Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,101,424 T→G 11.3% T172P (ACC→CCC)  CJ019_04795 ← putative protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,101,4240TG11.3% 196.4 / 4.7 84T172P (ACC→CCC) CJ019_04795putative protein
Reads supporting (aligned to +/- strand):  ref base T (25/47);  new base G (7/5);  total (32/52)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGAGCGCAGCATTGAGCTGGTCCT  >  KT2440/5101293‑5101558
                                                                                                                                   |                                                                                                                                       
caggCGGTACACCGCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCCGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGtttt                                                                                                                                      <  5:153659/132‑1 (MQ=255)
    cGGTACACCTCGCCGCACCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGTTCGGCAGGCGGTGCAGGCGTTCGCCAACGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGGTTTACCGt                                                                                                                                 <  8:94922/136‑1 (MQ=255)
    cGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGt                                                                                                                                 <  8:9874/136‑1 (MQ=255)
     ggTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGt                                                                                                                                 <  1:363985/135‑1 (MQ=255)
          acCTCGCCGACGCGGCCCTGCGCGGCGCAGGCCTGTTCCAGCGCAGCATGGTCGATCGGCAGGCTGTGCATGCGTTCGCCTCCGTGCAGCACTCTTTGCAGCTCCAGCACTTGGTCGCGGTTTTTACCGTAGGTg                                                                                                                            <  5:156016/135‑1 (MQ=255)
          acCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTg                                                                                                                            <  6:289692/135‑1 (MQ=255)
               gCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCATCACGTGGTCGCGGGGTTTAACGGAGGTGCAGCt                                                                                                                       <  1:216914/135‑1 (MQ=255)
               gCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCt                                                                                                                       >  1:349596/1‑135 (MQ=255)
               gCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCt                                                                                                                       >  2:286074/1‑135 (MQ=255)
                ccGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCt                                                                                                                       <  6:65851/134‑1 (MQ=255)
                ccGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCt                                                                                                                       <  5:363291/134‑1 (MQ=255)
                 cGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGGTTTACCGTAGGTGCAGCGGc                                                                                                                     >  5:450191/1‑135 (MQ=255)
                  gACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGAGCCGCATCGTCGAGCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCCGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGGTTTAACGTAGGGGCCGCGGcc                                                                                                                    >  4:5525/1‑135 (MQ=255)
                   aCCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCt                                                                                                                  <  7:310950/136‑1 (MQ=255)
                        gcccggcGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTGCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGcc                                                                                                              <  3:19345/135‑1 (MQ=255)
                        gcccggcGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGcc                                                                                                              <  5:266144/135‑1 (MQ=255)
                            ggcgcggcgcAGGCCTGTTCCAGCGCAGCATCGTCGCTCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTAGCGTAGGGGGAGCGGGCCTGGGCAcgg                                                                                                          >  3:157513/1‑133 (MQ=255)
                            ggcgcggcgcAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTgg                                                                                                         <  7:242131/136‑1 (MQ=255)
                            ggcgcggcgcAGGCCTGTTCCAGCGCAGCATCGTCGAGCGGCAGGCTGTGCAGGCGTTCGCCCCCGAGCCGCACGCTGTGCAGCGCCCGCACGTGGTCGCGGGTGTTACCGGAGGTGCCGCTGCCCTGGccccgg                                                                                                          >  6:284929/1‑131 (MQ=255)
                             gcgcggcgcAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTgg                                                                                                         <  2:411689/135‑1 (MQ=255)
                             gcgcggcgcAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTgg                                                                                                         <  6:143598/135‑1 (MQ=255)
                             gcgcggcgcAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTgg                                                                                                         <  6:35980/135‑1 (MQ=255)
                             gcgcggcgcAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTgg                                                                                                         <  6:450191/135‑1 (MQ=255)
                             gcgcggcgcAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTgg                                                                                                         <  4:372349/135‑1 (MQ=255)
                                cggcgcAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGCGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGGTTTTCCGTTGGTGGAGCTGCCCGGGCCCCTGGcct                                                                                                      >  6:266144/1‑133 (MQ=255)
                                         ccTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGa                                                                                            <  2:277991/135‑1 (MQ=255)
                                           tGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATc                                                                                          <  4:371861/135‑1 (MQ=255)
                                                cAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGttt                                                                                                                                       <  4:235138/86‑1 (MQ=255)
                                                cAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGgtt                                                                                                                                       >  3:235138/1‑86 (MQ=255)
                                                cAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTg                                                                                                                            <  8:189111/97‑1 (MQ=255)
                                                cAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTg                                                                                                                            >  7:189111/1‑97 (MQ=255)
                                                cAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCCCTGGCA‑TCCGGGTTGGTAATgcc                                                                                     >  2:370453/1‑135 (MQ=255)
                                                     cagcaTCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCCCTGGCA‑TCCGTGTTGATCATGCCGCCGa                                                                                >  4:377966/1‑135 (MQ=255)
                                                     cagcaTCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGa                                                                                >  4:216655/1‑135 (MQ=255)
                                                        cttcgtcgATCGGCAGGCTGTGCATGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCGGGCCACTGGAA‑TCCGTGTTGATCATGCCGCCGACCGt                                                                            <  8:323485/134‑1 (MQ=255)
                                                         aTCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGTCA‑TCCGTGTTGATCATGCCGCCGACCGTg                                                                           <  8:297960/136‑1 (MQ=255)
                                                             tcgATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGgcgc                                                                       <  7:406199/136‑1 (MQ=255)
                                                              cgATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGgcgc                                                                       <  4:194587/135‑1 (MQ=255)
                                                              cgATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGgcgc                                                                       <  1:286074/135‑1 (MQ=255)
                                                              cgATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGGCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGGA‑TCCGTGTTGATCATGCCGCCGACCGTGgcgc                                                                       <  1:216338/135‑1 (MQ=255)
                                                                    gCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTg                                                                  <  1:117267/134‑1 (MQ=255)
                                                                       ggCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGt                                                             <  7:161070/136‑1 (MQ=255)
                                                                        gCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGt                                                             <  3:235677/135‑1 (MQ=255)
                                                                        gCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGt                                                             <  3:216655/135‑1 (MQ=255)
                                                                        gCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCGGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGt                                                             <  6:341160/135‑1 (MQ=255)
                                                                        gCTGTGCAGGCGTTCGCCACCGAGCAGCACGCGGTGCAGCTCCAGCACGTGGGCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGt                                                             <  5:68067/135‑1 (MQ=255)
                                                                        gCTGTGCAGGCGGTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGt                                                             <  3:17132/135‑1 (MQ=255)
                                                                             gCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTTCCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCgggtgggggtgggcc                                                        >  1:279278/1‑132 (MQ=255)
                                                                             gCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCC‑TCCGTGGTGATCCTGCCGCCGACCGTGGCGCGGTTGGAGGTGGACa                                                        >  3:373549/1‑135 (MQ=255)
                                                                                ggCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCt                                                     <  6:194494/135‑1 (MQ=255)
                                                                                ggCGGTCGCCACCGAGCAGCACGCTGTGCAGCTCCCGCACGTGGTCGCGGGTTTTACCGTAGGGGCAGCTGCCCTGGCCACTGGCA‑TCCGTGGTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCt                                                     <  2:395989/135‑1 (MQ=255)
                                                                                     tCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAggg                                                <  1:240518/135‑1 (MQ=255)
                                                                                     tCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAggg                                                <  1:343210/135‑1 (MQ=255)
                                                                                         cACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTATGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGGTCATGCCGCCGACCGTGGCGCGGGTGGCGGGGGCCAGCTGAggggg                                              >  3:273394/1‑132 (MQ=255)
                                                                                         cACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGCGGTGGCCAGCTCAGGGGc                                              >  1:122248/1‑133 (MQ=255)
                                                                                         cACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCg                                             >  7:283692/1‑134 (MQ=255)
                                                                                         cACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGGTTTTCCGTTGGTGCAGCTGCCCTGGCCACAGGCC‑TCCCTGGTGGTCATTCCGCCGCCCGGGGCGCGGGTGGGGGTGGGCCGCGCCggggg                                              >  5:431444/1‑132 (MQ=255)
                                                                                                  gcaCGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGcc                                   <  1:111754/135‑1 (MQ=255)
                                                                                                    aCGCTGTGCAGCTCCAGCACGGGGTCGCGGGGTTTACCGGAGGGGCAGCGGCCCTGGCCCCTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGGTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCg                                 <  6:263220/135‑1 (MQ=255)
                                                                                                         gtgCAGCTCCAGCACGGGGTCGCGGGGTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGGTGATCCTGCCGCCGACGGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCgg                            <  6:81852/135‑1 (MQ=255)
                                                                                                          tgCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCgg                            <  7:60712/134‑1 (MQ=255)
                                                                                                           gCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGCCCGTGGCGCGGGTGGAGGTGGACAGCTCAGGGGCGAAGCACCGCCCGTGCGGCt                          >  6:436573/1‑135 (MQ=255)
                                                                                                           gCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCt                          >  4:162193/1‑135 (MQ=255)
                                                                                                             aGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCCCTGGCA‑TCCGGGTTGATCATGCCGCCGCCCGGGGCGCGGTTGGAGGTGGCCCGCGCCGGGGCGCAGAACAGCCCGTGCGGCtt                         >  7:12855/1‑134 (MQ=255)
                                                                                                             aGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTTATAATGCCGCCGACCGTGGGGGGGGTGGCGGGTGTCAGCGCACGGGCGCAGAACAGCCCGTGCGGCGTg                        >  2:111754/1‑135 (MQ=255)
                                                                                                             aGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TACGTGTTGATCATGCCGCCGACCCTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGGAGAACCGCCCGTGCGGCtt                         >  7:84833/1‑134 (MQ=255)
                                                                                                             aGCTCCAGCACGTGGTCGCGGGTTTGAAAGTAGGGGGAGGTGCCCTGTCCACTTGCAGACGGTGTTG‑TCTTTCCGCCGACCGGGGCGGGGGTTGTGGTTGGCATATCATGGTCGGATAAAACCCCTTGCGGCtgt                        >  6:23170/1‑133 (MQ=255)
                                                                                                             aGCTCCAGCACGTGGTCGCGGGGTTTTCCGTAGGTGGAGCTGCCCTGGCCCCGGGCC‑TCCGTGTTGATGATGCCGCCGCCCGGGGCGCGGGTGGGGGTGGACAGCTCCGGGGGGCAGAACCGCCCGTGCGGCttt                        >  4:277032/1‑134 (MQ=255)
                                                                                                             aGCTCCAGCACGTGGTCGCGGGGTTTTCCGTAGGTGGAGCTGCCCTGGCCACTGGCC‑TCCGGGTTGCTCATGCCGCCGCCCCGGGCGCGGGTGGGGGTGGTCAGCTCCGGGGGGGAGACCAGCCCGTGCGGCGTg                        >  5:261304/1‑135 (MQ=255)
                                                                                                              gCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGa                       <  2:349596/135‑1 (MQ=255)
                                                                                                              gCTCCAGCACGTGGTCGCGGGTTTTACCGGATGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTTGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGa                       <  6:422330/135‑1 (MQ=255)
                                                                                                              gCTACAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGa                       <  3:5525/135‑1 (MQ=255)
                                                                                                               cTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGTCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCCGGTGGAGGTGGACAGGTCAGCGGGGCAGAACAGCCCGTGCGGCTTGAgc                     >  7:213404/1‑136 (MQ=255)
                                                                                                                       cGTGGTCGCGGGTTTTTACGTAGGTGGCGCTGCCCTTGCCCCTGGCA‑TCCGTGTTGATCATGCCGCCCACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCCTGCGGCTTGCGCgcagcaa              >  3:161236/1‑134 (MQ=255)
                                                                                                                       cGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTg                                                                  >  4:337163/1‑83 (MQ=255)
                                                                                                                       cGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTg                                                                  <  3:337163/83‑1 (MQ=255)
                                                                                                                       cGTGGTCGCGGGTTTTACCGTAGGGGCAGCTGCCCTGGCCACGGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGGTGGAGGTGGCCAGCGCAGGGGCGTAGAACAGCCCGTGCGGCTTGCGCGCCGCAt              >  7:220772/1‑135 (MQ=255)
                                                                                                                       cGTGGTCGCGGGGTTTTCCGTAGGTGCAGCTGCCCTGGCCCCTGGGA‑TACGTGTTGATCATGCCGCCGCCCGTGGCGCGGGTGGAGGGGGGCAGCTCAGGGGCGCAGAACACCCCGGGCGGCTTGAGCgccgcaa              >  6:38370/1‑134 (MQ=255)
                                                                                                                        gTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGAGCGCAGCATTg            <  8:283692/136‑1 (MQ=255)
                                                                                                                         tGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGGCCGTGCGGCTTGAGCGCAGCATTg            <  6:124443/135‑1 (MQ=255)
                                                                                                                         tGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGAGCGCAGCATTg            <  4:100511/135‑1 (MQ=255)
                                                                                                                         tGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGAGCGCAGCATTg            <  3:377966/135‑1 (MQ=255)
                                                                                                                         tGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGGTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGAGCGCAGCATTg            <  2:396786/135‑1 (MQ=255)
                                                                                                                         tGGTCGCGGGTGTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGAGCGCAGCATTg            <  5:252943/135‑1 (MQ=255)
                                                                                                                            tCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATTCCGCCGACCCTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAAAAGCCCGTGCGGCTTGAGCGCAGCATTGAGCt        >  8:219979/1‑136 (MQ=255)
                                                                                                                            tCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGCCCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGAGCGCAGCATTGAGCt        >  8:33137/1‑136 (MQ=255)
                                                                                                                               cgGGGTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGGGGCGCGGTTGGAGGGGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGAGCGCAGCATTGAGCTgg      <  5:257234/135‑1 (MQ=255)
                                                                                                                                   ttttACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGAGCGCAGCATTGAGCTGGTCCt  <  2:100312/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
CATGCGGTACACCTCGCCGACCCGGCCCGGCGCGGCGCAGGCCTGTTCCAGCGCAGCATCGTCGATCGGCAGGCTGTGCAGGCGTTCGCCACCGAGCAGCACGCTGTGCAGCTCCAGCACGTGGTCGCGGGTTTTACCGTAGGTGCAGCTGCCCTGGCCACTGGCA‑TCCGTGTTGATCATGCCGCCGACCGTGGCGCGGTTGGAGGTGGACAGCTCAGGGGCGAAGAACAGCCCGTGCGGCTTGAGCGCAGCATTGAGCTGGTCCT  >  KT2440/5101293‑5101558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑