Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 3,061,793 G→A S592N (AGC→AAC)  qedA_2 → Quinoprotein alcohol dehydrogenase (cytochromec)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,061,7930GA95.8% 159.1 / NA 48S592N (AGC→AAC) qedA_2Quinoprotein alcohol dehydrogenase (cytochromec)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (26/20);  minor base C (0/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGC  >  KT2440/3061658‑3061926
                                                                                                                                       |                                                                                                                                     
aCGTTTCGGTAGTCTCCGGCT‑GGGCGGCGAGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTTGACCTTCAAGTTGCCAAAGCAGTTGCAGCATACGGCAAACg                                                                                                                                     <  2:22422/137‑1 (MQ=255)
 ggTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCGGTGGGGCGGCGAAGTGGCCAAACGGGTCAATGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                     <  6:67516/136‑1 (MQ=255)
 cGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                     <  1:313944/137‑1 (MQ=255)
 cGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                     <  1:133073/137‑1 (MQ=255)
 cGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                     <  4:172937/137‑1 (MQ=255)
 cGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                     <  3:96876/137‑1 (MQ=255)
       ggTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGc                                                                                                                               <  1:273101/137‑1 (MQ=255)
       ggTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGc                                                                                                                               >  4:325276/1‑137 (MQ=255)
         tAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAATCAGTTGCAGCAAACGGCAAACGTCAAGCCa                                                                                                                             <  8:371044/137‑1 (MQ=255)
         tAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCa                                                                                                                             >  4:403924/1‑137 (MQ=255)
          aGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCAt                                                                                                                            <  5:427893/137‑1 (MQ=255)
            tctcCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa                                                                                                                          <  1:175184/137‑1 (MQ=255)
              gccGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATATCg                                                                                                                        <  4:74262/136‑1 (MQ=255)
              gccGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACg                                                                                                                        <  5:458483/136‑1 (MQ=255)
                 ggCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTAAACCAGGGCGGCATGCTCTGGACCTTCAATTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACgcct                                                                                                                     >  1:167721/1‑137 (MQ=255)
                           gcgcGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGcc                                                                                                           <  4:281140/137‑1 (MQ=255)
                               ggTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCtt                                                                                                       >  1:95205/1‑137 (MQ=255)
                                gTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTtg                                                                                                      >  3:265630/1‑137 (MQ=255)
                                   ccGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTtgctg                                                                                                   <  4:222549/137‑1 (MQ=255)
                                        gtgGGTCGGCGAAGTGGCCAAACTGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTTCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCt                                                                                              <  3:315691/137‑1 (MQ=255)
                                        gtgGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCt                                                                                              <  3:403924/137‑1 (MQ=255)
                                            ggcggcGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATc                                                                                          <  8:306146/137‑1 (MQ=255)
                                              cggcgAAGTGGCCAAACGGGTCAAGGACTTCACCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCCAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCgg                                                                                        >  5:136735/1‑137 (MQ=255)
                                              cggcgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTGAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCgg                                                                                        >  2:41406/1‑137 (MQ=255)
                                              cggcgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCgg                                                                                        >  8:55652/1‑137 (MQ=255)
                                                           aaaCGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGccc                                                                           >  1:172451/1‑137 (MQ=255)
                                                            aaCGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCct                                                                          <  1:272727/137‑1 (MQ=255)
                                                              cGGGTCAAGGACTTCAACCCGGGGGGCATGCTCTGTCCCTTCACGTGCCCCACGCAGTTGCAGCAAACGGAAACCGTCACGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAGCGGGGGCAAGCGGCCctac                                                                        <  6:430315/137‑1 (MQ=255)
                                                                 ctCAAGCACTGCAACCAGGGCGGCATGCGCTGCCCCTTCAAGTGCCCCAAGCTGTTGCACAAAACGACAACGGTCACGCAATAACGCCTGCCTACTGCGGGTTGCTGCCGGTTATCGGTGGAAAGCGGCCCtactac                                                                     <  4:13813/136‑1 (MQ=255)
                                                                      ggACTTCAACCAGGGCGGCATGCGCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGCAAAACGTCAAGCCATAACGCCTGCGTACTGCCGCTTGCTGCCGCTCATGGGTGGCAAGCGGCCCTACTACCAAAt                                                                <  2:273347/137‑1 (MQ=255)
                                                                         cTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCAAACCGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGGGGCAAGCGGCCCTACTACCAAATGAc                                                             <  3:325276/137‑1 (MQ=255)
                                                                              aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGagag                                                        >  8:135731/1‑137 (MQ=255)
                                                                               ccAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCACGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGc                                                       >  7:384350/1‑137 (MQ=255)
                                                                                     cggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCt                                                 >  7:243831/1‑137 (MQ=255)
                                                                                      ggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGAGGCAAGCGGCCATACTACCAAATGACGACAGCCCCGCTg                                                >  7:39530/1‑137 (MQ=255)
                                                                                             tctGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGa                                         >  6:243352/1‑137 (MQ=255)
                                                                                              ctGTACCTACAAGTTGCCCAAGCATTTCCAGCAAACTGCAAACGTCAAGCCATAACCCCGGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGAt                                        <  5:69005/137‑1 (MQ=255)
                                                                                                ggACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGc                                      >  4:379830/1‑137 (MQ=255)
                                                                                                ggACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACAACCAAATGACGAGAGCCCCGCTGCCAACCATGc                                      >  8:181382/1‑137 (MQ=255)
                                                                                                   ccTACAAGTTCCCCAAGCAGTTGCAGAAACCGGCAAACGTCAAGCCATAACCCCTGCCTACTCCCGCTCGCTGCCGCTGAGCGGTGGCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCAtt                                   <  7:86793/137‑1 (MQ=255)
                                                                                                         aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAATCCATAACGCCTGCCTACTTCAGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACCAGATCCACGCTGCCAACGATGCAATCGcccc                             >  5:71372/1‑136 (MQ=255)
                                                                                                         aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGcccc                             >  3:65390/1‑136 (MQ=255)
                                                                                                         aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGcccc                             >  1:43624/1‑136 (MQ=255)
                                                                                                         aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGcccc                             >  3:16875/1‑136 (MQ=255)
                                                                                                         aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCg                             >  4:39260/1‑137 (MQ=255)
                                                                                                         aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCg                             >  5:236759/1‑137 (MQ=255)
                                                                                                          gTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCgg                            >  7:21822/1‑137 (MQ=255)
                                                                                                          gTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCgg                            >  8:78897/1‑137 (MQ=255)
                                                                                                                aaaGCAGTTCCAGCAAACGGCAAACGTCAAGCAATAACGCCTCCCTACTGCCGCTTGCTGCCGCTTATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcgcg                      <  8:243831/136‑1 (MQ=255)
                                                                                                                            gcaaacggcaaacgTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAcc          >  7:91588/1‑137 (MQ=255)
                                                                                                                            gcaaacggcaaacgTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAcc          >  1:372246/1‑137 (MQ=255)
                                                                                                                            ccaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAcc          <  6:138198/136‑1 (MQ=255)
                                                                                                                                 cgccAAACGTCAACCCATACCGCCTCCCTACTGCCGGTTGCGGCCGCTTAGGGGGGCCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCgg     <  5:42861/134‑1 (MQ=255)
                                                                                                                                  ggCAAACGTCAAGCCATAACGCCTACCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGt    >  5:423616/1‑137 (MQ=255)
                                                                                                                                    cAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGc  >  3:182961/1‑137 (MQ=255)
                                                                                                                                       |                                                                                                                                     
ACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGC  >  KT2440/3061658‑3061926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑