Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,824,960 C→T 6.3% R18H (CGC→CAC)  lgrB_2 ← Linear gramicidin synthase subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,824,9600CT 6.3% 202.2 / 3.2 65R18H (CGC→CAC) lgrB_2Linear gramicidin synthase subunit B
Reads supporting (aligned to +/- strand):  ref base C (20/40);  new base T (2/2);  total (22/43)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-01

AAGCTGGCGCGCCCCTGCAAGGCGGCGGCGATGGTGCGTGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGCGACCGGGGAT  >  KT2440/4824825‑4825090
                                                                                                                                       |                                                                                                                                  
aaGCTGGCGCGCCCCTGCAAGGCGGCGGCGATGGTGCGTGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTgcgcggcgc                                                                                                                                    >  8:160979/1‑136 (MQ=255)
     ggCGCGCCCCTGCAAGGCGGCGGCGATGGTGCGCGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTACGGGGTCTGCGCGGCGCGTTgc                                                                                                                               <  7:344768/136‑1 (MQ=255)
      gcgcgcCCCTGCAAGGCGGCGGCGATGGTGCGTGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTgcg                                                                                                                              <  7:301597/136‑1 (MQ=255)
      gcgcgcCCCTGCAAGGCGGCGGCGATGGTGCGTGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTgcg                                                                                                                              <  8:26600/136‑1 (MQ=255)
      gcgcgcCCCTGCAAGGCGGCGGCGATGGTGCGTGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTgc                                                                                                                               <  4:14155/135‑1 (MQ=255)
      gcgcgcCCCTGCAAGGCGGCGGCGATGGTGCGTGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTgc                                                                                                                               <  5:356510/135‑1 (MQ=255)
       cgcgcCCCTGCAAGGCGGCGGCGATGGTGCGTGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTgcg                                                                                                                              <  6:386121/135‑1 (MQ=255)
       cgcgcCCCTGCAAGGCGGCGGCGATGGTGCGTGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTgcg                                                                                                                              >  3:214419/1‑135 (MQ=255)
                         cggcgATGGTGCGTGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCACGGCCTGGCCCAg                                                                                                              >  4:438081/1‑133 (MQ=255)
                          ggcgATGGTGCGTGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCga                                                                                                           <  5:329207/135‑1 (MQ=255)
                           gcgATGGTGCGTGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGCCCAGCgag                                                                                                          >  6:289101/1‑135 (MQ=255)
                                       gcACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTc                                                                                               >  5:40769/1‑134 (MQ=255)
                                       gcACGCTGGTCGAGGTCCCGGGAGCGTAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGTCAATCCTTTCCGGGGTCTGCGCTGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGTGAGTTCg                                                                                              <  2:111552/135‑1 (MQ=255)
                                        cACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTc                                                                                               >  1:163693/1‑133 (MQ=255)
                                         aCGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGTGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAg                                                                                           >  7:70885/1‑136 (MQ=255)
                                         aCGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGaa                                                                                            >  5:363675/1‑135 (MQ=255)
                                         aCGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCAGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAg                                                                                           >  7:336704/1‑136 (MQ=255)
                                          cGCTGGTCGAGGGCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTGGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAgg                                                                                          <  7:287770/136‑1 (MQ=255)
                                           gCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAgg                                                                                          <  5:121658/135‑1 (MQ=255)
                                           gCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAgg                                                                                          <  5:102351/135‑1 (MQ=255)
                                           gCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAgg                                                                                          <  4:81304/135‑1 (MQ=255)
                                           gCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAgg                                                                                          <  6:40769/135‑1 (MQ=255)
                                            cTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGc                                                                                         <  2:286463/135‑1 (MQ=255)
                                                 cGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGcgtccgt                                                                                   >  8:409338/1‑136 (MQ=255)
                                                       cccGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTg                                                                              <  3:335052/135‑1 (MQ=255)
                                                       cccGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTg                                                                              <  4:271774/135‑1 (MQ=255)
                                                       cccGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTg                                                                              <  2:163693/135‑1 (MQ=255)
                                                            tAGCTGAGCTCGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGagag                                                                                                        <  1:130759/104‑1 (MQ=255)
                                                            tAGCTGAGCTCGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGagag                                                                                                        >  2:130759/1‑104 (MQ=255)
                                                            tAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGtt                                                                        >  7:305724/1‑136 (MQ=255)
                                                             aGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGtt                                                                        >  1:157833/1‑135 (MQ=255)
                                                               cTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTcc                                                                      <  2:170943/135‑1 (MQ=255)
                                                               cTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCt                                                                     <  8:134218/136‑1 (MQ=255)
                                                                tGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCt                                                                     <  4:421056/135‑1 (MQ=255)
                                                                tGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGGCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCt                                                                     <  6:286918/135‑1 (MQ=255)
                                                                  aGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTg                                                                    <  8:346090/134‑1 (MQ=255)
                                                                   gCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTg                                                                    <  5:307083/133‑1 (MQ=255)
                                                                    cACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGTGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAg                                                                <  8:70885/136‑1 (MQ=255)
                                                                              tCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTgg                                                         <  2:252309/133‑1 (MQ=255)
                                                                              tCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGGCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTgg                                                         <  5:279386/133‑1 (MQ=255)
                                                                               cACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTa                                                      >  2:67940/1‑135 (MQ=255)
                                                                                 ccGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAgg                                                    <  5:26942/135‑1 (MQ=255)
                                                                                      tgtgtCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTgc                                                                                                                               >  7:444121/1‑55 (MQ=255)
                                                                                      tgtgtCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTgc                                                                                                                               <  8:444121/55‑1 (MQ=255)
                                                                                      tgtgtCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGa                                               >  8:113776/1‑135 (MQ=255)
                                                                                             gCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGCCCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAgctgc                                        >  1:121751/1‑135 (MQ=255)
                                                                                             gCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCATCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAgctgc                                        >  1:15203/1‑135 (MQ=255)
                                                                                              cGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAgctgct                                       <  6:220089/135‑1 (MQ=255)
                                                                                                   aaGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCt                                  >  8:313624/1‑135 (MQ=255)
                                                                                                    aGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTa                                 <  1:67940/135‑1 (MQ=255)
                                                                                                           ggCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCCAAGGCGTCCGTCAATGGGTGTTCCTGCCAGTGTCTGGGTAGGTCTGACAGCTGCTACGCTAACGTggg                          >  3:308935/1‑135 (MQ=255)
                                                                                                            gCACTCCTTTCCCGGGGCGGCGCGGCGCGTGGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGATAGGGCTGACAGCTGCTCCGCTAACGTgggc                         <  1:285658/135‑1 (MQ=255)
                                                                                                            gCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTgggc                         <  2:142945/135‑1 (MQ=255)
                                                                                                                        ggggTCTGCGCGGCGTGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCAt                                                                                >  3:380387/1‑68 (MQ=255)
                                                                                                                        ggggTCTGCGCGGCGTGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCAt                                                                                <  4:380387/68‑1 (MQ=255)
                                                                                                                        ggggTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGc            >  8:75627/1‑136 (MQ=255)
                                                                                                                         ggggcggcgcggcgggTTGCGCCAGGGCCGGGACCAGCGAGAGCGGGTGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGc            <  4:195548/129‑1 (MQ=255)
                                                                                                                         gggTCTGCGGGGGGGGTGGCGCCTGGGCCTGGACCATCGTGAGCGGGAGTTCTAAGGCGTCCGGCATGGGGTGGTGCTGGCTGGGTCTGGTTAGGTCTGACAGGGGCTCCGCTTCGGTGGGCGGGCAAGGTAGGc            <  6:135527/135‑1 (MQ=255)
                                                                                                                         gggTCGGCGCGGCGCGTGGCGCGAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGGCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGc            <  4:242152/135‑1 (MQ=255)
                                                                                                                          ggggtGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGGGGGCGGGCAACGTAGGCGa          <  7:160979/132‑1 (MQ=255)
                                                                                                                          ggTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCTGGGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGCGa          <  8:336704/136‑1 (MQ=255)
                                                                                                                          ggTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGCg           >  3:203005/1‑135 (MQ=255)
                                                                                                                          ggTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGCGa          <  8:336238/136‑1 (MQ=255)
                                                                                                                          ggTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTGCCTGCCAGTGGCGGGTTAGGGCTGACAGCGGCT‑CGCTAGCGTGGGCGGGCAACGTAGGCGa          <  4:18030/135‑1 (MQ=255)
                                                                                                                           ggcTGCGCGGCGCTGTGCGCAAGGGCCGGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGCGa          <  6:30734/133‑1 (MQ=255)
                                                                                                                           ggcTGCGCGGCGCGTGGCGCCAGGGCCTGGACCAGCGTGGGCGGGTGTTCGAAGGCGTCCTTCATTGGGTGTTCCTGCCAGTGTGTGGTTAGGTCTTACTGCTGCTCCGCTAACGTGGGCGGGCAACTTAGGCGa          <  4:308935/133‑1 (MQ=255)
                                                                                                                              tGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGCGACCg       <  2:114163/135‑1 (MQ=255)
                                                                                                                               gcgcggcgcgTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGTTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGCGACCggg     <  8:440492/136‑1 (MQ=255)
                                                                                                                               gcgcggcgcgTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGCGACCggg     >  7:104809/1‑136 (MQ=255)
                                                                                                                               gcgcggcgcgTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGCGGGCGGGCAACGTAGGCGACCggg     >  7:155137/1‑136 (MQ=255)
                                                                                                                               gcgcggcgcgTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGAGGGCGGGCACCGTAGGCGACCgg      >  1:313253/1‑135 (MQ=255)
                                                                                                                                cgcggcgggTTGCGCCTGGGCCTGGACCAGCGAGTGCGGGAGTTCGAAGGCGGCCGTCATTGGGTGTTCGTGCCAGTGTGTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGGGGGCAACGTAGGCGACCggg     <  6:363675/135‑1 (MQ=255)
                                                                                                                                cgcggcgcgTTGCGCCAGGGCCTGGACCAGGGAGAGCGGTCGTGCTAAGGCGTCCGTCATTGGGGGTGCCTGCCAGGGTCTGGTTAGGGCTGACAGCTGCTCGGCTAACGTGGGCGGGCAACGTAGGCGACCggg     <  6:446458/135‑1 (MQ=255)
                                                                                                                                cgcggcgcgTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGCGACCg       <  8:305724/133‑1 (MQ=255)
                                                                                                                                cgcggcgcgTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGGGGGCGGGCAACGTAGGCGACCggg     <  1:111735/135‑1 (MQ=255)
                                                                                                                                 gcggcgcgTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAgg                                                    <  6:124542/87‑1 (MQ=255)
                                                                                                                                 gcggcgcgTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAgg                                                    >  5:124542/1‑87 (MQ=255)
                                                                                                                                  cggcgcgTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGCGACCGGGGAt  <  8:447450/136‑1 (MQ=255)
                                                                                                                                  cggcgcgTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGCGGGCGGGCAACGTAGGCGACCGGGGAt  <  8:155137/136‑1 (MQ=255)
                                                                                                                                   ggcgcgTTGCGCCAGGGCCTGGACCAGCGTGAGCGGGAGTTCGACGGCGTCGGTCTTTGGGTGTTCCTGCCAGTGTCGGGTTAGGGCTGACAGCTGCTCCGCTAACGTGGGCGGGAAACGTAGGCGACCGGGGAt  <  6:157442/135‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
AAGCTGGCGCGCCCCTGCAAGGCGGCGGCGATGGTGCGTGCACGCTGGTCGAGGTCCCGGTAGCTGAGCACGGCCTGCTCACCGGGTGTGTCGGCGAGAAAGCGCAGGGCAATCCTTTCCGGGGTCTGCGCGGCGCGTTGCGCCAGGGCCTGGACCAGCGAGAGCGGGAGTTCGAAGGCGTCCGTCATTGGGTGTTCCTGCCAGTGTCTGGTTAGGTCTGACAGCTGCTCCGCTAACGTGGGCGGGCAACGTAGGCGACCGGGGAT  >  KT2440/4824825‑4825090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑