Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,369,407 A→C 18.5% P36P (CCT→CCG)  CJ019_04114 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,369,4070AC18.5% 127.7 / 6.0 100P36P (CCT→CCG) CJ019_04114hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (34/40);  new base C (9/13);  total (43/57)
Fisher's exact test for biased strand distribution p-value = 8.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

CCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCG  >  KT2440/4369278‑4369539
                                                                                                                                 |                                                                                                                                    
ccGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCGCGTACTCCCCCGCCCgcgc                                                                                                                                 >  1:179761/1‑135 (MQ=255)
ccGATCAGCAGAGCAGTGACGGGGCGGAAGCTCCTGGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAAGCGCACACCACCTGAAACAGCGACTCGGCCCGCAGGCGCAGGCGCTCGATCTCCGCCGCAGgcgcg                                                                                                                                <  7:62157/136‑1 (MQ=255)
     cagcagATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTc                                                                                                                            >  8:310903/1‑135 (MQ=255)
     cagcagATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCGCGATCGCCCCCGCCGGCCCGcccc                                                                                                                            >  6:282918/1‑133 (MQ=255)
        cagATCAGTGAAGGGGCGGAAGCGCCTTGCCCCGCATCTTGGCAATCACCCGACGCTGGAAATCGCACACCACCTCAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGAt                                                                                                                         <  6:173305/135‑1 (MQ=255)
          gagcAGTGACGGGGCGGAAGCTCCTGGCCCCGCATCTTGGCAACCACCCGAAGCGGGAAATCGGACACCACCTCAAACAGCGCCTCGGCCAGCAGGCGCAGGCGCTCGATCGCCGCCGCCGGCGCGCCTCGATccc                                                                                                                      <  8:212309/133‑1 (MQ=255)
           aTCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCCCCGCGAGccc                                                                                                                      >  1:249017/1‑135 (MQ=255)
              agtcacggggCGGAGGCTCCTTGCCCCGCATATTGCAAATCCCCCGACGCTGGAAAGCGCACACCCCCTGAAACAGAGACTCGGCCCGCAGGCGCAGGCGCTCGATCTCCGCCGCCGGCGCGCCTCGATCCcgc                                                                                                                    <  2:55843/128‑1 (MQ=255)
                  caGGGGCGGAAGCGCCTGGCCCCGCATCTTGGCAATCACCCGAAGCTGGAAATCGGACACCACCTGAAACAGAGACGCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTtg                                                                                                              <  8:270862/135‑1 (MQ=255)
                   cggggCGGAAGCTCCTTGCCCCGCATCTTGGCAATCCCCCCAAGCTGGTAAGCGGACACCACCTGAAACAGAGACTCGGCCAGCTGGCGCAGGCGCTCGATCTCCGCCGCCGGCGCGCCTCGATCCCGCGCCTtg                                                                                                              <  2:114952/134‑1 (MQ=255)
                   aGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGCCACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTtg                                                                                                              >  4:108249/1‑135 (MQ=255)
                   aGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCGCGCGCCCGCGCCTtg                                                                                                              >  1:12838/1‑135 (MQ=255)
                   aGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCACCTCGCTCCCGCGCCTtg                                                                                                              >  2:271232/1‑135 (MQ=255)
                    ggggggGATGCTCCTTGCCCCGCATCGTGGCAAGCCCCCGAAGCTGTTAAGCGGACACCACCTGAAACAGAGACTCGGCCAGCTGGCGCTGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTtgtg                                                                                                            <  8:337641/136‑1 (MQ=255)
                     gggCGGACGCGCCTTGCCCCGAATCTTGGCAACCACCCGAAGCTGGAAATCGCACACCACCTGAAACCGAGACGCGGCCAGCCGGCGCAGGCGCTCGAGCTCCGCCGCCGGCGCGCCTCGATCCCGCGCCTtgtg                                                                                                            <  2:113557/135‑1 (MQ=255)
                     gggCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCCGGCGCGCCTCGATCCCGCGCCGTGTGa                                                                                                           >  7:55927/1‑136 (MQ=255)
                     gggCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCGCGCTCCCGCGCCGTGTGa                                                                                                           >  7:212309/1‑136 (MQ=255)
                     gggCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTACGCCGCAGGCCCCGCAAGATCCCCCGCCTTGTGa                                                                                                           >  8:70622/1‑136 (MQ=255)
                     gggCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATATCCGCCGCAGGCGCGCCGCGCTCCCGCGCCGtggg                                                                                                            >  6:398720/1‑133 (MQ=255)
                        cGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGCTCCCGCGCCGTGTGATa                                                                                                         >  7:100191/1‑135 (MQ=255)
                        cGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCGTGTGATa                                                                                                         >  1:114952/1‑135 (MQ=255)
                        cGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCACGATCCCCCGCCTTGTGAt                                                                                                          >  3:151797/1‑134 (MQ=255)
                        cGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCCCTCTCCGCCGCCCGCCCCCCGAGCTCCCGCGCCGTGGGATaa                                                                                                        >  7:182279/1‑135 (MQ=255)
                           aaGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGCTCTCCGCCGCCGGCGCGCCGCGATCCCCCCCCTTGTGATACTc                                                                                                      >  5:352227/1‑135 (MQ=255)
                             gCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGGAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCGTGTGATACTccc                                                                                                    >  4:273961/1‑135 (MQ=255)
                             gCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTGCGCCGCCTGCGCGCCTCGCTCCCGCGCCTTGTGATACTccc                                                                                                    >  4:370778/1‑135 (MQ=255)
                             gCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCCGGCGCGCCGCGCTCCCCCGCCGTGTGATACGccc                                                                                                    >  1:113557/1‑135 (MQ=255)
                             gCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGGGCCGAGCTCCCCCGGCGTGTGAtgcagac                                                                                                    >  1:171880/1‑129 (MQ=255)
                             gCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCGCGATCTCCGCCGCAGGCCCCCCACCCTCCCGCCCCATGTGGTACTCCCg                                                                                                   >  7:47231/1‑136 (MQ=255)
                                            tCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGCTCCCGCGCCGTGTGCTTCTCCCGCATGGCGTCGCCAg                                                                                     >  3:24150/1‑135 (MQ=255)
                                             cTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCCGGCCCGCCGCGATACCGCGCCGTGTTATACTACCGCCAGGGGTCGCCAGc                                                                                    >  5:306243/1‑135 (MQ=255)
                                             cTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGCTCCCGCGCCTTGTGATACTCCCGCATGGCCTCGACCGc                                                                                    >  1:289564/1‑135 (MQ=255)
                                                   aaTCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCCCCTCGATCCCGCGCCGTGTGATACTCCCCCATGGCGTCGACAGCctgcg                                                                               >  4:277471/1‑133 (MQ=255)
                                                             cgCTGGTAACCGCCCCCCACCTGAACCCGAGCCGCGGCCCGCCGGCGCCGGCGCTCGAGCGCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGc                                                                    <  5:149762/134‑1 (MQ=255)
                                                                  ttAATCGCACACCCCCTCAAACAGCCACTCGGCCAGCAGGCGCCGGCGCTCGATCTCCGCCGCAGCCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGcc                                                               <  7:5886/134‑1 (MQ=255)
                                                                  gTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGcc                                                               >  1:121849/1‑135 (MQ=255)
                                                                    aaccGGACACCACGTGAACCACTCTCGCGGCCCGCTGGGGCAGGCGCTCTATCTCGGCGCCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAACCTGCTGAATCAGCGGCTCGCCGGc                                                            <  7:36208/133‑1 (MQ=255)
                                                                    aaTCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCgg                                                             >  4:127620/1‑135 (MQ=255)
                                                                     aTCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGCTCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGc                                                            >  4:395891/1‑135 (MQ=255)
                                                                      tCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCGCGATCCCCCGCCTTGTGATACTCCCGCATGGCGTCGACCGCCAGCTGAATCAGCGGCTCGCCGGcc                                                           >  2:341767/1‑135 (MQ=255)
                                                                        ggACCCCACCCCACACAGAGACGCGCCCCCCAGGCGCTGGCGCGCTATCTCCGCCGCCGGCGCGCCGCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTc                                                         <  1:332707/135‑1 (MQ=255)
                                                                        gccccccactTGAAACCGAGACGCGGCTACCTGCCGCTGGCGCTCGAGCTCGGCCGCAGCCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTc                                                         <  5:303993/130‑1 (MQ=255)
                                                                          acacCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGCCAGCCTGCTGAATAAGCGGCTCCCCGGCCTCgt                                                       >  2:133273/1‑134 (MQ=255)
                                                                          acacCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGAc                                                      >  8:48400/1‑136 (MQ=255)
                                                                          acacCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCGCGAACCCCCGCCCTGTGAATATACCGCCTGGCCGAGACCCCCAGCTGAATCCGCGGCAACCCGGCCTCgc                                                       >  6:269611/1‑134 (MQ=255)
                                                                           cccccccTGAAACAGACCCGCGGCCCGCCGGCGCAGGCGCTCGAGCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGAc                                                      <  5:102241/130‑1 (MQ=255)
                                                                           caccacCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGAc                                                      <  4:373305/135‑1 (MQ=255)
                                                                           caccacCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGAc                                                      <  4:367141/135‑1 (MQ=255)
                                                                               ccatcaaaCAGATACTCGGCTAGAAGTCGTCGGTGGTATTTCTCATCCGCTGGCGCGCCTCGATCCCGCGCATTGTGATACTCCCGCATGGCGTAGACAGCATGCTGAATCAGCTGCTCGCCGGAATCTACCATTc                                                 <  8:220380/131‑1 (MQ=255)
                                                                                 ctcaaaCAGAGACTCGCCCAGCCGGCGACGGCGCTCGATCCCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTcc                                                <  5:414064/132‑1 (MQ=255)
                                                                                   gAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGCTACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCCTTCCGa                                              >  2:127829/1‑135 (MQ=255)
                                                                                   gAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCGCGCTCCCGCGCCTTGGGATTCAACCGCAAGGCCTGGCCCGCCGGCGGAAACAACCGCTCGCCCGCCGCGccccatac                                                >  2:396242/1‑128 (MQ=255)
                                                                                        cgagaCTCGGCCCGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAg                                         <  1:60847/134‑1 (MQ=255)
                                                                                         gagaCTCGGCCAGCAGGCGCAGGCGCTCGAGCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGt                                       <  7:154363/136‑1 (MQ=255)
                                                                                           gccgcGGCCAGCAGGCGCAGGGGCGCTATCTCGGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTgc                                     <  7:70622/132‑1 (MQ=255)
                                                                                           gaCTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTgc                                     <  8:208405/136‑1 (MQ=255)
                                                                                            cggcGGCCCGCTGGCGCTGGCGCTCTAGCTCCGCGGCCGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTgc                                     <  3:40741/132‑1 (MQ=255)
                                                                                            aCTCGGCCCGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTgc                                     <  2:313124/135‑1 (MQ=255)
                                                                                             cTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCCGGCGCGCCTCGCTCCCCCGCCGTGTGATACTCCCCCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTgcg                                    >  6:359348/1‑135 (MQ=255)
                                                                                              tCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGTATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTgcgc                                   >  3:272257/1‑135 (MQ=255)
                                                                                              tCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGTATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTgcgc                                   >  6:124309/1‑135 (MQ=255)
                                                                                                 gCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATTCTCCCGCATGGCGTCGCCAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGCTGAAGGTGCGCTTg                                >  3:78901/1‑135 (MQ=255)
                                                                                                 gCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGt                               >  8:336079/1‑136 (MQ=255)
                                                                                                 gCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTACCGCATGGCGTCGACAGCCAGCTGAATCAGCGGCTCGCCGGCCTCCACCCTTCCGATGAAGGTGCGCATg                                >  8:389500/1‑135 (MQ=255)
                                                                                                   ccgccggcgccggcccGCGAGCCCCCCCCCCGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTc                              <  3:283357/130‑1 (MQ=255)
                                                                                                        gtcgccggggcGCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAat                         <  4:424868/133‑1 (MQ=255)
                                                                                                        ggcgctggcgctCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAat                         <  4:151797/135‑1 (MQ=255)
                                                                                                        ggcgccggcgcGCGACCGCCCCCGCCGCCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAat                         <  3:424208/135‑1 (MQ=255)
                                                                                                        ggcgcaggcgcTCGATCTCCGCCGCTGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAat                         <  4:64738/135‑1 (MQ=255)
                                                                                                          cgcaggcgcTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAatgcc                      <  7:310903/136‑1 (MQ=255)
                                                                                                            caggcgcTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAatgccat                    <  7:124219/136‑1 (MQ=255)
                                                                                                             tggcgcTCGAGCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAatgccat                    <  2:382747/134‑1 (MQ=255)
                                                                                                             aggcgcTCGAGCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAatgccat                    <  2:73669/135‑1 (MQ=255)
                                                                                                                  cggtaTCTCCGCCGCCGTCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCAGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTg              <  7:78671/132‑1 (MQ=255)
                                                                                                                  cTCGATCTCCGCCGCCGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTg              <  7:436879/136‑1 (MQ=255)
                                                                                                                  cTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTg              <  7:336079/136‑1 (MQ=255)
                                                                                                                     cccccccgcccCCGCCGCGCCTCGAGCCCGCGCCTTGTGATCCTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTg              <  2:205172/128‑1 (MQ=255)
                                                                                                                           cgccgcAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGtt       <  8:102225/134‑1 (MQ=255)
                                                                                                                            gccgccGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGtt       <  2:160129/133‑1 (MQ=255)
                                                                                                                            gccgccGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGAt    <  8:47231/136‑1 (MQ=255)
                                                                                                                            gccgccGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGAt    <  8:316726/136‑1 (MQ=255)
                                                                                                                            gccgcAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGtt       <  3:202957/133‑1 (MQ=255)
                                                                                                                            gccgcAGGCGCACCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGtt       <  1:271232/133‑1 (MQ=255)
                                                                                                                             ccgccgGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGAt    <  1:400474/135‑1 (MQ=255)
                                                                                                                             ccgcAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGAt    <  4:24150/135‑1 (MQ=255)
                                                                                                                             ccgcAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGAt    <  4:405081/135‑1 (MQ=255)
                                                                                                                             ccgcAGGCGCGCCTCGATCCCGCGCCGTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGAt    <  6:190913/135‑1 (MQ=255)
                                                                                                                              cgcgGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  7:163418/136‑1 (MQ=255)
                                                                                                                              cgcTGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  8:100191/136‑1 (MQ=255)
                                                                                                                              cgcCGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  7:225189/136‑1 (MQ=255)
                                                                                                                              cgcCGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  8:182279/136‑1 (MQ=255)
                                                                                                                              cgcAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  7:48400/136‑1 (MQ=255)
                                                                                                                               gcAGTCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  3:266849/135‑1 (MQ=255)
                                                                                                                               gcAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  3:139625/135‑1 (MQ=255)
                                                                                                                               gcAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  6:306243/135‑1 (MQ=255)
                                                                                                                               gcAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  6:387123/135‑1 (MQ=255)
                                                                                                                               gcAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  4:272257/135‑1 (MQ=255)
                                                                                                                               gcAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  3:273961/135‑1 (MQ=255)
                                                                                                                               gcAGCCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  2:202062/135‑1 (MQ=255)
                                                                                                                               ccAGCCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCg  <  6:371570/134‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                    
CCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAGGCGCAGGCGCTCGATCTCCGCCGCAGGCGCGCCTCGATCCCGCGCCTTGTGATACTCCCGCATGGCGTCGACAGCCTGCTGAATCAGCGGCTCGCCGGCCTCGACCATTCCGATGAAGGTGCGCTTGTCCAAATGCCATGCCTTGTTCAGTTGATCG  >  KT2440/4369278‑4369539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑