| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,298,739 | T→G | 17.8% | D780A (GAC→GCC) | recC ← | RecBCD enzyme subunit RecC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,298,739 | 0 | T | G | 17.8% | 90.1 / 2.1 | 52 | D780A (GAC→GCC) | recC | RecBCD enzyme subunit RecC |
| Reads supporting (aligned to +/- strand): ref base T (12/29); new base G (9/2); total (21/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.85e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCTGACATACAGTTGGTCACGTGCTGACAGTAGCG > KT2440/5298608‑5298871 | aTTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGGCCAGCGGCGCGTC‑gg > 5:86381/1‑135 (MQ=255)aTTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑gg < 5:287372/135‑1 (MQ=255)aTTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑g > 4:16040/1‑134 (MQ=255)aTTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑g < 3:16040/134‑1 (MQ=255)aTTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCCGGCTGGCCCGGCGGCGCGTC‑gg > 6:65124/1‑135 (MQ=255) tGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCaaa > 3:330973/1‑135 (MQ=255) aTAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGGGGGGCCGGCGGGGCGGC‑GGGCCAATggcc > 3:274627/1‑132 (MQ=255) aGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCAGCTGCGCGTC‑GGCCCAAAGCCAGc > 2:346450/1‑135 (MQ=255) aaCAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCCGGCAGGCCCGGCTGCGCGGG‑GGCCCAATgcccgccccccc > 8:296169/1‑132 (MQ=255) aGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCGGGCCCAGCTGCGCGTG‑GGCCAAAAGCCAcccccccccg > 6:247588/1‑129 (MQ=255) gTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGa < 6:425113/135‑1 (MQ=255) gCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGGTGGCCCTGCTGCGCGGC‑GGCCAAAAGCCAGCCCGCCGCGCtgtg > 4:303381/1‑135 (MQ=255) gCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCCGGCGGGGCCGGCGGGGCGGG‑GGCCCAAATCCAGCCCCCCGCGCtgt > 7:7139/1‑134 (MQ=255) ggCCCTTCTGGAAGTACCGCGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGGCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATc < 7:331236/135‑1 (MQ=255) ccTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGGGGGCCCAGCTGCGCGGC‑GGCCCAATGCCAGCCCCCCGCGCTGTGATcgc > 2:329288/1‑135 (MQ=255) tGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCGGGCCCCGCTGCGCGGCGGGCCAAAT‑CCCGCCCCCCGCCCTGTGATCGGGCCACt > 8:301966/1‑136 (MQ=255) gAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCGGGCCCGGCTGCGCGGC‑GGCCAAATGCCCGCCCCCCCCCATGTGATCCCGCCACtc > 6:111142/1‑134 (MQ=255) aaGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAAc < 5:183303/132‑1 (MQ=255) aaGGACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGGCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGGC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAAc < 5:65124/132‑1 (MQ=255) aggaCCGAGGGCTGATCGGCTGCAGCGGGTGCTCCTGGGTCAGGGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGcc < 8:399046/133‑1 (MQ=255) gTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGcc < 3:322658/135‑1 (MQ=255) gTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGcc < 6:151548/135‑1 (MQ=255) gTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCAGCTGCGCGTC‑CGCCCAAATCCACCCCCCCACGCTGTGATAGCGCAACAGGcc > 4:185998/1‑135 (MQ=255) tACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCg < 6:257656/135‑1 (MQ=255) gAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGGC‑GGCCCAATGCCCGCCCGCAGCGATGTGGTGGCGCAACTTGCCgctac > 4:322658/1‑131 (MQ=255) ggCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCAGCGGCGCGTC‑GGCCCAATGCCCGCCCGCCGCGATGTGATCGCGCCACGGGCCGATCAGCACCCCGGcc > 2:308279/1‑135 (MQ=255) cGGGTGCTCCTGGGGCAGTGCGTGCAACAGTTGCTCGCCCTGTCTCGCGGGCTGGCCCTGCTGCGCTTC‑GGCCAAATGCCAGCCCGCAGCGAGGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCg < 8:192137/136‑1 (MQ=255) tCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGGGCGGC‑GGCCCCATGGCCGCCCGCCGCGATGGGCTAGCGCAACAGGGCGATCAGCCCCGCGGCCGGGGGGTCGcgtttg > 4:99327/1‑130 (MQ=255) tGGGGCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTc < 6:415377/134‑1 (MQ=255) cAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGAt < 5:22149/135‑1 (MQ=255) gtgcgtgcAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACt < 8:7139/136‑1 (MQ=255) gtgcgtgcAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCC‑CCGCGCTGTGATCGCGCAACTGGCCGCTCAGCCCCGCGGCCGGGCGGGCGCTATTTTCGCGGGTACt > 2:172649/1‑135 (MQ=255) cgtgcAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCAGCGCGTC‑GGCCCAATGCCCGCCCGCCGCGCTGTGATAGCGCAACTGGCCGATCAGCCCCGCGGCCGGGCGGTCGCTATTGTCGCGGATACTgcg > 8:311682/1‑136 (MQ=255) aaCAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGa < 7:432982/136‑1 (MQ=255) aGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGAcc < 5:400829/135‑1 (MQ=255) aGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGAcc < 4:418092/135‑1 (MQ=255) aGTTGCGCGGCCGGTCTCGCGGGCTGGCCCTGCTGCGCGGC‑GGCGAAATGCCAGCCGGCAGCGATGTGTTCGCGCTACTGGCCGATCAGCACCGAGGCCGGGCGGTCGCTATTGTCGGGGATACTGCGCCCGTccc < 8:196086/136‑1 (MQ=255) tGCTCGCCCGGTCTCGCGGGCGGGGCCTGCGGCGCGTC‑GGGCCAATGCCAGCCCGCCGCGCTGTGGTAGCGCGACGGGCCGCTGCGCACCCCGGCCGGGGGGTGGCGATTGTGGCGGGTGCTGGGCCCGCCCCAg > 2:329733/1‑135 (MQ=255) tCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCt < 6:293001/134‑1 (MQ=255) ggTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCGGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCTGACATAc < 6:86381/135‑1 (MQ=255) tcGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCTGACATACAGtt < 1:161968/135‑1 (MQ=255) cgGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCTGACATACAGTTGGt < 4:185172/135‑1 (MQ=255) cTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCTGACATACAGTTGGTCACGt < 7:296169/136‑1 (MQ=255) tGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCTGACATACAGTTGGTCACGt < 3:185998/135‑1 (MQ=255) tGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCTGACATACAGTTGGTCACGt < 5:247588/135‑1 (MQ=255) cccTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCCACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGCCCCAGCTGACATACAGTTGGTCACGTGca > 4:220231/1‑134 (MQ=255) ctgctgCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCTGACATACAGTTGGTCACGTGCTGAc < 7:178714/136‑1 (MQ=255) tgctgcGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCTGACATACAGTTGGTCACGTGCTGAc < 4:81388/135‑1 (MQ=255) gcgTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCTGACATACAGTTGGTCACGTGCTGACAGTAgc < 1:346450/135‑1 (MQ=255) gcgTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTAATGGCGCGGATACTGCGCCCGACCCAGCTGACATACAGTTGGTCACGTGCTGACAGTa < 4:3045/133‑1 (MQ=255) gcgTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTA‑TGTCGCGGATACTGCGCCCGACCCAGCTGTCATACAGTTGGTCACGTGCTGACAGTAgcg < 4:243971/135‑1 (MQ=255) cgTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCTGACATACAGTTGGTCACGTGCTGACAGTAgcg < 5:99548/135‑1 (MQ=255) | ATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTC‑GGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCAGCACCGAGGCCGGGCGTTCGCTATTGTCGCGGATACTGCGCCCGACCCAGCTGACATACAGTTGGTCACGTGCTGACAGTAGCG > KT2440/5298608‑5298871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |