Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	5,633,108	T→A	5.4%	L124Q (CTG→CAG)	CJ019_05291 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,633,108	0	T	A	5.4%	243.9 / 3.5	74	L124Q (CTG→CAG)	CJ019_05291	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (41/29);  new base A (2/2);  total (43/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGCCAACAGTTTCAACGTCGACTGG  >  KT2440/5632981‑5633241                                                                                                                                |                                                                                                                                      gTTGCGCTCGAGCAGCTGCGCCAGACCTAGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTg                                                                                                                                <  1:171283/135‑1 (MQ=255)      gcTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGa                                                                                                                           >  1:132027/1‑135 (MQ=255)      gcTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGa                                                                                                                           >  3:317245/1‑135 (MQ=255)             cagcTGCGACAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGAAGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAg                                                                                                                   <  7:190185/136‑1 (MQ=255)              agcTGCGCCAGACCTTGCCGCTGCGGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAg                                                                                                                   <  2:421734/135‑1 (MQ=255)               gcggctccAGACCTTGCCGCTGCTGGCGATCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGc                                                                                                                  <  2:198913/129‑1 (MQ=255)               gcTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGc                                                                                                                  <  5:144716/135‑1 (MQ=255)               gcTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGc                                                                                                                  <  2:132027/135‑1 (MQ=255)               gcTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCGTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGc                                                                                                                  <  4:236396/135‑1 (MQ=255)               gcTGCGCCAGACCTTGCCGCTGCTGGCGCGCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGc                                                                                                                  <  2:321751/135‑1 (MQ=255)                                cGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGCGCGGGAAGCAGGTGacctggacctgg                                                                                                 >  4:239359/1‑135 (MQ=255)                                cGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGacctggacctgg                                                                                                 >  3:359990/1‑135 (MQ=255)                                cGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGacctggacctgg                                                                                                 >  6:135493/1‑135 (MQ=255)                                 gctgctGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCACGCCCGGAACGAACTACTGGCCCTGCTCGAGCGTGAAGCAGGGGACCTGGACCTGGt                                                                                                >  4:179619/1‑135 (MQ=255)                                                 ccTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACgccgcc                                                                                <  1:362458/135‑1 (MQ=255)                                                 ccTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACgccgcc                                                                                <  4:346330/135‑1 (MQ=255)                                                           gcCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTAt                                                                      <  4:443803/135‑1 (MQ=255)                                                           gcCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCACACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTAt                                                                      <  1:34064/135‑1 (MQ=255)                                                             cTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGACCCCCCCCTGCCCGCACTCAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCACGAGACCTCGACCTGGTCCGCATCTACGCCGCCGGCCCGGTATGcc                                                                   >  7:81841/1‑136 (MQ=255)                                                             cTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGc                                                                    >  1:50741/1‑135 (MQ=255)                                                             cTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGc                                                                    >  1:50739/1‑135 (MQ=255)                                                             cTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGc                                                                    >  6:226222/1‑135 (MQ=255)                                                             cTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGc                                                                    >  5:110230/1‑135 (MQ=255)                                                             atGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCACTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGAATGc                                                                    >  4:262403/2‑135 (MQ=255)                                                                gACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCcgc                                                                 <  7:13492/135‑1 (MQ=255)                                                                       aCGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCg                                                          >  4:304362/1‑135 (MQ=255)                                                                                      aTAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGgcg                                           <  6:182458/135‑1 (MQ=255)                                                                                      aTAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGgcg                                           <  5:399510/135‑1 (MQ=255)                                                                                      aTAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCGTCGCCAGCTCGTTCGgcgc                                          >  7:105942/1‑136 (MQ=255)                                                                                       tAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTAt                                                                      <  1:288744/107‑1 (MQ=255)                                                                                       tAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTAt                                                                      >  2:288744/1‑107 (MQ=255)                                                                                               aGGACCCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCCGCTCGTTCGGCGCCGTCGGcg                                  >  1:95785/1‑134 (MQ=255)                                                                                               aGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGccgccg                                                                               <  2:107194/90‑1 (MQ=255)                                                                                               aGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGccgccg                                                                               >  1:107194/1‑90 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAGGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTgg                                <  3:183554/135‑1 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTgacctggacctg                                                                                                  >  8:69014/1‑69 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTgacctggacctg                                                                                                  <  4:312911/69‑1 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTgacctggacctg                                                                                                  <  7:69014/69‑1 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTgacctggacctg                                                                                                  >  3:312911/1‑69 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGCCCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGc                               >  7:125112/1‑136 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGGGTCGCCCGCTCCTTCGGCGCCGGCGGCgggg                               >  8:387409/1‑135 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCGTACGGCTgg                                >  7:21223/1‑135 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTgg                                >  1:65078/1‑135 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGc                               >  7:331633/1‑136 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCCCCGTCCGCTgg                                >  4:4149/1‑135 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGGTCGGCGCCGTCGGCTgg                                >  2:172417/1‑135 (MQ=255)                                                                                                 gAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCCTCCCCAGCTCGGTCGGCGCCTTCGGCAgg                                >  5:450065/1‑135 (MQ=255)                                                                                                      cccccTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGTACCTGGTCGGCATCTACGCAGCCGGCCCGGTATGCCGCGTCTTCGCACGCTCGTGCGGAGCCTGAGGCTGGCACCa                           >  6:366242/1‑135 (MQ=255)                                                                                                       ccccTGCCCGCACTGAACGAAGTACAGGCCCTGCTCGAGCGTGAAGCAGGTg                                                                                                             <  1:265564/52‑1 (MQ=255)                                                                                                       ccccTGCCCGCACTGAACGAAGTACAGGCCCTGCTCGAGCGTGAAGCAGGTg                                                                                                             <  1:258370/52‑1 (MQ=255)                                                                                                       ccccTGCCCGCACTGAACGAAGTACAGGCCCTGCTCGAGCGTGAAGCAGGTg                                                                                                             >  2:265564/1‑52 (MQ=255)                                                                                                       ccccTGCCCGCACTGAACGAAGTACAGGCCCTGCTCGAGCGTGAAGCAGGTg                                                                                                             >  2:258370/1‑52 (MQ=255)                                                                                                        cccTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAgg                         <  1:306510/135‑1 (MQ=255)                                                                                                         ccTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGc                        <  3:60773/135‑1 (MQ=255)                                                                                                            gCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTAGCCACCTCGTTAGGCGCCTGCGGCGGGCCCCAGGCCa                      >  3:124352/1‑134 (MQ=255)                                                                                                            gCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGGATGCCGCGGCGTCGCCAGCGCGGTCGGCGCCGGCGGCGGGCCCCCGGccca                     >  5:330869/1‑133 (MQ=255)                                                                                                              ccGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGCCAACAg                  >  7:216981/1‑136 (MQ=255)                                                                                                               cGCACTGAAGGAAGTACTGGCCCTGGTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGCCAACAg                  <  1:61487/135‑1 (MQ=255)                                                                                                                    tGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCCGCTCGTTCGGCGCCTTCGGCTGGCACCCGGCCAACAGTTTcc             >  2:351702/1‑134 (MQ=255)                                                                                                                    tGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTAGCCAGCTCGTTCGGCGCCTTCGGCTGGCCCCAGGCCACCAGtttaa             >  6:6940/1‑133 (MQ=255)                                                                                                                       aCGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGCCAACAGTTTCaa            <  6:110230/133‑1 (MQ=255)                                                                                                                       aCGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGCCAACAGTTTCaa            <  4:317245/133‑1 (MQ=255)                                                                                                                       aCGAAGTACTGGCCCTGCGCGAGCGTGAAGCAGGTGACCTGGACCTGGGCGGCAGCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGCCAACAGTTTCaa            <  1:340134/133‑1 (MQ=255)                                                                                                                         gAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCCGGCCCACAGTTTCAACGTc        >  3:303677/1‑135 (MQ=255)                                                                                                                         gAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGCCAACAGTTTCAACGTCg       <  7:153292/136‑1 (MQ=255)                                                                                                                         gAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGCCAACAGTTTCAACGTCg       >  8:49032/1‑136 (MQ=255)                                                                                                                         gAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCGTCGGCTGGCACCAGGCCAACAGTTTCAACGt         >  6:438782/1‑134 (MQ=255)                                                                                                                         gAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCACGTTCGGCGACGTAGGCTGGCACCACGCCAACAGTTTCAAAGt         >  4:294442/1‑134 (MQ=255)                                                                                                                          aaGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGCCAACAGTTTCAACGTCg       <  4:14866/135‑1 (MQ=255)                                                                                                                          aaGTACTGGCCCTGCTCGAGCGGGAAGCAGGTGACCTGGAGCTTGTCGGCATCTACGGCGCCGTCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGCCAACAGTTTCAACGTCg       <  3:51259/135‑1 (MQ=255)                                                                                                                              aCTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGCCAACAGTTTCAACGTCGACTgg  >  7:47706/1‑136 (MQ=255)                                                                                                                              aCTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCCGCATCTACGCCGCCGGCCCGGTATGCCGCGCCGTCGGCACCTCGTTCGGCGCCGTCGGCTGGCCCCAGCCCAACAGCTTTCACGtggccga   >  5:115528/1‑129 (MQ=255)                                                                                                                               cTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCCCCAGGCCAACAGTTTCAACGTCGACt    >  4:89699/1‑133 (MQ=255)                                                                                                                               cTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCGTCGCCCGCTCGTTAGGCGCCATCGGCTGGCCCCAGGCCCACACTTTCAACGGCgccagg  >  1:223065/1‑130 (MQ=255)                                                                                                                                |                                                                                                                                      GTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGAACCCCCCCTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGGCACCAGGCCAACAGTTTCAACGTCGACTGG  >  KT2440/5632981‑5633241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑