGATGCCGCTGGCCCACCGGTACGCGACCGCGAAACCCATGCGCGCATAGCCGCAATGGTGATACCCCCCGCCTACACCGATGTGTGGATCTGCGCCGATCCCCAACGcc                    >  8:463976/1‑137 (MQ=255)
                                                                                                                               gTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGCCAcc      >  8:130510/1‑137 (MQ=255)
                                                                                                                                   cATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGCCACCGGcc  <  5:42084/137‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                        
CGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGCCACCGGCC  >  KT2440/4350232‑4350499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑