| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,927,692 | A→C | 53.5% | W194G (TGG→GGG) | srp54 ← | Signal recognition particle 54 kDa protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,927,692 | 0 | A | C | 53.5% | ‑6.2 / 5.5 | 24 | W194G (TGG→GGG) | srp54 | Signal recognition particle 54 kDa protein |
| Reads supporting (aligned to +/- strand): ref base A (7/3); new base C (10/3); total (17/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
GGCCAGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCG‑ATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGC‑GCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCA > KT2440/4927561‑4927814 | ggCCAGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCG‑ATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGc > 2:146293/1‑135 (MQ=255) ccAGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCG‑ATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCt > 2:185724/1‑135 (MQ=255) aGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCG‑ATCACCCCCCCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCAGGGCGCGGctct > 5:59101/1‑135 (MQ=255) ggtggtCTTGCCCATGCCCGCCGGGCCGACCATGGCG‑ATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCTGCgct > 3:353467/1‑133 (MQ=255) ccggtCTGCCCCAGCCCCGCCGGCCCGACCAGGGCG‑ATCACCCCCCCCTCTGCGATCGGCTCTATTCCCGCCACTTCTATCATCCGTGCCAAGTGACCCACCCCCATGCGCCCGGCCGGGCGCGGCTCTTCGAtct < 8:119726/134‑1 (MQ=255) ggctcgCCCTTGCCGGCCGGCCCGCCCATGCCG‑ATCACCCCCCCGTTTTCTATCGTCTAGTTTTCAGGCTCTTCGATCATCCGTGCCAAGTGAGCCAGCCGCCTGCGCCTGCCGTGCCGCGGCTCTTCGATCTcgg < 8:275471/131‑1 (MQ=255) cccATGCCCGCCGGGCCGCCCATGGCG‑ATCACCCCACCCTCTTCGATCGGCTCGATTTCATGCACTTCGCGCATCCGTGCCAACTGAGCCAGCAGCATGCGCCAGGCCGGGCGCGGCACGTCGATGGCGGCGGt > 7:443542/1‑134 (MQ=255) cccATGCCCGCCGGGCCGACCATGGCG‑ATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGGGCTTCGCTCTcggcgg > 2:66287/1‑133 (MQ=255) cATGCCCGCCGGGCCGACCATGGCG‑ATCACCCCCCCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCGGGCGCGGCGCGTCGAGCGcggcggggagg > 7:69857/1‑131 (MQ=255) gCCCGCCGGCCCGCCCA‑GGCGCAGCCCCCCCCCCTCTCCGAGCGGCGCGATTGCCGGCACTGCGATCATCCGCGCAATGTGCCCCAGCCGCTGGCGCCAGCCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTc < 2:364935/135‑1 (MQ=255) cgccgGGCCGACCATGGCG‑ATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGGGCTTCGTTCTCGGCGGGCCGGTGcag > 6:96897/1‑135 (MQ=255) aCCATGGCG‑ATCACCCCACCCTCTTCGCTCGCCTCGATTTCAGCCACTTCGACCATCCGTGCCAAGTGCGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg < 8:312599/136‑1 (MQ=255) cccacccTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCCGCCAGGCCCGGCGCTAGGATCCCGGCGGTGAAGTACACCAGCTCCCGGGGGAAGGGGCCGGAc > 7:56434/1‑136 (MQ=255) ccTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCAGGCGCGGCGCTTTGCTGTGGGCGGTCAGGTCCACCAGCGCGCGGGCGCTAGGGCCGCGCAGGc > 3:137416/1‑135 (MQ=255) gATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCGCTTCGCTCTCGGCGGTCAGGGCCAGCCGCTCGCGGGCGATCGGCCCCGCCCGGCCCCTgcg > 4:52892/1‑135 (MQ=255) gATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGGTTGGTACTCGGGGGTGAGGTACCGCAGCTCGCGGGCGAGAGGCCCCGACACGCCgctcggg > 8:29330/1‑130 (MQ=255) aTCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCGGGCGCGGGGCTTCGATCTCGGCGGTCAGGTCCAGCCGCGAGCCGGCGCAAGGCCCGGACACGCCGATgcgcg > 8:124825/1‑135 (MQ=255) cGATTTCAGGCACTTCGCTCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCACGCCTGGCGCCGCGCTTCGATCAAGGCGGTCAGGTCCAGCCGCTCGCGGGCGCTCTGCCCGGACCGGCCGATGCGCTgacggc > 2:308630/1‑130 (MQ=255) cGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAggc > 4:295899/1‑135 (MQ=255) tttCAGGCCCTGCGATCACCCGCCCCACGGGACCCCCCCCCCGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAggcggc < 4:4131/135‑1 (MQ=255) tcatcCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCGCGTGGATCTCGGCGGGGAGGGGCAGCAGCTCGCGGGCGATCGGCCCCGCCAGGCCCCTGCGCGGCCGGCGGCGGCCAACGTTTGGcg > 4:387161/1‑134 (MQ=255) cAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCGCGTGGATCTCGGGGGGCAGGGCCCCCACCACGCGGGCGGTCGGCCCCGCCCCGCCCCTGCGCAGCCGGCGGC‑GCCCAAGGTTTGCCAGCGGCCGcgt > 6:170711/1‑133 (MQ=255) gAGCCAGCAGCCGGCGCCAGCCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAGCGGCCCGGACAGGCCGATGCGCTGCAGGCGGC‑GCCAAAGGTTGGCCTGCTGCGGCTtgctgc < 6:84456/135‑1 (MQ=255) cccgcaGGCGCCCGGCCGGCCGCGGCTCTTCGATCTCGGCGGGCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGC‑GCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCa < 2:71973/132‑1 (MQ=255) | GGCCAGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCG‑ATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGC‑GCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCA > KT2440/4927561‑4927814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |