| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 690,150 | T→G | intergenic (+27/‑2432) | bauC → / → PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 690,150 | 0 | T | G | 93.8% | 15.9 / ‑3.5 | 17 | intergenic (+27/‑2432) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (15/0); total (17/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-01 | |||||||||||
GGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑AGGCCGTCAGGCCTCCCCGC‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGC > KT2440/690028‑690250 | ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGCCGGGAGGGCGccccccg > 8:186714/1‑134 (MQ=255)ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGCCGGGACGCCGccccccc > 6:121307/1‑134 (MQ=255)ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGCCGGCAGGCCGccccccg > 2:64588/1‑134 (MQ=255) gcTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGGCGGCCGGGCGCCCCCC‑GGTG‑tttt > 8:278029/1‑137 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGG‑‑GGGCGGGCGGGCCACCCCCG‑GTTT‑TTTTTgggggg > 5:8971/1‑136 (MQ=255) cGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGGCGGCCGGCCGCCCCGC‑GGTG‑TTTTTTGGGGGTGggtgt > 3:432687/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCTGGGCCGAC‑GGCCGCCCCCC‑GGTG‑TTTTTGTGgggtggtgtt > 4:187154/1‑134 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGGCGGGCGGGCGCCCCCC‑GTTG‑TTTTTTGGgggtggggtgt > 7:275895/1‑132 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGGCGGGACGGCGCCCCGC‑GTTT‑TTTTTTTGGGGTGggtgtt > 6:236537/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGGCGGGACGCCCCCCCCC‑GTTGTTTTTTTGGggttgttttt > 1:102920/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGGCGGCCGGGCAACCCGCAGTTG‑TTTTTGTGggtgggtttt > 5:382078/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGGCGGCAGGGCGGCCCCC‑GGTG‑TGTTTTGGGGGTGggtgtt > 6:280215/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGCCGGGCGGCCGCCCCCC‑GTTG‑TTTTTTGGgggtggtgtgt > 2:292497/1‑133 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGC‑GGGCCGGCGGCCGCCCCCGC‑GGTG‑TTTTGGGGggtgggggttgg > 7:268702/1‑131 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGCCGGCAGGCCACCCCGC‑GTTG‑GTTTTTGGGGGTGgttgtttg > 6:445142/1‑135 (MQ=255) gCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑GGGGCGGCCGGCCCCCCCGC‑GGGG‑TTTTTTGGGGGTGGGTGTTGGGGTTGGGGGGGGACGGgtttttt > 2:161178/1‑134 (MQ=255) tAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑AGTCCGTCAGGCCTCCCCCC‑GTTG‑TTTTTGGGGGGTGGTTGTTGGTGTTGGGGAGGGACTGTTTTGAGAGGGTGTTTGCTGTGAGATGTTTACGCCACAACCCTTGc > 4:53317/1‑137 (MQ=255) | GGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑‑AGGCCGTCAGGCCTCCCCGC‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGC > KT2440/690028‑690250 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |