| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,845,478 | T→G | 46.3% | intergenic (+47/+72) | apt → / ← recR | Adenine phosphoribosyltransferase/Recombination protein RecR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,845,478 | 0 | T | G | 46.3% | 9.4 / 2.4 | 25 | intergenic (+47/+72) | apt/recR | Adenine phosphoribosyltransferase/Recombination protein RecR |
| Reads supporting (aligned to +/- strand): ref base T (2/10); new base G (6/5); total (9/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.94e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCTCCTACGAAAGCCGC‑GTATGTTTTGT‑A‑GGCGC‑GGGTTTACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGG > KT2440/4845350‑4845587 | ggAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGGAAAACCGCGccatcc > 8:259027/1‑131 (MQ=255) cTGGACGGGTCGCGGCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCTCCTACGAAAGc < 8:362067/136‑1 (MQ=255) ggggCGGGTGGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCTCCTACGAAAGCCGC‑Gt < 5:150449/135‑1 (MQ=255) gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAAACCCCGCCGACGAAAGCCCC‑GGATGtg > 1:82366/1‑134 (MQ=255) ggTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCTCCTACGAAAGCCGC‑GTATGTTTTGT‑a < 2:352467/135‑1 (MQ=255) ggTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCTCCTACGAAAGCCGC‑GTATGTTTTGT‑a < 5:196558/135‑1 (MQ=255) gggTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTTAACCCGCGCCGCCGTCAGCCGC‑GGATGTTTTGT‑T‑GGCGC‑GGGTGTGccc > 4:44051/1‑135 (MQ=37) cTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTTAAACCCCGCCGACACAAGCCGC‑CGATTTTTTGT‑T‑TGCGCGGGGTTAACCCCC‑CAATggg > 3:193979/1‑135 (MQ=255) tGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTTAAACCCCTCCGACCGAAGCCGC‑GGATGTTTTGT‑T‑TGCGC‑GGGTTTTCCCGC‑GAAATGGCCGGCaacc > 2:13585/1‑133 (MQ=255) ctaGTTTTCCCACCCGGAGTTTTGGGTGGGGTGTGGGGGCGGCTTGGCTGCCCATTCGCGGGAAAACCCGCACCAACCAACCCCGC‑TTAGGTTTTGT‑A‑GCCGC‑GGGTTAACCCGC‑GAATGGGCCGCCAAGCGGcc < 2:20320/133‑1 (MQ=255) gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTTAACCCCCTCCGAACCAACCCGC‑GGATGTTTTTT‑A‑GGCGC‑GGGGTTAACCGC‑GAATGGGCCCCCCAGCGGgcc > 1:370996/1‑135 (MQ=25) gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAAACCGCGCCGAACCAAGCCGCGGTTGGTTTTGT‑‑‑GGCGC‑GGGGTTTGCCGCGGAATGGGCC‑CCAACCGGcccc > 8:122252/1‑136 (MQ=255) ttCCCTCAGCGCGTATTGCGTGGGCTTTTGGGGCTGCTTGGCAGCCCATTCGCGGGTAAACCCGCTCCTCCGAAAGCCGC‑GTATGTTTTGT‑A‑GGCGC‑GGGTTTACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATg < 7:319588/135‑1 (MQ=255) cgccgCGAGTTTTGTGGGGGCTTTGGGGGGGGCTGGGCCCCCAATTCGCGGTAAACCCCGCGCCAACGACCCCCGC‑GTTTGTTTTGT‑A‑GGCGC‑GGGTTTACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATGAATc < 4:224065/131‑1 (MQ=16) cTCCGCGTGTTTTGCGTGGGCGTTTGGGGCTGCTTTGCAGCCCAGTCGGGGGTACACCCGCTCCTCCTAAAGCCGC‑GTAT‑TGTTGTGA‑GGCGC‑GGGTTTACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATGAATc < 6:83228/135‑1 (MQ=25) tccgCGAGTATTGCGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCTCCTACGAAAGCCGC‑GTATGTTTTGT‑A‑GGCGC‑GGGTTTACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATGAATCa < 2:80866/132‑1 (MQ=255) gcAGCGTGTTTTGCGGGGGCTTTTGGGGCTGCTTTGCAGCCAATGCGGGGGTAAACCCGCTCCTACGAACCCCGC‑GTATGTTTTGT‑A‑GGCGC‑GGTTTAACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATGAATCa < 1:13585/134‑1 (MQ=255) gggTGGGGTTGTGGGGGGGGTTTCCCGCCCATTGGGGGGTAAACCCGCGCCACCCAACCGCGG‑GTTTTTTTTGG‑ACGGCG‑‑GGGTTTACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGc < 8:17065/134‑1 (MQ=255) gTGGGCTGTGGGGGCGCCTGTCCCGCCCATGCGGGGGAAAACCCGCGCCTACCAAACCCCC‑GTATGTTTTGC‑C‑GGCGC‑GGGTTAACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCg < 7:306135/136‑1 (MQ=255) gggCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTTAAACCGCCCCGACGAAAGCCCG‑GGTTGTTTTTT‑T‑TGCGCGGGGTTTACCGCC‑GAATGGGCGCCCACCGGGCCCCAAAG‑ATAAGCGCGAAAAggggccgc > 8:389241/1‑131 (MQ=255) ggCTTTGGGGGCTGCTTTCCAGCCCTTTCGCGGGAAACCCCGCGCCACCCAACGCCGC‑GTATGTTTTGT‑C‑GGCGG‑GGTTTAACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGgc < 4:193979/135‑1 (MQ=255) tttGGGGCTGCTTTGCAGCCCATTCGCGGGTTAACCCGCGCCGTCGCAAGCCGC‑GGATGGTTTTT‑T‑GGCGC‑GGGGTTTACCGC‑GCATGGGGCCCCCAGCGGCCCCCATGGATCAACGCGCAATGGGGCCGGGccc > 2:87941/1‑135 (MQ=12) ggggCGGCTTTGCAGCCAATGCGCGGGTAAACCCGCGCATCCGAAAGCCGC‑GTTTGTTTTGC‑C‑GGCGC‑GGGTTTACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTg < 2:72178/134‑1 (MQ=38) ttccAGCCCATTCGCGGCTAACCCCGCTCCTACGACCGCCGC‑GTATGTTGTGT‑C‑GGCGC‑GGGTTTACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATg < 4:152020/132‑1 (MQ=38) cAGCCCATTCGCGGGTAAACCCGCTCCTACGAACGCCGC‑GTTTGTTTTGA‑C‑GGCGC‑GGGTTTACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATggg < 4:442537/134‑1 (MQ=255) | GGAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCTCCTACGAAAGCCGC‑GTATGTTTTGT‑A‑GGCGC‑GGGTTTACCCGC‑GAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGG > KT2440/4845350‑4845587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |