Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 275,958 A→C 23.1% T121P (ACC→CCC)  betT2 → Osmo‑dependent choline transporter BetT2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440275,9580AC23.1% 46.5 / 2.2 39T121P (ACC→CCC) betT2Osmo‑dependent choline transporter BetT2
Reads supporting (aligned to +/- strand):  ref base A (10/19);  new base C (4/6);  total (14/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACGGCAAGATCAAACTCGGTGCCGACCACGACGAACCCGAGTTCAGCTACCTGTCGTGGGCCGGCATGCTGTTCGCCGCCGGCATCAGCATCACGTTGTTCTTCTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCTGGGGAGTGTTCGCCTTCGTCGGCATGGCATTGGCCTATT  >  KT2440/275832‑276093
                                                                                                                              |                                                                                                                                       
tACGGCAAGATCAAACTCGGTGCCGACCACGACGAACCCGAGTTCAGCTACCTGTCGTGGGCCGGCATGCTGTTCGCCGCCGGCATCAGCATCACGTTGTTCTTCTTCTGTGTCTCCGAACCGCTGACCCACAagc                                                                                                                                >  7:99474/1‑136 (MQ=255)
         aTCAAACTCGGTGCCGACCACGACGAACCCGAGTTCAGCTACCTGTCGTGGGCCGGCATGCTGTTCGCCGCCGGCATCAGCATCACGTTGTTCTTCTTCTGTGTCTCCGAACCGCTGAGCCACATGCTGCAAccgc                                                                                                                       <  8:42369/136‑1 (MQ=255)
           cAAACTCGGTGCCGACCACGACGAACCCGAGTTCAGCTACCTGTCGTGGGCCGGCCTGCAGTTAGCCGCCGGCATCAGCCTAACCTTGTTGTGCGTCTGTGTCTCCGCACCGCGGCCCCACCTGCCGCCAccgcc                                                                                                                      >  5:174575/1‑135 (MQ=255)
           cAAACTCGGTGCCGACCACGACGAACCCGAGTTCAGCTACCTGTCGTGGGCCGGCATGCTGTTCGCCGCCGGCATCAGCATCACGTTGTTCTTCTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAAccgcc                                                                                                                      >  4:166679/1‑135 (MQ=255)
                     gCCGACCACGACGAACCCGAGTTCAGCTACCTGTCGTGGGCCGGCATGCTGTTCGACGCCGGCATCAGCAAGCCGTTGTTCTTCTTCTGTTTCTCCGAACCGCTGCACCACATGCTGCAAACGCCGCAAGGCGCAg                                                                                                           >  8:103702/1‑136 (MQ=255)
                         aCCACGACGAACCCGAGTTCAGCTCCCTGGCGGGGGCCGGCAGGCTGTGCGCCGCCGGCATCAGCATCACGTTGTTCTTCTTCTGTGTCGCCGACCCGCTGACCCACATGCTCCACCCGCCGCAAGGCGAAGCCg                                                                                                        <  4:374224/135‑1 (MQ=255)
                         aCCACGACGAACCCGAGTGCAGCTCCCTGTCGTGGGCCGGCATGCTGTCCGCCGCCGGCATCAGCATCACGTTGTTCTTCTTCTGGGTCTCCGAACCGCTCACCCACATGCTGCAACCGCCGCAAGGCGAAGCCg                                                                                                        <  4:60230/135‑1 (MQ=255)
                          ccACGACGAACCCGAGTTCAGCTACCTGTCGTGGGCCGGCCTGCTGTTAGCCGCCGGCCTCCGCCTCCCGGTGTTGTTGTTGTGTGTGTCCGAACCGCGGCCCCACCAGCTGCCACCCCCCCCACGCGCCGCCgg                                                                                                       >  6:241304/1‑135 (MQ=255)
                            acgacgAACCCGAGTTCAGCTACCTGTCGTGGGCCGGCATGCTGTTAGCCGCCGGCATCAGCATCACGGTGTTCTTCTTATGTGTCTGCGAACCGCTGACCCACAAGCTGCCACCGCCGCAAGGCCAAGCCGGCAc                                                                                                    >  7:9413/1‑136 (MQ=255)
                                   aCCCGAGTTCAGCTCCCTGTCGTGGGCCGGCATGCTGTGCGCCGCCGGCATCCGCATCACGTTGTTCTTCTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAg                                                                                             <  8:368612/136‑1 (MQ=255)
                                   aCCCGAGTTCAGCTCCCTGGCGGGGGCCGCCAGGCTGCGCGCCGCCGGCAGCTCCATCACGTTGTTATGCTGCGGTGGCGCCGAACCGCGGACCCCCATCCTCCAACCGCCGCAAGGCGAAGCCGGCACGGTCGa                                                                                              <  6:148424/135‑1 (MQ=255)
                                         gTTCAGCTACCGGTCGGGGGCCGGAATGCTGTGCGCCGCCGGCATCAGCATCACGTTGTGCTGCTTCTGTGGCTCCGACCCGCTGCCCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCgg                                                                                        <  6:234162/135‑1 (MQ=255)
                                                  ccTGTCGTGGGCCGGCATGCTGTTCGCCGCCGGCATCAGCATCACGTTGTTGTTCTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAAACCCCGCAAGGCGAACCCGGCACGGTCGAGGCCGGGCGCCAGGc                                                                               >  6:101419/1‑135 (MQ=255)
                                                         tGGGCCGGCAGGCTGTGCGCCGCCGGCATCAGCAGCACGTTGTTCTTCTTCTGTGTCTCCCACCCGCTGACCCACATGCTGCACCCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGa                                                                       <  8:261889/136‑1 (MQ=255)
                                                          gggCCGGCATGCTGTTCGCCGCCGGCATCAGCATCCCGTTGTTGTTCTTCTGTGTCTCCGAACCGCTGCCCCACATGCTGCAACCCCCGCAAGGCGAAGCCGGCACGGTCGAGGCCCGGCGCCCGGCGCTGCAGa                                                                       >  3:349571/1‑135 (MQ=255)
                                                          gggCCGGCATGCTGTGCGCCGCCGCAATCAGCATCACGTTGTTCTTCTTCGGTGCCACCCAACCGCTCACCCACATGCTGCACCCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGa                                                                       <  4:39529/135‑1 (MQ=255)
                                                                     cTGTTCGCCGCCGTCATCATCATCACGTTGTTCTTCTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGc                                                           <  8:331266/136‑1 (MQ=255)
                                                                      tgtgCGCCGCCGGCATCAGCATCACGTTGTTCTTCTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGc                                                           <  4:318696/135‑1 (MQ=255)
                                                                        ttggccgcgGGAATAAGCATCACTTTGTTCTTGTTCTGTGTCTCCGAACCGCTTACCCACATGCTGCAACCGCCGAAAGGGGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACt                                                        <  8:344129/133‑1 (MQ=255)
                                                                         gcgccgcCGGCAGCCGCAGCCCGGGGGTCTTCTGCGGTGCCGCCCACCCGCGCCCCCACAGGCCCCCCCCCCCCCACGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACt                                                        <  1:375808/134‑1 (MQ=255)
                                                                           gccgccGGCATCAGCATCACGTTGTTCTTGTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTgg                                                      <  2:287331/135‑1 (MQ=255)
                                                                             cgccgGCATCAGCATCACGTTGTTCTTCTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTgggg                                                    >  1:217321/1‑135 (MQ=255)
                                                                             cgccgGCATCAGCATCACGTTGTTCTTCTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGc                                                   >  8:70199/1‑136 (MQ=255)
                                                                             cgccgGCATCAGCATCACGTTGGTCTTCTTGTGGGTCTCCGACCCGCTGACCCACATGCTGCACCCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGc                                                   <  8:72298/136‑1 (MQ=255)
                                                                              cccgCCATCAGCATCACGTGGTTCTTCTTCTGTGGCCCCCCCCCGCTGCCCCACAGGCGCCACCCCCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGc                                                   <  5:214407/134‑1 (MQ=255)
                                                                                cgGCATCAGCATCACGTTGTTCTTCTTCTGGGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCt                                                 <  6:232397/135‑1 (MQ=255)
                                                                                cgGCAGCAGCTACCCGGTGTTGTTGTGGTGTGTCCCCGACCCGCGCCCCCACAGCCTCCAACCGCCGCACGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCt                                                 <  5:331286/135‑1 (MQ=255)
                                                                                   catcagcatcaCGTTGTTCTTCTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGa                                                                       >  8:80120/1‑110 (MQ=255)
                                                                                   catcagcatcaCGTTGTTCTTCTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGa                                                                       <  7:80120/110‑1 (MQ=255)
                                                                                        gaatcaCGTTGTTCTTGTTCTGTGTATCCCAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCt                                         <  4:336517/133‑1 (MQ=255)
                                                                                                ttgttCTTCTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCTGGGGAgtg                                 >  6:348481/1‑135 (MQ=255)
                                                                                                    tcttcttctGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCAGCACTGGGGCCTGCATGGCTGGGGAGTGTTCg                             >  5:409048/1‑135 (MQ=255)
                                                                                                    tcttcttctGTGGCTCCGACCCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCTGGGGAGTGTTc                              <  3:205059/134‑1 (MQ=255)
                                                                                                      ttcttctGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCTGGGGAGTGTTCGcc                           >  5:191269/1‑135 (MQ=255)
                                                                                                             gtgtCTCCGACCCGCTGACCCACATGCTGCCACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCTGGGGAGTGTTCGCCTtcgtcg                    <  2:265277/135‑1 (MQ=255)
                                                                                                             gtgtCCCCGAACCCCGGCCCCACATGCTGACCCCCCCCCAGGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCTGGGGAGTGTTCGCCTtcgtcg                    <  5:155633/135‑1 (MQ=255)
                                                                                                              tgtCTCCGAACCGGCCCCCCACAGCCTGCACCCGCCGCAAGGTGAAGCCGGCACGGGCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCTGGGGAGTGTTCGCCTtcgtcg                    <  7:332595/134‑1 (MQ=255)
                                                                                                                 ctcCGAACGGGTGACCCACATCCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCTGGGGAGTGTTCGCCTTCGTCggcatg               <  8:155569/136‑1 (MQ=255)
                                                                                                                  gccGAACCGCTGACCCACTTGCTGCACCCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCTGGGGAGTGTTCGCCTTCGTCggcatg               <  6:191269/134‑1 (MQ=255)
                                                                                                                              aCCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCTGGGGAGTGTTCGCCTTCGTCGGCATGGCATTGGCCTAtt  >  7:295908/1‑136 (MQ=255)
                                                                                                                              aCCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCTGGGGAGTGTTCGCCTTCGTCGGCATGGCATTGGCCTAt   >  6:359014/1‑135 (MQ=255)
                                                                                                                              |                                                                                                                                       
TACGGCAAGATCAAACTCGGTGCCGACCACGACGAACCCGAGTTCAGCTACCTGTCGTGGGCCGGCATGCTGTTCGCCGCCGGCATCAGCATCACGTTGTTCTTCTTCTGTGTCTCCGAACCGCTGACCCACATGCTGCAACCGCCGCAAGGCGAAGCCGGCACGGTCGAGGCCGGGCGCCAGGCGATGCAGATTCTGTTCCTGCACTGGGGCCTGCATGGCTGGGGAGTGTTCGCCTTCGTCGGCATGGCATTGGCCTATT  >  KT2440/275832‑276093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑