Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,331,148 Δ1 bp 7.1% intergenic (‑30/‑117) cynR_1 ← / → ybhH HTH‑type transcriptional regulator CynR/Putative isomerase YbhH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,331,1480T. 7.1% 188.9 / 7.2 56intergenic (‑30/‑117)cynR_1/ybhHHTH‑type transcriptional regulator CynR/Putative isomerase YbhH
Reads supporting (aligned to +/- strand):  ref base T (14/38);  new base . (2/2);  total (16/40)
Fisher's exact test for biased strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCAGGGCCGGCTGGGACAAGTGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTC  >  KT2440/2331014‑2331277
                                                                                                                                      |                                                                                                                                 
gCTCAGGGCCGGCTGGGACAAGTGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTAt                                                                                                                                   >  1:405468/1‑135 (MQ=255)
        ccGGCTGGGACAAGTGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTg                                                                                                                          <  7:304137/136‑1 (MQ=255)
         cGGCTGGGACAAGTGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTGCATAAGAAACCTTCGATTGGACTGGTACTTTATTCATGTTTg                                                                                                                          <  2:3695/135‑1 (MQ=255)
         cGGCTGGGACAAGTGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTg                                                                                                                          <  3:410630/135‑1 (MQ=255)
         cGGCTGGGACAAGTGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTg                                                                                                                          <  3:161570/135‑1 (MQ=255)
         cGGCTGGGACAAGTGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTg                                                                                                                          <  2:104374/135‑1 (MQ=255)
              gggACAAGTGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATc                                                                                                                      >  6:442981/1‑134 (MQ=255)
              gggACAAGTGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATc                                                                                                                      >  2:355377/1‑134 (MQ=255)
              gggACAAGTGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTACATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATc                                                                                                                      >  6:312059/1‑134 (MQ=255)
                    aGTGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCaataat                                                                                                              <  7:150632/136‑1 (MQ=255)
                      tGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAg                                                                                                            <  8:244942/136‑1 (MQ=255)
                        gAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCt                                                                                                          <  8:141186/136‑1 (MQ=255)
                         aGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCt                                                                                                          >  7:168871/1‑135 (MQ=255)
                                cggcggCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGatat                                                                                                   >  3:169601/1‑135 (MQ=255)
                                 ggcggcCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATAttt                                                                                                 <  8:168871/136‑1 (MQ=255)
                                 ggcggcCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATAttt                                                                                                 <  7:354174/136‑1 (MQ=255)
                                  gcggcCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATAttt                                                                                                 <  1:355377/135‑1 (MQ=255)
                                  gcggcCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATAttt                                                                                                 <  6:325241/135‑1 (MQ=255)
                                  gcggcCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATAttt                                                                                                 <  3:312245/135‑1 (MQ=255)
                                       cTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCAtt                                                                                            <  3:316708/135‑1 (MQ=255)
                                       cTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCAtt                                                                                            <  6:81647/135‑1 (MQ=255)
                                       cTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCAtt                                                                                            <  3:95755/135‑1 (MQ=255)
                                           tGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGgat                                                                                        <  4:203259/135‑1 (MQ=255)
                                            ggAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGgatt                                                                                       <  5:137982/135‑1 (MQ=255)
                                            ggAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGgatt                                                                                       <  4:134581/135‑1 (MQ=255)
                                                       aGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTatccatc                                                                           >  7:153101/1‑136 (MQ=255)
                                                       aGTTCGGCGATTTTCAAGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTatccat                                                                            <  4:193708/135‑1 (MQ=255)
                                                          tCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGGCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATccc                                                                         <  4:118108/135‑1 (MQ=255)
                                                          tCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATccc                                                                         <  5:369301/135‑1 (MQ=255)
                                                          tCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATccc                                                                         <  2:42773/135‑1 (MQ=255)
                                                            ggCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTg                                                                       <  8:346108/135‑1 (MQ=255)
                                                             gCGATTTTCATGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTg                                                                       <  1:308026/134‑1 (MQ=255)
                                                             gCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTg                                                                       <  5:145831/134‑1 (MQ=255)
                                                                      aCGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTg                                                            >  7:107360/1‑136 (MQ=255)
                                                                        gAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGc                                                           <  1:197022/135‑1 (MQ=255)
                                                                        gAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGc                                                           <  2:193895/135‑1 (MQ=255)
                                                                        gAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGc                                                           <  5:221423/135‑1 (MQ=255)
                                                                        gAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCt                                                          <  7:363384/136‑1 (MQ=255)
                                                                                          cAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTA‑TCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCt                                                          <  1:334142/117‑1 (MQ=255)
                                                                                          cAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTA‑TCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCt                                                          >  2:328230/1‑117 (MQ=255)
                                                                                          cAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTA‑TCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCt                                                          >  2:334142/1‑117 (MQ=255)
                                                                                          cAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTA‑TCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCt                                                          <  1:328230/117‑1 (MQ=255)
                                                                                           aGCTCATATTCCATAAGAAACCTTCGATTTGCCTGGTACTTTATTCATGTTTGAATCGCATTAATATCTTCGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTAACAGAATCAATGATGTc                                        <  3:260432/135‑1 (MQ=255)
                                                                                           aGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTAc                                                                                                        <  6:16396/71‑1 (MQ=255)
                                                                                           aGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTAc                                                                                                        >  5:16396/1‑71 (MQ=255)
                                                                                           aGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTc                                        <  3:380819/135‑1 (MQ=255)
                                                                                             cTCATATTCCATAAGAAACCTTCGATTTGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCa                                      <  6:198162/135‑1 (MQ=255)
                                                                                             cTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGACATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCa                                      >  4:237058/1‑135 (MQ=255)
                                                                                                      cATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATcaa                              <  5:442981/134‑1 (MQ=255)
                                                                                                      cATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATcaa                              <  5:312059/134‑1 (MQ=255)
                                                                                                          aGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAaga                         >  5:107718/1‑135 (MQ=255)
                                                                                                                 ttCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGaaa                 >  7:419859/1‑136 (MQ=255)
                                                                                                                   cGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAAt                >  8:78871/1‑135 (MQ=255)
                                                                                                                    gATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAAt                <  5:144799/134‑1 (MQ=255)
                                                                                                                      ttGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATcaa                              <  7:291133/118‑1 (MQ=255)
                                                                                                                      ttGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATcaa                              >  8:291133/1‑118 (MQ=255)
                                                                                                                                 cTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTc  >  1:124964/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                 
GCTCAGGGCCGGCTGGGACAAGTGGAGCGCTTCGGCGGCCTCATGGAAACTGCCCAGTTCGGCGATTTTCACGAAAGATCTGATGTCTTGCAGCTCATATTCCATAAGAAACCTTCGATTGGCCTGGTACTTTATTCATGTTTGAATCGCAATAATAGCTACGATATTTGCATTGGATTGATTTATCCATCCCTGTCACTCTTTTGCTCACAGCATCAATGATGTCCATTGATCAACAAGAGTGCGAAATCATGCAACGAATTC  >  KT2440/2331014‑2331277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑