Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,750,123	C→T	5.7%	Q573* (CAG→TAG)	macB →	Macrolide export ATP‑binding/permease proteinMacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,750,123	0	C	T	5.7%	225.1 / 2.7	70	Q573* (CAG→TAG)	macB	Macrolide export ATP‑binding/permease proteinMacB
Reads supporting (aligned to +/- strand):  ref base C (40/26);  new base T (2/2);  total (42/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCGACATTGCCGTGGCGTTCGCCC  >  KT2440/4749993‑4750255                                                                                                                                   |                                                                                                                                     gTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAgcgc                                                                                                                                 >  7:446478/1‑136 (MQ=255) gTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGAATCGGAGTGATGAACATCATGCTCATGACCGTGCACGACCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAccg                                                                                                                                  >  2:281285/1‑135 (MQ=255)      tGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACAt                                                                                                                            >  7:254159/1‑136 (MQ=255)        aTGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACAt                                                                                                                            <  4:69050/134‑1 (MQ=255)            tCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACAtcctc                                                                                                                        <  6:184762/134‑1 (MQ=255)               gggCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCg                                                                                                                      >  4:57914/1‑133 (MQ=255)               gggCCATTGCCGCCATCTCGCAGCTGGTCGGCGGTTTCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCg                                                                                                                      <  2:390299/133‑1 (MQ=255)                   cATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCTCGAGCGCTCCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCCGTTc                                                                                                               >  7:181412/1‑136 (MQ=255)                        ccgccATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGa                                                                                                           >  2:435109/1‑135 (MQ=255)                           ccATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCg                                                                                                        <  5:99234/135‑1 (MQ=255)                             aTCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAgg                                                                                                     >  8:85020/1‑136 (MQ=255)                                  gctgctGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCg                                                                                                   <  8:385373/133‑1 (MQ=255)                                  gctgctGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGa                                                                                                  >  5:58769/1‑134 (MQ=255)                                     gctgGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCAt                                                                                              >  2:3641/1‑135 (MQ=255)                                               ggTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATgg                                                                                   <  8:107972/136‑1 (MQ=255)                                                           atgaACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTg                                                                        >  5:316834/1‑135 (MQ=255)                                                              aaCATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGAcc                                                                     >  3:209673/1‑135 (MQ=255)                                                              aaCATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGAcc                                                                     >  2:119980/1‑135 (MQ=255)                                                              aaCATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGAcc                                                                     >  1:6247/1‑135 (MQ=255)                                                              aaCATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGAcc                                                                     >  1:128084/1‑135 (MQ=255)                                                              aaCATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCg                                                                    >  8:111523/1‑136 (MQ=255)                                                              aaCATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCg                                                                    >  7:177341/1‑136 (MQ=255)                                                              aaCATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCg                                                                    <  8:16326/136‑1 (MQ=255)                                                                        tCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGt                                                                                         <  4:402278/105‑1 (MQ=255)                                                                        tCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGt                                                                                         >  3:402278/1‑105 (MQ=255)                                                                        tCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccc                                                           >  2:165720/1‑135 (MQ=255)                                                                        tCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccc                                                           >  2:122259/1‑135 (MQ=255)                                                                        tCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGAACGGCATCgccct                                                          >  8:94207/1‑136 (MQ=255)                                                                         cATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccct                                                          >  2:3413/1‑135 (MQ=255)                                                                             aCCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCg                                                                    <  4:213537/121‑1 (MQ=255)                                                                             aCCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCg                                                                    >  3:213537/1‑121 (MQ=255)                                                                              ccGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccctggcc                                                      <  2:121662/134‑1 (MQ=255)                                                                               cGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCAGGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccctggccctg                                                   >  7:172500/1‑136 (MQ=255)                                                                                gTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccctggccctg                                                   >  1:59901/1‑135 (MQ=255)                                                                                  gcgcGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCtggt                                                 <  4:284260/135‑1 (MQ=255)                                                                                    gcgAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCTAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCtggtgg                                               >  2:274813/1‑135 (MQ=255)                                                                                    gcgAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCTAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCtggtgg                                               >  2:267872/1‑135 (MQ=255)                                                                                    gcgAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGCCCGGCATCGCCCTGGCCCtggtgg                                               >  5:320483/1‑135 (MQ=255)                                                                                    gcgAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCtggtgg                                               >  3:219359/1‑135 (MQ=255)                                                                                    gcgAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCtggtgg                                               >  3:440876/1‑135 (MQ=255)                                                                                    gcgAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCtggtgg                                               >  4:374067/1‑135 (MQ=255)                                                                                    gcgAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCtggtgg                                               >  3:175499/1‑135 (MQ=255)                                                                                    gcgAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTggtggt                                              >  7:100653/1‑136 (MQ=255)                                                                                    gcgAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTggtggt                                              >  8:184457/1‑136 (MQ=255)                                                                                     cgAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTggtggt                                              >  4:299012/1‑135 (MQ=255)                                                                                      gAGCGCACCCGTGAAATCGGTATCCGCATGCCCACCGGCGCCCCCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGAGATCATGCTGTAGATGGTTCGCGTCCTGACCGGCATCGCCCTGGCCCAGGTGGTc                                             >  2:34806/1‑135 (MQ=255)                                                                                       aGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCgg                                           <  8:254159/136‑1 (MQ=255)                                                                                       aGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCgg                                           <  7:251604/136‑1 (MQ=255)                                                                                       aGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCgg                                           <  7:184457/136‑1 (MQ=255)                                                                                        gcgcCCCCGTGAACTGGGGATCCGCCTGGCCACCGGCGCCCGCCAGCGCGACAGCCACCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCGTGGTGGTCgg                                           <  5:289711/135‑1 (MQ=255)                                                                                        gcgcACGCGTGAACTCGGTATGCGGATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGGTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCgg                                           <  1:281285/135‑1 (MQ=255)                                                                                        gcgcACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGTCATCGCCCTGGCCCTGGTGGTCgg                                           <  4:127934/135‑1 (MQ=255)                                                                                        gcgcACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCgg                                           <  5:145974/135‑1 (MQ=255)                                                                                        gcgcACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCgg                                           <  1:34806/135‑1 (MQ=255)                                                                                        gcgcACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCgg                                           <  6:184934/135‑1 (MQ=255)                                                                                        gcgcACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCgg                                           <  3:178469/135‑1 (MQ=255)                                                                                                       cGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCt                            <  4:317683/135‑1 (MQ=255)                                                                                                       cGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGGCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCt                            <  6:54867/135‑1 (MQ=255)                                                                                                          tATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGc                         <  5:202021/135‑1 (MQ=255)                                                                                                           aTCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGc                         >  6:79059/1‑134 (MQ=255)                                                                                                           aTCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCg                       >  8:97640/1‑136 (MQ=255)                                                                                                             ccGCATGGCCACCGGCGCCCGCTAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCg                       <  1:274813/134‑1 (MQ=255)                                                                                                             ccGCATGGCCACCGGCGCCCGCTAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCg                       <  1:267872/134‑1 (MQ=255)                                                                                                             ccGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCg                       >  3:174967/1‑134 (MQ=255)                                                                                                             ccGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCg                       >  6:94598/1‑134 (MQ=255)                                                                                                               gCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGCCCGGCATCGCCCTGGGCCTGGTGGTCGGCGCCCGCCTTACCCTGGCCGACa                    >  2:222306/1‑135 (MQ=255)                                                                                                                   ggCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCGACATTGc                >  6:17278/1‑135 (MQ=255)                                                                                                                   ggCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCGACATTGc                <  4:174967/135‑1 (MQ=255)                                                                                                                     ccACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATAGCCCTGGCCCTGGTTGTCGGCGCCAGCCTTACCCTGGCCGACATTGCCg              >  6:384674/1‑135 (MQ=255)                                                                                                                       aCCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCGACATTGCCGTg            <  1:435109/135‑1 (MQ=255)                                                                                                                            cgcccgccAGCGCGACATCCTCCGGCAGTTCCTGCGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCCGCCTTACCCTGGCCGCCATTGCCGTGGCggt       >  6:413988/1‑133 (MQ=255)                                                                                                                             gcccgccAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCGACATTGCCGTGGCGTTc      <  3:232727/135‑1 (MQ=255)                                                                                                                             gcccgccAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCGACATTGCCGTGGCGTTc      <  3:232595/135‑1 (MQ=255)                                                                                                                              cccgccAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCGACATTGCCGTGGCGTTCGc    <  7:85020/136‑1 (MQ=255)                                                                                                                                 gccAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCGACATTGCCGTGGCGTTCGccc  <  1:122259/135‑1 (MQ=255)                                                                                                                                   |                                                                                                                                     GTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACCCTGGCCGACATTGCCGTGGCGTTCGCCC  >  KT2440/4749993‑4750255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑