Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	5,499,534	T→G	29.8%	S179A (TCG→GCG)	CJ019_05176 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,499,534	0	T	G	29.8%	62.3 / 4.6	63	S179A (TCG→GCG)	CJ019_05176	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (22/20);  new base G (2/19);  total (24/39)
Fisher's exact test for biased strand distribution p-value = 9.22e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGATGGTCGGCACGGTCGGTGACCGGCTGGTGGAACTGGCTGCGGGCTGGGGCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCGGCATGACCTGGACCGGCTTGTGGGGCAAGCAGTACGCTGATTTGT  >  KT2440/5499401‑5499666                                                                                                                                      |                                                                                                                                     gCTGATGGTCGGCACGGTCGGTGACCGGCTGGTGGAACTGGCTGCGGGCTGGGGCATCGTGCGGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGGGGCGGTc                                                                                                                                     >  5:59986/1‑135 (MQ=255)    gATGGTCGGCACGGTCGGTGACCGGCTGGTGGAACTGGCTGCGGGCTGGGGCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGGGGTCGgc                                                                                                                                  >  3:357683/1‑135 (MQ=255)    gATGGTCGGCACGGTCGGTGACCGGCTGGTGGAACTGGCTGCGGGCTGGGGCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCGGTCGgc                                                                                                                                  >  1:83418/1‑135 (MQ=255)      tGGTCGGCACGGTCGGTGACCGGCTGGTGGAACTGGCTGCGGGCTGGGGCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGgcgc                                                                                                                                >  6:175200/1‑135 (MQ=255)        ggcGGCACGGGCGGTGCCCGGCTGGGGGAACTGGCTGCGGGCGGGGGCATCGTGCGGGTGGTAGCGGGCCGGTACCTGTGGGGGCCGCGCGGGCGCAGGGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGcgcgcg                                                                                                                             <  8:191279/134‑1 (MQ=255)           ggCACGGTCGGTGACGGGCTGGTGGAACTGGCTGCGGGCGGGGGCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGcggc                                                                                                                           <  1:375415/135‑1 (MQ=255)              aCGGTCGGTGACCGGCTGGTGGAACTGGCTGCGGGCTGGGGCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCAGTAGGCGCGcggccgg                                                                                                                        >  2:96585/1‑135 (MQ=255)               cggtcggtGACCGGCTGGTGGAACTGGCGGCGGGCTGGGGCAGCGGGCTGGTGGTAGCGGGCCGGTACCTGGGGGGGCCGCGCGGGCGCAGGGGCGCGGGCGTGCTTTGGCCGCGGCTGGCGGCGCGCGGCCGGCt                                                                                                                      <  7:49686/136‑1 (MQ=255)               cgggcgggGACCGGCTGGTGGACCTGGCTGCGGGCGGGGGCAGCGTGCTGGGGGTAGCGGGCCTGTACCTGTGGGGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCt                                                                                                                      <  7:22015/136‑1 (MQ=255)                ggtgggtgACGGGCTGGGGTACCTGGCTGGGGGCGGGGGCATCGTGCGGGGGGTATCGGGCCTGTACCTGGGGGGGCCGCGCGGGCGCAGGGGGGCTGGCGTGCTTTGGCGGCGGCTGGCGGCGCGCGGCCGGCt                                                                                                                      <  6:291382/135‑1 (MQ=255)                ggtcggtGACCGGCTGGTGGAACTGGCTGCGGGCTGGGGCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCTGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCt                                                                                                                      <  1:290837/135‑1 (MQ=255)                ggtcggtGACCGGCTGGTGGAACTGGCTGCGGGCTGGGGCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTg                                                                                                                     >  8:245487/1‑136 (MQ=255)                     gtGACCGGCTGGTGGAACTGGCTGCGGGCTGGGGCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGGTTTGGGCGCGGGTGTCGGCGCGCGGCCGGCGGtgag                                                                                                                 >  2:404332/1‑132 (MQ=255)                               ggtggAACTGGCTGCGGGCTGGGGCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGCCCt                                                                                                       >  4:179226/1‑135 (MQ=255)                               ggtggAACGGGCGGCGGGCTGGGGAAGCGTGCGGGTGGTAGCGGGCCTGTACCTGGGGTGGCCGCGCGGGCGCAGGGGCGCGGGCGTGCTTTGGCCGCGGCTGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCt                                                                                                       <  5:451182/135‑1 (MQ=255)                               ggtgcAACGGGCTGCGGGCGGGGGCATCGGGCTGGGGGTTGCGGGCCTGTACCTGTGGTGGCGGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCt                                                                                                       <  1:236553/135‑1 (MQ=255)                                   gAACTGGCTGCGGGCTGGGACATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCAc                                                                                                   >  4:228715/1‑135 (MQ=255)                                     acgggCTGCGGGCTGGGGCAGCGGGCTGGTGGTATCGGGCCTGTACCTGGGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCAcgcg                                                                                                <  7:212052/133‑1 (MQ=255)                                       tGGCTGCGGGCTGGGGCAGCTGGCGGGGGGTATCGGGCCGGTACCTGTGGTGGCCGCGCGGGCGCAGGGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCgg                                                                                               <  3:335963/135‑1 (MQ=255)                                         gcggcgggcggGGGCAGCGTGCTGGTGGTAGCGGGCCTGTACCTGTGGGGGCCGCGCGGGCGCAGTGGCGCGGGCGGGCTTTGGCGGCGGCGGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTa                                                                                             <  3:152625/132‑1 (MQ=255)                                         gCTGCGGGCTGGGGCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCCGCCGGCTGTTCTGGCGATACCTGCAAGCGGTa                                                                                             >  4:165767/1‑135 (MQ=255)                                               ggCGGGGGCATCGTGGGGGTGGTATCGGGCGTGTCCCTGTGGTGGCCGCGCGGGCGCAGGGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGt                                                                                       <  1:49235/135‑1 (MQ=255)                                                      gCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGGGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTggg                                                                                <  4:130519/135‑1 (MQ=255)                                                      gCATCGTGCTGGTGGTAGCGGGCCTGTACCTGTGGGGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTggg                                                                                <  6:99480/135‑1 (MQ=255)                                                             gCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTTGCCGCGGCTGTAGGCCCGCCGCCCGCGGTTATGGGGAGCACGGCCAGCGGGAACCCGGTTGAGGGGGTCggg                                                                         >  3:242530/1‑134 (MQ=255)                                                              cTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTGGGCCGCGGCTGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACACGGTAACCGGTTTCTGGGGTTCGGcc                                                                        <  4:419317/135‑1 (MQ=255)                                                                ggtggtCGCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGGGCTTTGGCCGCGGCTGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCtt                                                                      <  6:196459/135‑1 (MQ=255)                                                                ggtggtATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCtt                                                                      <  4:59188/135‑1 (MQ=255)                                                                        cGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCtgttg                                                               <  2:83418/134‑1 (MQ=255)                                                                          ggCCTGTACCTGTGGGGGCCGCGCGGGCGCAGTGGGGCGGGCGGGCTTTGGCCGCGGCGGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGttgttg                                                            <  6:59986/135‑1 (MQ=255)                                                                          ggCCGGTCCCTGTGGGGGCCGCGCGGGCGCAGGGGCGCGGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGttgttg                                                            <  3:120279/135‑1 (MQ=255)                                                                                tcccTGGGGTGGCCGCGCGGGGGCTGGGGCGCGGGCGTGCTTTGGCGGGGGGTGGCGGCGCGGGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTgg                                                     <  8:285379/134‑1 (MQ=255)                                                                                 acgtgtgGGGGCCGCGCGGGCGCAGGGGGGCTGGCGTGCTTTGGCCGCGGCTGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTgg                                                     <  1:376625/132‑1 (MQ=255)                                                                                     ggggtggCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGc                                                <  8:95547/134‑1 (MQ=255)                                                                                      gggtggCCGCGGGGGCGCAGTGGCGCGGGGGGGCTTTGGCCGCGGCGGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGc                                                <  1:96585/134‑1 (MQ=255)                                                                                      gggtggCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGGGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGc                                                <  5:229116/134‑1 (MQ=255)                                                                                      ggggggcggcGCGGGCGCTGGGGGGCTGGGGTGCTTTGGCCGGGGCTGTGGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGc                                                <  3:9167/131‑1 (MQ=255)                                                                                      ggggggCCGCGCGGGCGCTGGGGCGCGGGCGTGCTTTGGCCGCGGCGGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCg                                               <  8:247002/132‑1 (MQ=255)                                                                                       gggggCCGGGCGGGCGATGGGGGGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCg                                               <  6:376892/132‑1 (MQ=255)                                                                                       gggggCCGCGCGGGCGCAGTGGAGGTGGCGTGGTTGGGCGGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCg                                               <  4:286367/132‑1 (MQ=255)                                                                                        gtggCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGTCCCGCGGTTCTGGCGGGGCCAGCCCGGGGGAACCGGTTTGTGGGGTTCGGCCGTGCTGTTGTTGGTGGTGGTCAGCg                                               >  1:157890/1‑134 (MQ=255)                                                                                        gtggCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTggg                                                                                >  8:359728/1‑101 (MQ=255)                                                                                        gtggCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTggg                                                                                <  7:359728/101‑1 (MQ=255)                                                                                        gtggCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCAGCCCGCGGTGACCGGGTTCTGGGGTTCGGCCGTGGTGTTGTTGATGCTGGTAAGCGGc                                             >  7:285379/1‑136 (MQ=255)                                                                                        gtggCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCGGTTCTGGCGAGCCCTGCCCGCGGTTACCGGGTTCTGGGGGTGGGCCTTGCGGTTGGTGATGCTGGTCAGCgg                                              >  4:48389/1‑135 (MQ=255)                                                                                           gCCGCGGGGGGGCTGGGGCGGTGGGGTGCTTTGGCGGGGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCGGCAt                                           <  3:208847/135‑1 (MQ=255)                                                                                           gCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCCCGGCTGTCGGCGCGCGGCCGGCGGTTGTGGCGAGACCTGCCCGCGGTAACCCGGTTCTGGGGGTCGGGCTTGGGGTTGGTTATGCTGGTCAGCGGCAt                                           >  8:22999/1‑135 (MQ=255)                                                                                             cgcgcgGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGGTCTGGCGCGACCTGCCCGCGGGGACCGGGTTCTGGGGGTGGGGCGTGGGGGTGTTGGTGGTGGGCCGCGGCATggc                                        >  7:407728/1‑134 (MQ=255)                                                                                              gcgcgGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGGGGTCGGCGCGCGGCCGGCGGTTCTGGCGAGACCTGCCCGCGGTTACCGGTGTCTGGGGTTCGGGCTTTCTGTTTTTGATGGTGGTGAGCGGCATGaac                                       >  7:251804/1‑134 (MQ=255)                                                                                               cgcgGGCGCAGTGGCGCTGGCGTGCTTTTGCCGCGGCAGTGGGCGCCCGGCCCGCGGTTATGGCGAGCCCGGCACGCCGGAACCGGTTTGTGGGGTTGGGCCGTGCTGTTGTTGCTGCGGGGGAGCGGccttgcc                                       >  2:306485/1‑129 (MQ=255)                                                                                                gcgGGCGCAGTGGCGCTGGGGTGCTTTGGCCGCGGCTGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCGGCATGACCt                                      <  6:211225/135‑1 (MQ=255)                                                                                                gcgGGCGCAGTGGCGCGGGCGGGCTGTGGCGGCGGCTGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCGGCATGACCTg                                     <  7:100725/136‑1 (MQ=255)                                                                                                        aggggCGCTGGCGTGCTGTGGCCGGGGCTGGCGGCGCGCGGCCGGCTGTGCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTGCGGCCTTGCTGTTGTGGATGCTGGTCAGCGGCATGACCTGGACCGGCt                             <  7:75168/133‑1 (MQ=255)                                                                                                         ggggCGCTGGCGTGCTTTGGCCGCGGCTGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCGGCATGACCTGGACCGGCt                             <  4:87002/133‑1 (MQ=255)                                                                                                              gcgggCGTGCTTTGGCCGCGGCGGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCGGCATGACCTGGACCGGCTTGTgg                        <  6:336222/132‑1 (MQ=255)                                                                                                              gcgggCGTGCTTGGGCCGGGGCTGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCGGCATGACCTGGACCGGCTTGTgg                        <  3:179226/132‑1 (MQ=255)                                                                                                              gcTGGCGTGCTTTGGCCGCGGCTGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTGCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCGGCATGACCTGGACCGGCTTGTgg                        <  3:133210/135‑1 (MQ=255)                                                                                                                 ggCGTGCTTTGGCCGCGGGGGGCGGCGCGCGGCCGGGTGGTGTGGGGGGCCCTGCACGCGGGTACCGGGTTGTGGGGTTCGGGCGTGGGGGTGTTGATGCTGGTCGGCGGGCTGGCCGGGGCCGGGGTGTgggg                      >  1:7412/1‑134 (MQ=255)                                                                                                                 ggCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGGTGTTCTGGCGAGACCTGCACGCGGGTACCGGGTTGTGGGGGTGGGGCGTGCTGGTGTTGTTGCTGGTCAGCGGCATGACCTGGGCCGGCGTGTgggg                      >  3:99998/1‑134 (MQ=255)                                                                                                                  gggTGCTTTGGCCGCGGCTGGCGGCGCGCGGCCGGCTGTTCTGGCGAGCCCTGCACGCGGGCACCGGGGTCTGGGGGTCGGGCGTGCTGTTGTTGCTGCTGGTCAGCGGCCTGACCGGGTCCGGCTGGTGGGGc                     >  7:355288/3‑134 (MQ=255)                                                                                                                     tGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGGTGTTGTGGTGTGGCCTGCACGCGGTTACCGGTTTGTGGGGGTCGGGCGTGCGGTTGTTGATGCTGGTGAGCGGCATGGCCTGGGCCGGCTTGTgggggcag                  >  8:390784/1‑130 (MQ=255)                                                                                                                        tttGGCCGCGGCGGGCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCGGCATGACCTGGACCGGCTTGTGGGGCAAGCAGt              <  3:408864/135‑1 (MQ=255)                                                                                                                                    tGTCGGCGCGCGGCCGGCTGTTATGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCGGCATGACCTGGACCGGCTTGTGGGGCAAGCAGTACGCTGATTtgt  >  3:69072/1‑135 (MQ=255)                                                                                                                                      |                                                                                                                                     GCTGATGGTCGGCACGGTCGGTGACCGGCTGGTGGAACTGGCTGCGGGCTGGGGCATCGTGCTGGTGGTATCGGGCCTGTACCTGTGGTGGCCGCGCGGGCGCAGTGGCGCTGGCGTGCTTTGGCCGCGGCTGTCGGCGCGCGGCCGGCTGTTCTGGCGAGACCTGCACGCGGTAACCGGTTTCTGGGGTTCGGCCTTGCTGTTGTTGATGCTGGTCAGCGGCATGACCTGGACCGGCTTGTGGGGCAAGCAGTACGCTGATTTGT  >  KT2440/5499401‑5499666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑