Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	4,223,606	G→A	L83L (CTG→TTG)	CJ019_03962 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,223,606	0	G	A	95.4%	207.3 / 0.0	65	L83L (CTG→TTG)	CJ019_03962	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (31/31);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

GACCAGATATCTTCTCAATCAATTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAGCCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATAAATGATCTGAGCTCAGACCAATCGTCGCCATCAATAAAA  >  KT2440/4223474‑4223739                                                                                                                                     |                                                                                                                                      gACCAGATATCTTCTCAATCAATTCGCACATGCCGACTTGTTCGTACTGGCTAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGc                                                                                                                                   <  8:113381/137‑1 (MQ=255) gACCAGATATCTTCTCAATCAATTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGc                                                                                                                                   <  2:154289/137‑1 (MQ=255)     aGATATCTTCTCAATCAATTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGc                                                                                                                               >  2:280557/1‑137 (MQ=255)          tcttctCAATCAATTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAGCCGCAAGCTTGGc                                                                                                                          >  7:441248/1‑137 (MQ=255)          tcttctCAATCAATTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGc                                                                                                                          >  3:287426/1‑137 (MQ=255)           cttctCAATCAATTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCa                                                                                                                         <  5:127096/137‑1 (MQ=255)           cttctCAATCAATTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCa                                                                                                                         <  4:287426/137‑1 (MQ=255)           attctCAATCAATTCTGAAATTCCGTGTTGTTCGTACTGTCTAATCAGTGAGCTCGCGACATCCTGGAAGAACTTATATGCTTTAAATTTTTTGCTCGACGTATCGTAAGAGATGGGATGCAACCGCAAGCTTGGCa                                                                                                                         <  3:439506/136‑1 (MQ=255)             tctcAATCAATTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGa                                                                                                                       <  3:374798/137‑1 (MQ=255)              ctcAATCAATTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGaa                                                                                                                      >  1:171278/1‑137 (MQ=255)                caatcaatTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAAcc                                                                                                                    <  1:365325/137‑1 (MQ=255)                    caatTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGTCAGAACctctc                                                                                                                >  3:387433/1‑137 (MQ=255)                    caatTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACctctc                                                                                                                >  5:363823/1‑137 (MQ=255)                    caatTCGCAAATGCCGACTTGTTCGTACTGGCAAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGGACGAGATGGGATGCAACCGCAAGCTTGGCAGAACctctc                                                                                                                >  1:179530/1‑137 (MQ=255)                     aatTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACctctct                                                                                                               <  2:456387/137‑1 (MQ=255)                              aTGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCTACCGCAAGCTTGGCAGAACCTCTCTCGGTAGtta                                                                                                      >  2:173773/1‑136 (MQ=255)                                          tCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTccc                                                                                          <  4:323493/137‑1 (MQ=255)                                                 ggCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATg                                                                                   >  3:118277/1‑137 (MQ=255)                                                  gCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGt                                                                                  <  7:183658/137‑1 (MQ=255)                                                   cGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTc                                                                                 <  5:341725/137‑1 (MQ=255)                                                   cGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTc                                                                                 <  7:382048/137‑1 (MQ=255)                                                     aaTCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAGCCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCaa                                                                               >  2:449048/1‑137 (MQ=255)                                                     aaTCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCaa                                                                               <  3:94444/137‑1 (MQ=255)                                                     aaTCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAAACGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTACCATCGATGTCaa                                                                               <  6:144278/137‑1 (MQ=255)                                                     aaTCAGAGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCaa                                                                               <  2:151707/137‑1 (MQ=255)                                                       tCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAAt                                                                             <  6:363823/137‑1 (MQ=255)                                                          gTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACg                                                                          >  2:341541/1‑137 (MQ=255)                                                               cgagcgACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTAAGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTtat                                                                     <  8:154803/134‑1 (MQ=255)                                                               cTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTTCGAGATGGGATGCAACCGCAATCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTtat                                                                     <  1:173773/137‑1 (MQ=255)                                                                               cGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAgactga                                                     >  6:84170/1‑137 (MQ=255)                                                                                 aaCTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAgactgact                                                   <  3:279879/137‑1 (MQ=255)                                                                                   cTTATAAGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGAATGACTca                                                 >  1:207692/1‑137 (MQ=255)                                                                                    ttATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAGCCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTcac                                                <  1:449048/137‑1 (MQ=255)                                                                                    ttATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTcac                                                <  4:205765/137‑1 (MQ=255)                                                                                    ttATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTcac                                                <  7:356848/137‑1 (MQ=255)                                                                                    ttATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGGTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTcac                                                <  5:349050/137‑1 (MQ=255)                                                                                     tataTGCTTTAAATTTTTTGCTAGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCTATGTCAAATACGCTTATATAATCTTCAGACTGACTcaca                                               >  7:378106/1‑136 (MQ=255)                                                                                      atatGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTcacca                                              >  2:273536/1‑137 (MQ=255)                                                                                      atatGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTcacca                                              >  6:87762/1‑137 (MQ=255)                                                                                      atatGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTcacca                                              >  2:302814/1‑137 (MQ=255)                                                                                         tGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCAccacca                                           <  1:280557/137‑1 (MQ=255)                                                                                           cTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATa                                         <  5:141992/137‑1 (MQ=255)                                                                                               aaaTTTTTTGCTCGACCTATCGTACGAGATGTGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGATCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGCATCACCACCATAAATg                                     >  4:375067/1‑137 (MQ=255)                                                                                               aaaTTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGtt                                                                                             <  5:445448/81‑1 (MQ=255)                                                                                               aaaTTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGtt                                                                                             >  6:445448/1‑81 (MQ=255)                                                                                               aaaTTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCa                                                           >  6:61070/1‑115 (MQ=255)                                                                                               aaaTTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCa                                                           <  5:61070/115‑1 (MQ=255)                                                                                               aaaTTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATAAAtt                                     >  7:224519/1‑136 (MQ=255)                                                                                                 aTTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATc                                                                                       >  2:135195/1‑85 (MQ=255)                                                                                                 aTTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATc                                                                                       <  1:135195/85‑1 (MQ=255)                                                                                                 aTTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATAAATGAt                                   >  1:275673/1‑137 (MQ=255)                                                                                                 aTTTTTTGCTCGACCTATCGTACGAGATGGGATGCAACCGCAAGCTTGGAAGAACCTATCTAGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATAAATGAt                                   >  2:263783/1‑137 (MQ=255)                                                                                                                    gTACGAGATGGGATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATAAATGATCTGAGCTCAGACCAAtcgt                >  8:193316/1‑137 (MQ=255)                                                                                                                              ggATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATAAATGATCTGAGCTCAGACCAATCGTCGCCATCaat      >  6:101149/1‑137 (MQ=255)                                                                                                                              ggATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACAATAAATGATCTGAGCTCAGACAAATCGTCGCCATCaat      >  2:116228/1‑137 (MQ=255)                                                                                                                               gATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTc                                                                                 >  8:106774/1‑61 (MQ=255)                                                                                                                               gATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTc                                                                                 <  3:123989/61‑1 (MQ=255)                                                                                                                               gATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTc                                                                                 <  7:106774/61‑1 (MQ=255)                                                                                                                               gATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTc                                                                                 >  4:123989/1‑61 (MQ=255)                                                                                                                               gATGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATAAATGATCTGAGCTCAGACCAATCGTCGCCATCaata     >  5:46607/1‑137 (MQ=255)                                                                                                                                aTGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATAAATGATCTGAGCTCAGACCAATCGTCGCCATCaataa    >  2:113249/1‑137 (MQ=255)                                                                                                                                aTGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATAAATGATCTGAGCTCAGACCAATCGTCGCCATCaataa    <  3:269547/137‑1 (MQ=255)                                                                                                                                aTGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATAAATGATCTGAGCTCAGACCAATCGTCGCCATCaataa    >  2:383075/1‑137 (MQ=255)                                                                                                                                 tGCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATAAATGATCTGAGCTCAGACCAATCGTCGCCATCaataaa   <  3:229132/137‑1 (MQ=255)                                                                                                                                  gCAACCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCATATACGCTTATATAATCTTCAGACTGACTCACCACCATAAATGATCTGAGCTCAGACCAATCGTCGCCATCaatacaa  >  7:191631/1‑137 (MQ=255)                                                                                                                                     |                                                                                                                                      GACCAGATATCTTCTCAATCAATTCGCAAATGCCGACTTGTTCGTACTGGCGAATCAGTGAGCTCGCGACATCCTGGACGAACTTATATGCTTTAAATTTTTTGCTCGACCTATCGTACGAGATGGGATGCAGCCGCAAGCTTGGCAGAACCTCTCTCGGTAGTTCCTGAGCGTTCCCATCGATGTCAAATACGCTTATATAATCTTCAGACTGACTCACCACCATAAATGATCTGAGCTCAGACCAATCGTCGCCATCAATAAAA  >  KT2440/4223474‑4223739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑