Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,874,567 A→C 31.2% intergenic (+13/‑174) CJ019_04568 → / → uacT_1 hypothetical protein/Uric acid transporter UacT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,874,5670AC31.2% 47.1 / 4.7 47intergenic (+13/‑174)CJ019_04568/uacT_1hypothetical protein/Uric acid transporter UacT
Reads supporting (aligned to +/- strand):  ref base A (11/20);  new base C (9/7);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCG  >  KT2440/4874439‑4874702
                                                                                                                                |                                                                                                                                       
ttCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc                                                                                                                                   <  4:297457/135‑1 (MQ=255)
  ccggCGCAAGCGTTTGCCAGCCAGATCAGCCCGCTGGTAAACATCACCCTTATCACCACCTAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc                                                                                                                                   <  5:229743/131‑1 (MQ=255)
        cAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGccccc                                                                                                                           >  2:371794/1‑135 (MQ=255)
            cGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACGGGCTCGCCAAAGGCGCCCCGGTCAACTGCCAGCACCTCGCCCGGCACCCCGCCCCCCCAg                                                                                                                       >  1:237014/1‑135 (MQ=255)
             ggTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCCGGTCAACTGACAGCCCCACGCCCGGCCACCCGCCCCCCCAgg                                                                                                                      >  5:447056/1‑135 (MQ=255)
                 gccagccaGATCATGCGCCTGGGCAACACCCCCCCGATGACCCCCGAAGCCCGCACGCGCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATc                                                                                                                 <  7:369914/136‑1 (MQ=255)
                   ctgccagATCATGCCGCTGGGAAAAATAACCCAGATCACCCCCGAAGCGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATc                                                                                                                 <  4:207602/132‑1 (MQ=255)
                   cagccagATCATGCGGCTGGGAAACATCACCCAGATCACCACCCAAGAGCCCACGCTCGGCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCa                                                                                                                <  2:156167/135‑1 (MQ=255)
                     gccagATCATGCCGCTGGCCAACATCACCAAGATCACAACCTATGAGCGCACGCTGGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCAcc                                                                                                              <  5:420390/135‑1 (MQ=255)
                     gccagAGCAGGCCGCGGGGCAACATCACCCAGATGACCACCGACGAGCGCACGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCAcc                                                                                                              <  2:12483/135‑1 (MQ=255)
                       cagaaaaTGCCGCTGCGAAAAACCCCCAAAACGACCACCTAACAGCCCATGTGCGTCGGCGACTGGATCGCCAAAGGCGCCCATGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCtt                                                                                                            <  3:148968/129‑1 (MQ=255)
                            cATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGTTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTg                                                                                                      >  8:131243/1‑136 (MQ=255)
                                          aCATCCCCCAGATCCCCCCCGAAGAGCGACCGCGGGCGGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCtt                                                                                         <  6:957/135‑1 (MQ=255)
                                              cACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGCATCCCCTTCCCCGGCAGGAGCGGGCTTGccc                                                                                     >  3:337563/1‑135 (MQ=255)
                                                   aGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCCGCAACCCGCCCCCCAAGGGCATCACCATCCCCGGCAGGCGCGGGCGTGCCcgc                                                                                   >  1:136951/1‑132 (MQ=255)
                                                      tGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAACTCACAGCACCTCGCCCGGCAACCCCCCCCCCAACGGAATCACCATCCCCTGCAGGCGCCGGCTTGCCCCCCAagag                                                                             >  2:250168/1‑135 (MQ=255)
                                                        accaccGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCCAGGGAATCACCTTCCCCTGCAGGAGCGGGCGTGGCCGCGCAGAGGc                                                                           >  3:58561/1‑135 (MQ=255)
                                                           accGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTGAAATGCCAGCACCGCGCCACGCAACCCCCCCCCCCAGGGAATGCCCATCCCCAGCAGGCGCCGGCGTTCCCGCGCAGAGGCCg                                                                         >  3:210285/1‑134 (MQ=255)
                                                             cGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCCAAGGCGCCCCGGTCAACAGACAGCCCCACCCCACGCCACCCCCCCCCCCACGGAATAACCATCCCCAGCAGGCGCCGGCATGCCCGCCGAGAGGCCGaaa                                                                      >  2:195249/1‑133 (MQ=255)
                                                                  agCGCAAGCTCGTCGGCGACTGGATCGCCCAAGGCGCCCCGGTCAACTGACAGCCCCACGCCCCGCCACCCCCCCCCCACCGGCATCACCATCCCCAGCAGGGGCGGGCGTGCCCGCGAAGAGGCCGCAAGcccc                                                                 >  4:265495/1‑135 (MQ=255)
                                                                  agCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCACGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCATCCCCAGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGcccc                                                                 >  6:162208/1‑135 (MQ=255)
                                                                      cAAGCGCGGCGGCGACTGGATCGCCAACGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCACCCCCCCCCCCAAGGGAATCACCTTCCCCTGCCGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGcc                                                             <  4:337563/135‑1 (MQ=255)
                                                                         gCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCAGCAGGCGCGGGCTTGCCCGCGCAGAGGCCGCTAGCCCCAGCCTcc                                                          >  5:197621/1‑134 (MQ=255)
                                                                               cgGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc                                                                                                                                   >  7:146244/1‑56 (MQ=255)
                                                                               cgGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc                                                                                                                                   <  8:146244/56‑1 (MQ=255)
                                                                                 gCGACTTGATCGCCAAAGGCGCCCAGGGCAAATGCCAGCACCTCGCCCGGCCACCCCCCCCCCAACGGAATCACCGTCCCCTGCAGGCGCGGGGTTGCCCGCGCAGCGGCCGATAGCCCCCGCCGCCAGCGCCaa                                                  >  4:216422/1‑134 (MQ=255)
                                                                                 gCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCCCCGTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGCGGCCGCTAGCCCCAGCCGCACGCTccct                                                  >  4:295187/1‑133 (MQ=255)
                                                                                 gCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCCACCCGCCCCCCAAGGGAATCACCATCCCCTGCAGGAGCGGGCTTGCCCCCGAAGAGGCCGATAGCCCCAGCCTCCAGCTCCAtg                                                 >  7:34650/1‑136 (MQ=255)
                                                                                    tcTGGAGCGCAAACGGCGCCCTGGTCAACTGACACCACCTCGCCAGGCACCCCGCCCCCCACGGTAATCCCCTTCCCCTGCTGGCGGGGGGTGGCCCGCTACGAGGCCGATAGCCCCAGCCTCAAGCTCCAtgtg                                               <  2:136951/134‑1 (MQ=255)
                                                                                        gATCCCCAAAGGCGCCCAGGTCCACAGACACCACCTCCCCACGCAACCCCCCCCCCAACGGAATCACCATCCCCTGCAGGCGCGGGCTTGCCCGCGAAGACGCCCATAGCCCCCCCCACAAGCTCCATCTGtttc                                           >  4:215281/1‑133 (MQ=255)
                                                                                         agccccAACGCCGCCCAGGTCAACTGACAGCACCCCCCCCGCCACCCCCCCCCCCAAGGCAATCACCTCCCCCTCCAGGCGCGGGCTGGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                          <  6:447056/131‑1 (MQ=255)
                                                                                         aTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCACCCCGCCCCCCAAGGTAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                          <  1:31617/135‑1 (MQ=255)
                                                                                         aTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGCGGGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                          <  1:14424/135‑1 (MQ=255)
                                                                                             ccAACGGCGCCCAGGTCAACTGACACCACCTCGCCAGCCAACCCCCCCCCCAAGGGAATCACCTTCCCCTGCAGGCGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac                                      <  6:99747/135‑1 (MQ=255)
                                                                                             caaaaGGCGCCCCGGTCAACGTACACCACCTCGCCCGGCAACCCGCCCCCCATGTAAATCCCCTACCCGGGCTGGCGCGGGCTTGCCGGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac                                      <  3:324425/133‑1 (MQ=255)
                                                                                                           gacAACTGACAGCACCGCGCCCGGCACCCCGCCCCCCACGGGAACCACCACCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                       <  7:11381/134‑1 (MQ=38)
                                                                                                            tCAACTCACAGCACCTCGCCAGGAAACCCGCCCCCCAGCGTAATCACCTTCCCGTGCAGGAGCGTGCTTGCCGGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                       <  3:421705/135‑1 (MQ=255)
                                                                                                            acAACTGACACCACCGCGCCCGGCACCCCCCCCCCCACGGGACTCCCCTTCCCGGGCCGGAGGGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                       <  1:371794/134‑1 (MQ=255)
                                                                                                            acAACTCACCCCACCTCGCCCGCAAACCCCCCCCCCAAGGCAATCCCCTCCCCCTGCCGGTGGGGGCGCGCCCGCTACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                       <  5:93436/134‑1 (MQ=255)
                                                                                                                   aCAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCCCCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATCGCCCCAGCCTCAAGCTCCATGTGGTACGCAACAAGCAAGCATCGAGCGCGCAgg                >  3:304183/1‑135 (MQ=255)
                                                                                                                   aCAGCACCGCGCCAGGCAACCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGc               <  7:242775/136‑1 (MQ=255)
                                                                                                                    ccccaccacGCCAGGCACCCCGCCCCCCACGGGAAACACCTACCCCTGCGGGAGGGGGCTGGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGc               <  2:397408/132‑1 (MQ=255)
                                                                                                                    cAGCACCGCGCCCGGCACCCCGCCCCCCACGGGAATCACCTTCCCCTGCCGGCGCGGGCTGGCCCGCGACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGc               <  1:289167/135‑1 (MQ=255)
                                                                                                                        aCCTCGCCCGCCAACCCGCCCCCAAAGGCAATCACCTTCCCCTGCAGCAGCGGGATTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGa           <  3:161795/135‑1 (MQ=255)
                                                                                                                           tCGCCAGGCAACCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGc        <  6:232079/135‑1 (MQ=255)
                                                                                                                           tCGCCAGCCACCCCCCCCCCCAAGGGAATCACCTCCCCCGGCAGGCGCGGGCTTGCCCGCGATGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGc        <  4:363712/135‑1 (MQ=255)
                                                                                                                             gCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCAGGAAGCCt      >  1:235520/1‑135 (MQ=255)
                                                                                                                                aGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCg  >  7:267415/1‑136 (MQ=255)
                                                                                                                                |                                                                                                                                       
TTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCG  >  KT2440/4874439‑4874702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑