Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | KT2440 | 4,874,607 | A→C | 34.0% | intergenic (+53/‑134) | CJ019_04568 → / → uacT_1 | hypothetical protein/Uric acid transporter UacT |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | KT2440 | 4,874,607 | 0 | A | C | 34.0% | 43.7 / 2.9 | 45 | intergenic (+53/‑134) | CJ019_04568/uacT_1 | hypothetical protein/Uric acid transporter UacT |
Reads supporting (aligned to +/- strand): ref base A (17/10); new base C (7/8); total (25/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.47e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGTGCTGCA > KT2440/4874481‑4874741 | aCATCCCCCAGATCCCCCCCGAAGAGCGACCGCGGGCGGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCtt < 6:957/135‑1 (MQ=255) cACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGCATCCCCTTCCCCGGCAGGAGCGGGCTTGccc > 3:337563/1‑135 (MQ=255) aGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCCGCAACCCGCCCCCCAAGGGCATCACCATCCCCGGCAGGCGCGGGCGTGCCcgc > 1:136951/1‑132 (MQ=255) tGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAACTCACAGCACCTCGCCCGGCAACCCCCCCCCCAACGGAATCACCATCCCCTGCAGGCGCCGGCTTGCCCCCCAagag > 2:250168/1‑135 (MQ=255) accaccGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCCAGGGAATCACCTTCCCCTGCAGGAGCGGGCGTGGCCGCGCAGAGGc > 3:58561/1‑135 (MQ=255) accGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTGAAATGCCAGCACCGCGCCACGCAACCCCCCCCCCCAGGGAATGCCCATCCCCAGCAGGCGCCGGCGTTCCCGCGCAGAGGCCg > 3:210285/1‑134 (MQ=255) cGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCCAAGGCGCCCCGGTCAACAGACAGCCCCACCCCACGCCACCCCCCCCCCCACGGAATAACCATCCCCAGCAGGCGCCGGCATGCCCGCCGAGAGGCCGaaa > 2:195249/1‑133 (MQ=255) agCGCAAGCTCGTCGGCGACTGGATCGCCCAAGGCGCCCCGGTCAACTGACAGCCCCACGCCCCGCCACCCCCCCCCCACCGGCATCACCATCCCCAGCAGGGGCGGGCGTGCCCGCGAAGAGGCCGCAAGcccc > 4:265495/1‑135 (MQ=255) agCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCACGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCATCCCCAGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGcccc > 6:162208/1‑135 (MQ=255) cAAGCGCGGCGGCGACTGGATCGCCAACGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCACCCCCCCCCCCAAGGGAATCACCTTCCCCTGCCGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGcc < 4:337563/135‑1 (MQ=255) gCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCAGCAGGCGCGGGCTTGCCCGCGCAGAGGCCGCTAGCCCCAGCCTcc > 5:197621/1‑134 (MQ=255) gCGACTTGATCGCCAAAGGCGCCCAGGGCAAATGCCAGCACCTCGCCCGGCCACCCCCCCCCCAACGGAATCACCGTCCCCTGCAGGCGCGGGGTTGCCCGCGCAGCGGCCGATAGCCCCCGCCGCCAGCGCCaa > 4:216422/1‑134 (MQ=255) gCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCCCCGTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGCGGCCGCTAGCCCCAGCCGCACGCTccct > 4:295187/1‑133 (MQ=255) gCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCCACCCGCCCCCCAAGGGAATCACCATCCCCTGCAGGAGCGGGCTTGCCCCCGAAGAGGCCGATAGCCCCAGCCTCCAGCTCCAtg > 7:34650/1‑136 (MQ=255) tcTGGAGCGCAAACGGCGCCCTGGTCAACTGACACCACCTCGCCAGGCACCCCGCCCCCCACGGTAATCCCCTTCCCCTGCTGGCGGGGGGTGGCCCGCTACGAGGCCGATAGCCCCAGCCTCAAGCTCCAtgtg < 2:136951/134‑1 (MQ=255) gATCCCCAAAGGCGCCCAGGTCCACAGACACCACCTCCCCACGCAACCCCCCCCCCAACGGAATCACCATCCCCTGCAGGCGCGGGCTTGCCCGCGAAGACGCCCATAGCCCCCCCCACAAGCTCCATCTGtttc > 4:215281/1‑133 (MQ=255) agccccAACGCCGCCCAGGTCAACTGACAGCACCCCCCCCGCCACCCCCCCCCCCAAGGCAATCACCTCCCCCTCCAGGCGCGGGCTGGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg < 6:447056/131‑1 (MQ=255) aTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCACCCCGCCCCCCAAGGTAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg < 1:31617/135‑1 (MQ=255) aTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGCGGGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg < 1:14424/135‑1 (MQ=255) ccAACGGCGCCCAGGTCAACTGACACCACCTCGCCAGCCAACCCCCCCCCCAAGGGAATCACCTTCCCCTGCAGGCGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac < 6:99747/135‑1 (MQ=255) caaaaGGCGCCCCGGTCAACGTACACCACCTCGCCCGGCAACCCGCCCCCCATGTAAATCCCCTACCCGGGCTGGCGCGGGCTTGCCGGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac < 3:324425/133‑1 (MQ=255) gacAACTGACAGCACCGCGCCCGGCACCCCGCCCCCCACGGGAACCACCACCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 7:11381/134‑1 (MQ=38) tCAACTCACAGCACCTCGCCAGGAAACCCGCCCCCCAGCGTAATCACCTTCCCGTGCAGGAGCGTGCTTGCCGGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 3:421705/135‑1 (MQ=255) acAACTGACACCACCGCGCCCGGCACCCCCCCCCCCACGGGACTCCCCTTCCCGGGCCGGAGGGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 1:371794/134‑1 (MQ=255) acAACTCACCCCACCTCGCCCGCAAACCCCCCCCCCAAGGCAATCCCCTCCCCCTGCCGGTGGGGGCGCGCCCGCTACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 5:93436/134‑1 (MQ=255) aCAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCCCCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATCGCCCCAGCCTCAAGCTCCATGTGGTACGCAACAAGCAAGCATCGAGCGCGCAgg > 3:304183/1‑135 (MQ=255) aCAGCACCGCGCCAGGCAACCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGc < 7:242775/136‑1 (MQ=255) ccccaccacGCCAGGCACCCCGCCCCCCACGGGAAACACCTACCCCTGCGGGAGGGGGCTGGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGc < 2:397408/132‑1 (MQ=255) cAGCACCGCGCCCGGCACCCCGCCCCCCACGGGAATCACCTTCCCCTGCCGGCGCGGGCTGGCCCGCGACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGc < 1:289167/135‑1 (MQ=255) aCCTCGCCCGCCAACCCGCCCCCAAAGGCAATCACCTTCCCCTGCAGCAGCGGGATTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGa < 3:161795/135‑1 (MQ=255) tCGCCAGGCAACCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGc < 6:232079/135‑1 (MQ=255) tCGCCAGCCACCCCCCCCCCCAAGGGAATCACCTCCCCCGGCAGGCGCGGGCTTGCCCGCGATGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGc < 4:363712/135‑1 (MQ=255) gCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCAGGAAGCCt > 1:235520/1‑135 (MQ=255) aGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCg > 7:267415/1‑136 (MQ=255) gCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCGTGGATc > 3:396171/1‑135 (MQ=255) ccccTGCCGGGGGGGGCTTGCCCGCTACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGACGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCg < 7:120583/135‑1 (MQ=255) ccccTCCCGGCGGGGGCTGCCCCGCAAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCg < 5:146492/135‑1 (MQ=255) ccTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTTCGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTccat > 1:347456/1‑132 (MQ=255) ccTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGACGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAgg > 8:257824/1‑136 (MQ=255) cAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGtgc > 5:213237/1‑135 (MQ=255) gAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGtgctgc > 3:414474/1‑135 (MQ=255) gAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGtgctgc > 4:443911/1‑135 (MQ=255) gAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGTGCTGCa > 8:62895/1‑136 (MQ=255) gAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAAGAAAAACAAAACTCGAGGGGCTGCa > 7:327223/1‑136 (MQ=255) gAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGAATCGAGCGCGCACGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGtgctgc > 6:414267/1‑135 (MQ=255) | ACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCTGCCGCTTGGATCCGAGAATAAAAACAAAACTCGAGGTGCTGCA > KT2440/4874481‑4874741 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |