Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,874,607 A→C 34.0% intergenic (+53/‑134) CJ019_04568 → / → uacT_1 hypothetical protein/Uric acid transporter UacT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,874,6070AC34.0% 43.7 / 2.9 45intergenic (+53/‑134)CJ019_04568/uacT_1hypothetical protein/Uric acid transporter UacT
Reads supporting (aligned to +/- strand):  ref base A (17/10);  new base C (7/8);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 3.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGTGCTGCA  >  KT2440/4874481‑4874741
                                                                                                                              |                                                                                                                                      
aCATCCCCCAGATCCCCCCCGAAGAGCGACCGCGGGCGGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCtt                                                                                                                                <  6:957/135‑1 (MQ=255)
    cACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGCATCCCCTTCCCCGGCAGGAGCGGGCTTGccc                                                                                                                            >  3:337563/1‑135 (MQ=255)
         aGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCCGCAACCCGCCCCCCAAGGGCATCACCATCCCCGGCAGGCGCGGGCGTGCCcgc                                                                                                                          >  1:136951/1‑132 (MQ=255)
            tGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAACTCACAGCACCTCGCCCGGCAACCCCCCCCCCAACGGAATCACCATCCCCTGCAGGCGCCGGCTTGCCCCCCAagag                                                                                                                    >  2:250168/1‑135 (MQ=255)
              accaccGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCCAGGGAATCACCTTCCCCTGCAGGAGCGGGCGTGGCCGCGCAGAGGc                                                                                                                  >  3:58561/1‑135 (MQ=255)
                 accGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTGAAATGCCAGCACCGCGCCACGCAACCCCCCCCCCCAGGGAATGCCCATCCCCAGCAGGCGCCGGCGTTCCCGCGCAGAGGCCg                                                                                                                >  3:210285/1‑134 (MQ=255)
                   cGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCCAAGGCGCCCCGGTCAACAGACAGCCCCACCCCACGCCACCCCCCCCCCCACGGAATAACCATCCCCAGCAGGCGCCGGCATGCCCGCCGAGAGGCCGaaa                                                                                                             >  2:195249/1‑133 (MQ=255)
                        agCGCAAGCTCGTCGGCGACTGGATCGCCCAAGGCGCCCCGGTCAACTGACAGCCCCACGCCCCGCCACCCCCCCCCCACCGGCATCACCATCCCCAGCAGGGGCGGGCGTGCCCGCGAAGAGGCCGCAAGcccc                                                                                                        >  4:265495/1‑135 (MQ=255)
                        agCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCACGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCATCCCCAGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGcccc                                                                                                        >  6:162208/1‑135 (MQ=255)
                            cAAGCGCGGCGGCGACTGGATCGCCAACGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCACCCCCCCCCCCAAGGGAATCACCTTCCCCTGCCGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGcc                                                                                                    <  4:337563/135‑1 (MQ=255)
                               gCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCAGCAGGCGCGGGCTTGCCCGCGCAGAGGCCGCTAGCCCCAGCCTcc                                                                                                 >  5:197621/1‑134 (MQ=255)
                                       gCGACTTGATCGCCAAAGGCGCCCAGGGCAAATGCCAGCACCTCGCCCGGCCACCCCCCCCCCAACGGAATCACCGTCCCCTGCAGGCGCGGGGTTGCCCGCGCAGCGGCCGATAGCCCCCGCCGCCAGCGCCaa                                                                                         >  4:216422/1‑134 (MQ=255)
                                       gCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCCCCGTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGCGGCCGCTAGCCCCAGCCGCACGCTccct                                                                                         >  4:295187/1‑133 (MQ=255)
                                       gCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCCACCCGCCCCCCAAGGGAATCACCATCCCCTGCAGGAGCGGGCTTGCCCCCGAAGAGGCCGATAGCCCCAGCCTCCAGCTCCAtg                                                                                        >  7:34650/1‑136 (MQ=255)
                                          tcTGGAGCGCAAACGGCGCCCTGGTCAACTGACACCACCTCGCCAGGCACCCCGCCCCCCACGGTAATCCCCTTCCCCTGCTGGCGGGGGGTGGCCCGCTACGAGGCCGATAGCCCCAGCCTCAAGCTCCAtgtg                                                                                      <  2:136951/134‑1 (MQ=255)
                                              gATCCCCAAAGGCGCCCAGGTCCACAGACACCACCTCCCCACGCAACCCCCCCCCCAACGGAATCACCATCCCCTGCAGGCGCGGGCTTGCCCGCGAAGACGCCCATAGCCCCCCCCACAAGCTCCATCTGtttc                                                                                  >  4:215281/1‑133 (MQ=255)
                                               agccccAACGCCGCCCAGGTCAACTGACAGCACCCCCCCCGCCACCCCCCCCCCCAAGGCAATCACCTCCCCCTCCAGGCGCGGGCTGGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                                                                 <  6:447056/131‑1 (MQ=255)
                                               aTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCACCCCGCCCCCCAAGGTAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                                                                 <  1:31617/135‑1 (MQ=255)
                                               aTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGCGGGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                                                                 <  1:14424/135‑1 (MQ=255)
                                                   ccAACGGCGCCCAGGTCAACTGACACCACCTCGCCAGCCAACCCCCCCCCCAAGGGAATCACCTTCCCCTGCAGGCGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac                                                                             <  6:99747/135‑1 (MQ=255)
                                                   caaaaGGCGCCCCGGTCAACGTACACCACCTCGCCCGGCAACCCGCCCCCCATGTAAATCCCCTACCCGGGCTGGCGCGGGCTTGCCGGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac                                                                             <  3:324425/133‑1 (MQ=255)
                                                                 gacAACTGACAGCACCGCGCCCGGCACCCCGCCCCCCACGGGAACCACCACCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                                                              <  7:11381/134‑1 (MQ=38)
                                                                  tCAACTCACAGCACCTCGCCAGGAAACCCGCCCCCCAGCGTAATCACCTTCCCGTGCAGGAGCGTGCTTGCCGGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                                                              <  3:421705/135‑1 (MQ=255)
                                                                  acAACTGACACCACCGCGCCCGGCACCCCCCCCCCCACGGGACTCCCCTTCCCGGGCCGGAGGGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                                                              <  1:371794/134‑1 (MQ=255)
                                                                  acAACTCACCCCACCTCGCCCGCAAACCCCCCCCCCAAGGCAATCCCCTCCCCCTGCCGGTGGGGGCGCGCCCGCTACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                                                              <  5:93436/134‑1 (MQ=255)
                                                                         aCAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCCCCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATCGCCCCAGCCTCAAGCTCCATGTGGTACGCAACAAGCAAGCATCGAGCGCGCAgg                                                       >  3:304183/1‑135 (MQ=255)
                                                                         aCAGCACCGCGCCAGGCAACCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGc                                                      <  7:242775/136‑1 (MQ=255)
                                                                          ccccaccacGCCAGGCACCCCGCCCCCCACGGGAAACACCTACCCCTGCGGGAGGGGGCTGGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGc                                                      <  2:397408/132‑1 (MQ=255)
                                                                          cAGCACCGCGCCCGGCACCCCGCCCCCCACGGGAATCACCTTCCCCTGCCGGCGCGGGCTGGCCCGCGACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGc                                                      <  1:289167/135‑1 (MQ=255)
                                                                              aCCTCGCCCGCCAACCCGCCCCCAAAGGCAATCACCTTCCCCTGCAGCAGCGGGATTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGa                                                  <  3:161795/135‑1 (MQ=255)
                                                                                 tCGCCAGGCAACCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGc                                               <  6:232079/135‑1 (MQ=255)
                                                                                 tCGCCAGCCACCCCCCCCCCCAAGGGAATCACCTCCCCCGGCAGGCGCGGGCTTGCCCGCGATGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGc                                               <  4:363712/135‑1 (MQ=255)
                                                                                   gCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCAGGAAGCCt                                             >  1:235520/1‑135 (MQ=255)
                                                                                      aGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCg                                         >  7:267415/1‑136 (MQ=255)
                                                                                               gCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCGTGGATc                                 >  3:396171/1‑135 (MQ=255)
                                                                                                                    ccccTGCCGGGGGGGGCTTGCCCGCTACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGACGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCg            <  7:120583/135‑1 (MQ=255)
                                                                                                                    ccccTCCCGGCGGGGGCTGCCCCGCAAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCg            <  5:146492/135‑1 (MQ=255)
                                                                                                                      ccTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTTCGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTccat          >  1:347456/1‑132 (MQ=255)
                                                                                                                      ccTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGACGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAgg         >  8:257824/1‑136 (MQ=255)
                                                                                                                          cAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGtgc      >  5:213237/1‑135 (MQ=255)
                                                                                                                             gAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGtgctgc   >  3:414474/1‑135 (MQ=255)
                                                                                                                             gAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGtgctgc   >  4:443911/1‑135 (MQ=255)
                                                                                                                             gAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGTGCTGCa  >  8:62895/1‑136 (MQ=255)
                                                                                                                             gAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAAGAAAAACAAAACTCGAGGGGCTGCa  >  7:327223/1‑136 (MQ=255)
                                                                                                                             gAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGAATCGAGCGCGCACGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGtgctgc   >  6:414267/1‑135 (MQ=255)
                                                                                                                              |                                                                                                                                      
ACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGTGCTGCA  >  KT2440/4874481‑4874741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: