Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 1,998,653 G→A I48I (ATC→ATT)  CJ019_01900 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,998,6530GA97.2% 228.9 / ‑2.5 72I48I (ATC→ATT) CJ019_01900hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (37/33);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

TAAGCTTTCAATAGACTTATCATACATAAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATGATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGCGTACTACGTGCGAGGG  >  KT2440/1998521‑1998784
                                                                                                                                    |                                                                                                                                   
tAAGCTTTCAATAGACTTATCATACATAAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCAtaatga                                                                                                                                 >  8:136558/1‑137 (MQ=255)
     tttCAATAGACTTATCATACATAAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGa                                                                                                                            >  1:449470/1‑137 (MQ=255)
       tCAATAGACTTATCATACATAAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGatt                                                                                                                          <  8:53287/137‑1 (MQ=255)
       tCAATAGACTTATCATACATAAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGatt                                                                                                                          <  2:63805/137‑1 (MQ=255)
          aTAGACTTATCATACATAAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGattatt                                                                                                                       >  7:287535/1‑137 (MQ=255)
          aTAGACTTATCATACATAAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGattatt                                                                                                                       >  8:238005/1‑137 (MQ=255)
            aGACTTATCATACATAAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTAtttt                                                                                                                     >  3:60055/1‑136 (MQ=255)
                 tATCATACATAAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCtt                                                                                                                <  6:291926/137‑1 (MQ=255)
                 tATCATACATAAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCtt                                                                                                                <  4:309748/137‑1 (MQ=255)
                 tATCATACATAAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCCTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCTAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCtt                                                                                                                <  8:186741/137‑1 (MQ=255)
                    catacataAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGaa                                                                                                             >  5:127521/1‑137 (MQ=255)
                    catacataAGCTTGTCTATTTATGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTATCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAAAGGATTATTTGTTCTTGaa                                                                                                             >  2:94199/1‑137 (MQ=255)
                      tacataAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTTTCAGATGATAAGTGTTGCCTAATCTAAAGTCACCTAACATAATGAATGGATTATTTGTTCTTGAACa                                                                                                           >  3:102141/1‑137 (MQ=255)
                          taAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGctc                                                                                                       <  6:365187/137‑1 (MQ=255)
                           aaGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGctct                                                                                                      >  2:77753/1‑137 (MQ=255)
                            aGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCtt                                                                                                     <  5:141811/137‑1 (MQ=255)
                                tGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATg                                                                                                 >  5:91727/1‑137 (MQ=255)
                                tGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATg                                                                                                 <  6:17264/137‑1 (MQ=255)
                                tGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATg                                                                                                 >  4:379031/1‑137 (MQ=255)
                                 gTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCtt                                                                                                                <  5:238908/121‑1 (MQ=255)
                                 gTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCtt                                                                                                                >  6:238908/1‑121 (MQ=255)
                                  tCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGct                                                                                               <  6:127521/137‑1 (MQ=255)
                                   cTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCACATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGctc                                                                                              >  3:75359/1‑137 (MQ=255)
                                    tATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATGATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGctct                                                                                             >  5:446225/1‑137 (MQ=255)
                                    tATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGctct                                                                                             >  2:270263/1‑137 (MQ=255)
                                    tATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGctct                                                                                             >  4:226527/1‑137 (MQ=255)
                                    tATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGctct                                                                                             <  7:203904/137‑1 (MQ=255)
                                    tATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGctct                                                                                             >  5:457269/1‑137 (MQ=255)
                                    tATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGctct                                                                                             >  1:221622/1‑137 (MQ=255)
                                    tATTTCTGGGTTTATAAATTCTTTC‑TTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCtt                                                                                                                >  8:76030/1‑117 (MQ=255)
                                    tATTTCTGGGTTTATAAATTCTTTC‑TTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCtt                                                                                                                <  7:76030/117‑1 (MQ=255)
                                     aTTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCtt                                                                                                                <  6:392629/117‑1 (MQ=255)
                                     aTTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCtt                                                                                                                >  5:392629/1‑117 (MQ=255)
                                          tGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATCGAGTATTTGTTCTTGAACAGCTCTTGATGCACTCAAGcc                                                                                       >  6:438074/1‑137 (MQ=255)
                                           gggTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCa                                                                                      <  2:440047/137‑1 (MQ=255)
                                            ggTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCat                                                                                     >  6:454054/1‑137 (MQ=255)
                                               ttATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCatata                                                                                  >  2:209571/1‑136 (MQ=255)
                                                aataAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCg                                                                                 <  1:119487/136‑1 (MQ=255)
                                                         tttctttACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCAc                                                                        <  7:238005/137‑1 (MQ=255)
                                                           tctttACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTTCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCg                                                                      <  4:93727/137‑1 (MQ=255)
                                                           tctttACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCg                                                                      >  7:363585/1‑137 (MQ=255)
                                                                aCAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGAcccc                                                                 <  4:161892/137‑1 (MQ=255)
                                                                              gtTCTCATAATGTGTCAGATGATAAGTGTTCCCGAATCTATAGTCACCTACCAAAATGAATGGTTTTTTTGTTCTAGATCAGCTCATGATGCTCAGATGCCAAATAGGGACCCGCCCGACCCCAACAAACTCACAAt                                                   >  3:393909/1‑137 (MQ=255)
                                                                               ttCTCATTATGTGTCAGATGATAATTGTTTCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTATTGAACAGCTCTTGATTATCTCATGACATATCTGGAGCCCACCGACCACATGAAACTCACTAta                                                  >  8:89564/1‑136 (MQ=255)
                                                                                       aTGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCCACCCCATGAAACTCACTATCGATTTCaa                                          >  4:111195/1‑137 (MQ=255)
                                                                                         ttgtCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCaaaa                                        <  7:382268/136‑1 (MQ=255)
                                                                                         gtgtCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCaaaa                                        >  4:401173/1‑137 (MQ=255)
                                                                                             cAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCaaaaaaaa                                    >  5:88847/1‑136 (MQ=255)
                                                                                             cAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAAt                                    >  5:435204/1‑137 (MQ=255)
                                                                                             cAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAAt                                    >  5:307944/1‑137 (MQ=255)
                                                                                              aGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAAtc                                   <  1:77753/137‑1 (MQ=255)
                                                                                              aGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAAtc                                   <  4:212946/137‑1 (MQ=255)
                                                                                               gatgatAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCtt                                                                                                                <  5:374923/59‑1 (MQ=255)
                                                                                               gatgatAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCtt                                                                                                                >  6:374923/1‑59 (MQ=255)
                                                                                                aatatAAGTGTAGCCGAATCAAAAGTCACCTACCATAATGAATGGATAATTTGTTCTTGAACAGCACTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAAtctc                                 <  2:399046/134‑1 (MQ=255)
                                                                                                 tgatAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAAtctct                                >  6:201377/1‑137 (MQ=255)
                                                                                                 tgatAAGTGTTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAAtctct                                >  3:316060/1‑137 (MQ=255)
                                                                                                        tgtTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCtt                         <  3:379031/137‑1 (MQ=255)
                                                                                                        tgtTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCtt                         <  3:10255/137‑1 (MQ=255)
                                                                                                         gtTGCCGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCttt                        <  6:389348/137‑1 (MQ=255)
                                                                                                             ccGAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCa                    <  6:91727/137‑1 (MQ=255)
                                                                                                               gAATCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGc                  >  7:445019/1‑137 (MQ=255)
                                                                                                                aaTCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGCg                 >  7:248575/1‑137 (MQ=255)
                                                                                                                  tCTAAAGTCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGCGta               <  8:287535/137‑1 (MQ=255)
                                                                                                                         tCACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGCGTACTACGTg        <  6:457269/137‑1 (MQ=255)
                                                                                                                         ccACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGCGTACTACGTg        <  2:119580/136‑1 (MQ=255)
                                                                                                                          cACCTACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGCGTACTACGTGc       >  1:160161/1‑137 (MQ=255)
                                                                                                                            ccTACCATGATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGCGTACTACGTGCGa     <  6:446225/137‑1 (MQ=255)
                                                                                                                              tACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGCGTACTACGTGCGAgg   <  5:454054/137‑1 (MQ=255)
                                                                                                                              tACCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCGCAGCCTTTTCCAGCGTACTACGTGCGAgg   >  4:139324/1‑137 (MQ=255)
                                                                                                                              aaCCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGCGTACTACGTGCGAgg   <  3:5886/136‑1 (MQ=255)
                                                                                                                              aaCCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGCGTACTACGTGCGAgg   <  6:338079/136‑1 (MQ=255)
                                                                                                                               aCCATAATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGCGTACTACGTGCGAggg  >  4:264561/1‑137 (MQ=255)
                                                                                                                                    |                                                                                                                                   
TAAGCTTTCAATAGACTTATCATACATAAGCTTGTCTATTTCTGGGTTTATAAATTCTTTCTTTACAATGAAGGCGTCGTTCTCATTATGTGTCAGATGATAAGTGTTGCCGAATCTAAAGTCACCTACCATGATGAATGGATTATTTGTTCTTGAACAGCTCTTGATGCTCTCATGCCATATCGGGAGCCCACCGACCCCATGAAACTCACTATCGATTTCAAAAAAATCTCTCAGCCTTTTCCAGCGTACTACGTGCGAGGG  >  KT2440/1998521‑1998784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑