Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,349,843	T→G	24.7%	V239G (GTC→GGC)	btuD_8 →	Vitamin B12 import ATP‑binding protein BtuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,349,843	0	T	G	24.7%	53.6 / 3.0	54	V239G (GTC→GGC)	btuD_8	Vitamin B12 import ATP‑binding protein BtuD
Reads supporting (aligned to +/- strand):  ref base T (4/35);  new base G (4/11);  total (8/46)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCCCAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCG  >  KT2440/4349708‑4349971                                                                                                                                        |                                                                                                                                   aCGCCCAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCCGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGCCGCCGGcgt                                                                                                                                    >  8:117007/1‑136 (MQ=255)     cccggATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGcgtcgt                                                                                                                                 <  1:147904/132‑1 (MQ=255)                gcgcgcgGGGGGCCTACCACCTGGTGTTGGTGCAACACGGCCGGGCGAGGGCCAGCGGCCGGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCGGGCCCTGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTa                                                                                                                     <  5:181090/134‑1 (MQ=255)                  gcgcgGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCCGGGCGACGCCGCCGGCGGCGG‑GTTGGgagggagg                                                                                                                   >  6:142112/1‑135 (MQ=255)                    gcgGGTGGCCGACCACCTGTTGTTGGTGGAACAAGGCCGGGCGAGCGCACGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCTGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGggt                                                                                                                 <  4:181137/135‑1 (MQ=255)                    gcgGCTGGCCGACCACCTGGTGTTGCTGGAACACGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCAGGCGTCGT‑GTTCGAAGGTAGggt                                                                                                                 <  5:132212/135‑1 (MQ=255)                      ggCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGGCGT‑GTTCGAAGGTAGggt‑ggg                                                                                                             >  8:319789/1‑135 (MQ=255)                              gACCCCCGGGTGTTGGGGCACCACGGCGGGGCTAGCCCCAGGGCCCCGAGCGGCTAAACCCTGGCGCGCTTTGACCTGTCGCTGGCCCAGGGCGCCGACGCCGGCGGCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCAc                                                                                                      <  4:255919/135‑1 (MQ=255)                              gACCACCGGGGGTTGCTGGACCACGGCGGGGCGATCGCCAGCGGCCCGAGCGGCGAAACCCTGGCGCGCCTTGACCTGTCGCTGGCCCCGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGG‑GGTTGGGCAc                                                                                                      <  5:129417/135‑1 (MQ=255)                                       gtgtTGCTGGAACACGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGGCGCGGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCAtt                                                                                            <  7:44193/136‑1 (MQ=255)                                       gggtTGCGGGAACCAGGCCGGGCTAGCGCCAGGGGCCGGAGCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCCGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCAtt                                                                                            <  7:290854/134‑1 (MQ=255)                                        tgtgGCTGGAACACGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCAtt                                                                                            <  5:330908/135‑1 (MQ=255)                                        tgtTGCTGGACCACGGCCGGGCGAGCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTGGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTa                                                                                           <  7:56237/136‑1 (MQ=255)                                        tgtTGCTGGAACACGGCCGGGCGAGCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCAtt                                                                                            <  2:50608/135‑1 (MQ=255)                                        tgtTGCGGTAACACGGCCGGGCGACCGCCCGCGGCCCGATCGGCGAAACCCGGGCTCGCCTTGACCTGTCGCTGGCCCCGGGCGACGACGCCGGCGGCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCAtt                                                                                            <  1:361083/135‑1 (MQ=255)                                        gggtGCGGGAACACGGCCGGGCGAGCGCCCGCGGCCCGAGCGGCGAAACCCTGGCTCGCCTTGACCTGGCGCTGGCCCAGGGCGACGACGCCGGCGGCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCAtt                                                                                            <  5:141106/132‑1 (MQ=255)                                         gtTGCTGGAACAAGGCCGGGCGAGCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTa                                                                                           <  2:59690/135‑1 (MQ=255)                                         gtTGCTGCAACAAGGCCGGGCGAGCGCCCGCGGCCCGCTCGGCGAAACCCTGCCTCGCCTTGACCTGTCGCGGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTa                                                                                           <  2:250855/135‑1 (MQ=255)                                             cTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACCCCGGCGGCGG‑GTTAGTAGGTAGGGTGGGTTGGGCCCCCCCCCCATAACgc                                                                                        >  2:159675/1‑134 (MQ=255)                                                gAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGGCGTGGTGGGATGTTGGGGT‑GTTTGGCCACGCCCCGCATACCGGCctc                                                                                    >  8:435372/1‑135 (MQ=255)                                                   cAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTACGGCctgctg                                                                                 <  3:213777/135‑1 (MQ=255)                                                   cAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTACGGCctgctg                                                                                 <  3:221099/135‑1 (MQ=255)                                                   cAAGGCCGGGCGATCGCCAGCGGCCCGAGCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTACGGCctgctg                                                                                 <  5:205611/135‑1 (MQ=255)                                                    aaGGCCGGGAGATCGACTGCGGCCAGATCGTCTAAACCATGTCTCGCCTTGACCTGTCGCTGGCCCTGGGCGACGACGCCTGCGTCGT‑GTTCGAATGTAGGGT‑GGTTTGTCACGACCCGCATTACGGCCTGCTgg                                                                                <  4:271910/135‑1 (MQ=255)                                                     aGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGATGACGCCGGCGTCGG‑GGTGGAAGGGAGGGG‑GGGTGGGGCCGCCCCGCAATACGGCCGGCgggc                                                                               >  5:154923/1‑134 (MQ=255)                                                              gATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGCCGCCGGCGTCGG‑GGTCGAAGGGTGGGT‑GGGTGGGGGCGCCCCGCCTTTCGGCCGGCGGGGCCTGccccg                                                                      >  2:313759/1‑134 (MQ=255)                                                                     cgCGGCCCGATCGGCGAAACCCTGGCTCGCGTTGACCTGGCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGgc                                                               <  4:103398/134‑1 (MQ=255)                                                                      gCGGCCCGCGCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGgcag                                                             <  7:370600/136‑1 (MQ=255)                                                                            cGATCGGCGAAACCCTGGCTCGCCTTGACCTGGCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCg                                                        <  4:200359/135‑1 (MQ=255)                                                                              ctCGGCCAACCCCGGGCGCGCGGTCCCCTGGGGCTGGCCCCGGGCGACGCCGCCGGCGGGGG‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCggg                                                      <  1:385366/134‑1 (MQ=255)                                                                              agcGGCCAACCCCTGCCTCGCGTTCACCTGGCGCGCGCCCGGGGCGACGACGCCGGCGTCGT‑GTTCGACGGTAGGGT‑GGGGGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCggg                                                      <  3:55607/133‑1 (MQ=255)                                                                                cGGCGAAACCCTGGCTCGCCTTGACCTGGCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGGAGGGT‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCg                                                   <  7:319789/136‑1 (MQ=255)                                                                                  gggAAACCCTGGCGCGCCTGGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTGCGAAGGTAGGGT‑GGTTGCGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCgc                                                  <  5:164403/133‑1 (MQ=255)                                                                                   cGAAACCCGGGCGCGCCTTGACCTGGCGCGGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGGAGGGG‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCgct                                                 <  4:345614/135‑1 (MQ=255)                                                                                               gcGCCTTGCCCTGTCGCTGGCCCCGGGCGACGACGCCGGCGTCGG‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCAc                                     <  3:406806/134‑1 (MQ=255)                                                                                                cGCCTTGACCTGGCGCGGGCCCCGGGCGCCGACGCCGGCGTCGG‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCAccc                                   <  7:377259/136‑1 (MQ=255)                                                                                                cGCCTTGACCTGGCGCGGGCCCCGGGCGACGACGCCGGCGTCGT‑GTTCGACGGTAGGGG‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCAcc                                    <  6:92835/135‑1 (MQ=255)                                                                                                 gCCTGGACCGGTCGCAGGCCACGGGCCCCGACGCCGGGGGCGT‑GTGCGAAGGTAGGGT‑GGGTGGGCACGACCCGCATTACGGCCTGCGGGACATGCGCCTGCCCGGCAGCAGCGGGCCGCTGCGGCGCATCAccc                                   <  6:330773/135‑1 (MQ=255)                                                                                                       aCCTGGCGCGGGCCGTGGGCGAAGAGGCCGGCGTCGT‑GTGCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTCCGGCCTGCTGGACCTGCGCCTGCGCGGCTGCAGCGGGCCGCTGCTGCGCATCACCCACgcgg                             <  6:351143/135‑1 (MQ=255)                                                                                                        ctgggcgcgGGCCCTGGGCTCCGACGGCGGCGTTGT‑GTTCGATGGTCGGGT‑GGTTGGGCCCGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACgcgg                             <  6:368595/129‑1 (MQ=255)                                                                                                            gcgcgGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGACGGTCGGGG‑GGGGGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAg                        <  6:205201/134‑1 (MQ=255)                                                                                                            gcGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTCGGGG‑GGTTGGGCACGCCCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAg                        <  3:114061/134‑1 (MQ=255)                                                                                                             cggagccccAGGGCGACGAGGCGGGCGTCGT‑GTTCGAAGGTCGGGT‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAg                        <  5:211096/128‑1 (MQ=255)                                                                                                             cGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTGGAAGGTAGGGT‑GGTGGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAg                        <  4:187607/134‑1 (MQ=255)                                                                                                              gcgggCCCCGGGCGACGCCGCCGGCGGCGT‑GTCCGACGGGAGGGT‑GGGTGCGCACGACCCGCATTACGGCCTGCGGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCGGCTGCGCATCACCCACGCGGCGCAGGt                      <  5:109345/132‑1 (MQ=255)                                                                                                              gcggcccccGGGCGACGACGCCGGCGGCGT‑GTGCGAAGGTAGGGG‑GGTGGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGt                      <  3:118054/130‑1 (MQ=255)                                                                                                              gcggcccccGGGCCACGACGCCGGCGGCGT‑GTGCGATGGACGGGG‑GGGTGGCCACGACCCGCATTACGGCCTGCTGGACCTGCGCCGGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGt                      <  6:287628/130‑1 (MQ=255)                                                                                                              gcggccccAGGGCGACGCCGCCGGCGGCGT‑GTGCGAAGGTAGGGT‑GGGGGGGCACGACCCGCATTCCGGCCTGCTGGCCCGGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGt                      <  2:4267/130‑1 (MQ=255)                                                                                                              gCTGGCCCCGGGCGACGACGCCGGGGGGGG‑GTTCGAAGGTAGGGT‑GGTGGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCGGCGCATCACCCACGCGGCGCAGGt                      <  4:160786/135‑1 (MQ=255)                                                                                                                    cccgggCGCCGACGCCGGCGGCGT‑GTGCGACGGTAGGGG‑GGGTGGGCACGACCCGCATTACGGCCGGCTGGCCCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATggg                <  5:64067/132‑1 (MQ=255)                                                                                                                    ccAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGG‑GGGGGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATggg                <  3:190627/135‑1 (MQ=255)                                                                                                                    ccAGGGCGACGACGCCGGCGTCGT‑GGTCGAAGGTTGGGT‑GGTTGGGCACGCCCCGCCATACGGCCGGCGGGGCCAGCGCCGGCCCCGCACCCGCGGGCCGCTGCAGCGGCACACCCCCCCGGCGGAGGGGATgggg               >  8:362405/1‑135 (MQ=255)                                                                                                                        ggCGCCGCCGCCGGCGTCGG‑GTTCCACGGGCGGGT‑GGTTGGCCACGACCCGCTTTACGCCCGGCTGGCCCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGgcagc            <  1:159675/135‑1 (MQ=255)                                                                                                                         gCGACGACGCCGGCGTCGT‑GTTGGAAGGGTGGGG‑GGTTTGGGACGCCCCCCAATTCGGGCTGCTGGACCAGCGCCGGCCCCGCCCCCGCGGGCCGCGGGTGCGCAGACCCCACCCCGCCCAGGGGCTggggcgccc          >  8:425573/1‑130 (MQ=255)                                                                                                                          cgccgacgCCGGCGTCGT‑GTTCGAAGGTCGGGG‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCAc          <  5:449847/132‑1 (MQ=255)                                                                                                                                 gCCGGCGTCGT‑GTGCGAAGGGAGGGT‑GGTGGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGcgcg  <  8:264759/136‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   ACGCCCAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGT‑GTTCGAAGGTAGGGT‑GGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCG  >  KT2440/4349708‑4349971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑