Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	5,099,788	T→G	23.0%	H717P (CAC→CCC)	CJ019_04795 ←	putative protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,099,788	0	T	G	23.0%	67.2 / 3.6	55	H717P (CAC→CCC)	CJ019_04795	putative protein
Reads supporting (aligned to +/- strand):  ref base T (21/20);  new base G (12/2);  total (33/22)
Fisher's exact test for biased strand distribution p-value = 2.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAACGCGACAACGGGTCAGGGTAGCCTGGAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATCCGGCACATTGACCTTGATCGGGCACTGCC  >  KT2440/5099653‑5099920                                                                                                                                        |                                                                                                                                     cgaaCGCGACAACGGGTCAGGGTAGCCAGGATGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCACAGCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGt                                                                                                                                      >  7:396066/3‑136 (MQ=255) ccAACGCGACAACGGGTCAGGGTAGCCTGGAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGt                                                                                                                                      <  8:337244/136‑1 (MQ=255)    aCGCGACAACGGGTCAGGGTAGCCTGGAAGTGGAAGCGGCTGATCAGCGGGCTGACGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTgc                                                                                                                                    <  2:129969/135‑1 (MQ=255)       cgACAACGGGTCAGGGTAGCCTGGAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCa                                                                                                                                <  7:247320/136‑1 (MQ=255)        gACAACGGGTCAGGGTAGCCTGGAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCa                                                                                                                                <  6:186985/135‑1 (MQ=255)        gACAACGGGTCAGGGTAGCCTGGAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGGGCGCCAg                                                                                                                               >  7:259811/1‑136 (MQ=255)        gACAACGGGTCAGGGTAGCCTGGAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCGTGTCGGCCAGCAGCAGGCTCCCCCACTTCGACCCCCTCACCTCGTTCTACAGCCCCGGCGCGGGCGCCAg                                                                                                                               >  8:383654/1‑136 (MQ=255)                  cAGGGTAGCCTGGAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGAt                                                                                                                      <  3:423636/135‑1 (MQ=255)                   aGGGTAGCCTGGAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATg                                                                                                                     >  3:341160/1‑135 (MQ=255)                             ggAAGTTGAAGCGGCTGATCAGCGGGCTGTCGCCCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTGCGAGCCCAGCACCGCGTTGTACAGCCCCGGCGCGTGCTCCAGGGAGGGGAGGGTGCAcgggag                                                                                                          >  7:386700/1‑131 (MQ=255)                             ggAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCTGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGAGTGCGCCAGGTAGGGGATGGTGAACTcgag                                                                                                          <  8:445004/136‑1 (MQ=255)                             ggAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTcgag                                                                                                          >  7:254870/1‑136 (MQ=255)                             ggAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGGAGGGGATGGTGAACTcga                                                                                                           >  2:369641/1‑135 (MQ=255)                             ggAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGGGCGCCAGGTAGGGGATGGTGAACTcgc                                                                                                           >  2:307303/1‑134 (MQ=255)                             ggAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGGTGTACAGCCCCGGCGCGGGCGCCAGGGAGGGGATGGTGAACTcgag                                                                                                          >  8:364987/1‑136 (MQ=255)                             ggAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTAGAGCCCATCCCCGCGTTGTACAGCCCCGCCGCCGGCGCCAGGTTGGGGCTTGTGCAcaagag                                                                                                          >  8:427399/1‑131 (MQ=255)                                  ttGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGGGGCAGGTTGGGGTTGGGGGAATcgagcgcg                                                                                                      >  6:250080/1‑133 (MQ=255)                                  ttGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGGGGGCCAGGGAGGGGGTGGTGGACTcgagcgag                                                                                                      >  6:43489/1‑135 (MQ=255)                                  ttGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGGGCGCCAGGTAGGGGATGGTGAACTcgagcgag                                                                                                      >  2:113325/1‑135 (MQ=255)                                  ttGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGGGCGCCAGGGAGGGGATGGTGAACTcgagcgag                                                                                                      >  4:261969/1‑135 (MQ=255)                                       gCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGGGCGCCAGGGTGGGGGTGGGGAACGCGCGCGCGCCgcg                                                                                                 >  1:143163/1‑133 (MQ=255)                                                cAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGGAGGGGATGGGGAACTCGAGCGCGCCGATCAGGTAGTc                                                                                        >  2:105225/1‑135 (MQ=255)                                                  gCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACg                                                                                     <  8:395290/136‑1 (MQ=255)                                                     ggCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCa                                                                                   <  5:43489/135‑1 (MQ=255)                                                        tGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGATCTCGAGCGAGCCGATCAGGGAGTGCCgcggcg                                                                                >  2:154771/1‑135 (MQ=255)                                                           cGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCgg                                                                               <  1:113325/133‑1 (MQ=255)                                                           cGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCgg                                                                               <  5:144875/133‑1 (MQ=255)                                                                   cccACGTTGGCGGCCAGCCGCTGGCCCACCAACTTCCCCCCAATCCCCGCGTTGTCCACCCCCGGCGCGTGCGCCAGGTCGGGGATGGTGACCTGGAGCGCCCCCATCAGGTAGTCACGCAGCGGGCGCTGGTAg                                                                     <  6:25606/135‑1 (MQ=255)                                                                    ccACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCTGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCg                                                                   <  7:206884/136‑1 (MQ=255)                                                                     cACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCCATCAGGTAGTCACGCAGCGGGCGCTGGTAGCg                                                                   >  6:127971/1‑135 (MQ=255)                                                                     cACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGGAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCGGGTAGCg                                                                   >  5:149967/1‑135 (MQ=255)                                                                     cACCTTGTCGGCCAGCAGCTGGCTCACCAACTGCGAGCCCATCCCCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTTGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCg                                                                   <  2:390247/135‑1 (MQ=255)                                                                           gTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCCCCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTg                                                             <  2:89769/135‑1 (MQ=255)                                                                           gTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGCGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTg                                                             >  2:277696/1‑135 (MQ=255)                                                                               gCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGGGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTTGTCACGCAGCCGGCGCTGGTAGCGACCggggg                                                           >  4:20151/1‑132 (MQ=255)                                                                               gCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTGGTACAGCCCCGGCGCGTGCGCCAGGGAGGGGCTGGTGCACGCGAGCGCGCCGATCCGGTAGTCCCGCAGCGGGCGCGGGGGGCGCCCGGGGTa                                                          >  7:14567/1‑134 (MQ=255)                                                                                     agcTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGGGGGCCCGGGATGGGAGTGGGGACTGGAGCGGGCCGCGCCGGGAGTCACCCCGCGGGCGCTGGGAGCGGCCGGGGGAAAGcgcg                                                   >  2:441316/1‑135 (MQ=255)                                                                                                        aGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATc                               <  8:278688/136‑1 (MQ=255)                                                                                                         gCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATc                               <  6:159034/135‑1 (MQ=255)                                                                                                                 ccGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGCTCAGGTAGTCACGCAGCGGGCGCTGGTAGCGCCCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATCCGGCAAAtt                      >  7:49040/1‑136 (MQ=255)                                                                                                                  cgcgTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATCCGGCACAtt                      <  6:443200/135‑1 (MQ=255)                                                                                                                   gctttGTACAGCCCCGGCGCGTGCGGCTGGTAGTGTATGGTGAAGTCGAGCGAGCCGATCAGGTAGTCACGCAGCGTGCGATTGTAGCGACCGTGGTTAAGATCGAGTAATCTCGAGCTGAAATCCGGCACATTGa                    <  8:396066/133‑1 (MQ=255)                                                                                                                   gcgTTGTACAGCCCCGGCGCGGGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATCCGGCACATTGa                    <  7:424524/136‑1 (MQ=255)                                                                                                                       tGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATCCGGCACATTGACCt                 >  1:301656/1‑135 (MQ=255)                                                                                                                       tGTACAGCCCCGGCGCGGGCGGCAGGTAGGGGATGGTGAGCTCGCGCGCGCCGATCAGGTAGTGACGCCGCGGGCGCTGGTTGGGCCCGTGGTTAAGCGCGAGGGAGCGCGAGCGGAAATGCGGCACATTGACCt                 >  1:269088/1‑135 (MQ=255)                                                                                                                       ccccaggCCCCGGCGCGGGCGCCCGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATCCGGCACATTGACCtt                <  7:176405/130‑1 (MQ=255)                                                                                                                            aGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATCCGGCACATTGACCTTGATc            <  1:19268/135‑1 (MQ=255)                                                                                                                             gCCCCGGCGCGTGCGCCTGGTAGGGGATGGGGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATCCGGCACATTGACCTTGATCgg          <  8:259811/136‑1 (MQ=255)                                                                                                                                 cGGCGCGTGCGCCAGGTATGGGATGGTGAACTCGAGCGAGCCGAGCAGGTAGTCCCGCAGCGGGCGCGGGGTGCGACCGTGGTAAAGCTCGCGGAAGCGCGCGCGGAAATCCGGCACATTGCCCTTGCTCGGGcc       >  4:159245/1‑134 (MQ=255)                                                                                                                                 cGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGCGCCGATCAGGTTGTAACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATCCGGCACATTGACCTTGATAGGGCa       >  8:266238/1‑135 (MQ=255)                                                                                                                                 cGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGCGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTg                                                             <  3:97621/81‑1 (MQ=255)                                                                                                                                 cGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTg                                                             >  4:97621/1‑81 (MQ=255)                                                                                                                                    cgcgTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATCCGGCACATTGACCTTGATCGGGCACTg    >  2:308456/1‑135 (MQ=255)                                                                                                                                    cgcgTGCGCCAGGTAGGGGATGGTGAACTAGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGCTAGCGACCGTGGTAAAGATCGAGGAAGCGCGAGCGGAAATCCGGCCCATTGACCTTGATCGGGCACTg    >  2:389687/1‑135 (MQ=255)                                                                                                                                      cgTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGCGCGGAAATCCGGCACATTGACCTTGATCGGGCACTGcc  >  3:76013/1‑135 (MQ=255)                                                                                                                                      cgTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGCGCGGAAATCCGGCACATTGACCGTGATCGGGCACTGcc  >  6:228494/1‑135 (MQ=255)                                                                                                                                      cgTGCGCCACGTAGGGGATGGTGAACTAGATCGAGCCCCTCAGGTAGTCACGCAGCGGGCGCTGGTAGCGCCCGTGGTAAAGCACGAGGAAGCGCGCGCGGAAATCCGGCACATTGACCATGATAGGGCACTGcc  >  4:216373/1‑135 (MQ=255)                                                                                                                                        |                                                                                                                                     CCAACGCGACAACGGGTCAGGGTAGCCTGGAAGTTGAAGCGGCTGATCAGCGGGCTGTCGACCATGCCCACCTTGTCGGCCAGCAGCTGGCTCACCCACTTCGAGCCCATCACCGCGTTGTACAGCCCCGGCGCGTGCGCCAGGTAGGGGATGGTGAACTCGAGCGAGCCGATCAGGTAGTCACGCAGCGGGCGCTGGTAGCGACCGTGGTAAAGCTCGAGGAAGCGCGAGCGGAAATCCGGCACATTGACCTTGATCGGGCACTGCC  >  KT2440/5099653‑5099920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑